The DOI matches its Crossref record; 84 of the 86 references it cites were found in a registry or an index.
Deoxyribonucleic Acid Profiling Analysis of 40 Human Thyroid Cancer Cell Lines Reveals Cross-Contamination Resulting in Cell Line Redundancy and Misidentification
Open accessAbstract
CONTEXT: Cell lines derived from human cancers provide critical tools to study disease mechanisms and develop novel therapies. Recent reports indicate that up to 36% of cell lines are cross- contaminated. OBJECTIVE: We evaluated 40 reported thyroid cancer-derived cell lines using short tandem repeat and single nucleotide polymorphism array analysis. RESULTS: Only 23 of 40 cell lines tested have unique genetic profiles. The following groups of cell lines are likely derivatives of the same cell line: BHP5-16, BHP17-10, BHP14-9, and NPA87; BHP2-7, BHP10-3, BHP7-13, and TPC1; KAT5, KAT10, KAT4, KAT7, KAT50, KAK1, ARO81-1, and MRO87-1; and K1 and K2. The unique cell lines include BCPAP, KTC1, TT2609-C02, FTC133, ML1, WRO82-1, 8505C, SW1736, Cal-62, T235, T238, Uhth-104, ACT-1, HTh74, KAT18, TTA1, FRO81-2, HTh7, C643, BHT101, and KTC-2. The misidentified cell lines included the DRO90-1, which matched the melanoma-derived cell line, A-375. The ARO81-1 and its derivatives matched the HT-29 colon cancer cell line, and the NPA87 and its derivatives matched the M14/MDA-MB-435S melanoma cell line. TTF-1 and Pax-8 mRNA levels were determined in the unique cell lines. CONCLUSIONS: Many of these human cell lines have been widely used in the thyroid cancer field for the past 20 yr and are not only redundant, but not of thyroid origin. These results emphasize the importance of cell line integrity, and provide the short tandem repeat profiles for a panel of thyroid cancer cell lines that can be used as a reference for comparison of cell lines from other laboratories.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“Similarly, Schweppe and colleagues showed that nearly 50% of thyroid cell lines were misidentified [13].”
From Genetic Insights into the Economic Toll of Cell Line Misidentification: A Comprehensive Review (2026) · cites this paper as [13]
Experimental — accuracy not yet measuredIn the citing paper
“Cell lines were authenticated by short tandem repeat (STR) DNA profiling, and matched with their respective STR profiles as shown in previously published studies, and/or in the European Collection for Cell Cultures (ECACC) and the German Collection of Microorganisms and Cell Cultures (DSMZ) databases at all loci tested in common [48].”
From Restoration of p53 using the novel MDM2-p53 antagonist APG115 suppresses dedifferentiated papillary thyroid cancer cells (2017) · cites this paper as [48]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
2 retracted
Melillo, R. M., Castellone, M. D., Guarino, V., Falco, V. D., Cirafici, A. M., Salvatore, G., Caiazzo, F., Basolo, F., Giannini, R., Kruhøffer, M., et al. (2016) · Journal of Clinical Investigation · doi:10.1172/jci87345 · Cited by 305
RetractedMelillo, R. M., Castellone, M. D., Guarino, V., Falco, V. D., Cirafici, A. M., Salvatore, G., Caiazzo, F., Basolo, F., Giannini, R., Kruhøffer, M., et al. (2005) · Journal of Clinical Investigation · doi:10.1172/jci22758 · Cited by 298
RetractedMutations of the BRAF gene in human cancer
Davies, H., Bignell, G. R., Cox, C., Stephens, P., Edkins, S., Clegg, S., Teague, J., Woffendin, H., Garnett, M. J., Bottomley, W., et al. (2002) · Nature · doi:10.1038/nature00766 · Cited by 10,674
Giard, D. J., Aaronson, S. A., Todaro, G. J., Arnstein, P., Kersey, J. H., Dosik, H., & Parks, W. P. (1973) · JNCI Journal of the National Cancer Institute · doi:10.1093/jnci/51.5.1417 · Cited by 2,336
Kimura, E. T., Nikiforova, M. N., Zhu, Z., Knauf, J. A., Nikiforov, Y. E., & Fagin, J. A. (2003) · PubMed · Cited by 1,510
Self-citationNikiforova, M. N., Kimura, E. T., Gandhi, M., Biddinger, P. W., Knauf, J. A., Basolo, F., Zhu, Z., Giannini, R., Salvatore, G., Fusco, A., et al. (2003) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2003-030838 · Cited by 1,036
Self-citationFogh, J., & Trempe, G. (1975) · Springer eBooks · doi:10.1007/978-1-4757-1647-4_5 · Cited by 1,034
Molecular characterization of human prostate carcinoma cell lines
van Bokhoven, A., Varella‐Garcia, M., Korch, C. T., Johannes, W. U., Smith, E. E., Miller, H. L., Nordeen, S. K., Miller, G. J., & Lucia, M. S. (2003) · The Prostate · doi:10.1002/pros.10290 · Cited by 635
Self-citationClinical Implication of Hot SpotBRAFMutation, V599E, in Papillary Thyroid Cancers
Namba, H., Nakashima, M., Hayashi, T., Hayashida, N., Maeda, S., Rogounovitch, T., Ohtsuru, A., Saenko, V., Kanematsu, T., & Yamashita, S. (2003) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2003-030305 · Cited by 532
Short tandem repeat profiling provides an international reference standard for human cell lines
Masters, J. R., Thomson, J. A., Daly-Burns, B., Reid, Y. A., Dirks, W. G., Packer, P., Toji, L. H., Ohno, T., Tanabe, H., Arlett, C. F., et al. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.121616198 · Cited by 478
Flagged first, then by citations
2 of the 86 references couldn’t be matched to a known paper.
Research works printed but not linked: 2
- Hay, 1984, Banking and strain data of cell cultures., In vitro TCA Monograph, 5, 215
- Ain, 1992, Characterization of an immortalized cell-line (KAK-1) from a benign human thyroid follicular adenoma, Thyroid, 2, S49
Cited by
Sorted by citations
Items 501–565 of 565
Passaro, C., Somma, S. D., Malfitano, A. M., & Portella, G. (2018) · International Journal of Endocrine Oncology · doi:10.2217/ije-2017-0028 · Cited by 2
Toraih, E. A., Hussein, M. H., Fawzy, M. S., & Kandil, E. H. (2022) · Cancers · doi:10.3390/cancers14194728 · Cited by 2
Application of RapidHIT™ ID for cell authentication by fast and convenient STR profiling
Koh, U. N., Lee, J. H., Kang, H. J., Joo, K. M., Lee, J. C., & Lim, S. (2023) · Genes & Genomics · doi:10.1007/s13258-023-01388-4 · Cited by 2
p90RSK Regulates p53 Pathway by MDM2 Phosphorylation in Thyroid Tumors
Maietta, I., Peschio, F. D., Buonocore, P., Viscusi, E., Laudati, S., Iannaci, G., Minopoli, M., Motti, M. L., & Falco, V. D. (2023) · Vide Leaf, Hyderabad eBooks · doi:10.37247/pacr3ed.3.23.3 · Cited by 2
Rose, M. M., Nassar, K. W., Sharma, V., & Schweppe, R. E. (2023) · Medical Oncology · doi:10.1007/s12032-023-02118-2 · Cited by 2
Self-citationKalampounias, G., Varemmenou, A., Aronis, C., Mamali, I., Shaukat, A., Chartoumpekis, D. V., Katsoris, P., & Michalaki, M. A. (2024) · Cancers · doi:10.3390/cancers16142604 · Cited by 2
Kang, H. J., Youn, Y., Hong, M.-K., & Kim, L. S. (2011) · Journal of Korean Medical Science · doi:10.3346/jkms.2011.26.10.1398 · Cited by 2
Gérard, A.-C., Humblet, K., Wilvers, C., Poncin, S., Derradji, H., de Goyet, C. D. V., Abou‐El‐Ardat, K., Baatout, S., Sonveaux, P., Denef, J., et al. (2012) · Thyroid · doi:10.1089/thy.2011-0387 · Cited by 2
Branigan, G. P., Casado‐Medrano, V., O’Neill, A., Ricarte‐Filho, J. C., Massoll, N. A., Salwen, M., Spangler, Z., Scheerer, M., Williamson, E. K., Bauer, A. J., et al. (2023) · Cancers · doi:10.3390/cancers15030879 · Cited by 2
Grassi, E., Dicitore, A., Negri, I., Borghi, M. O., Vitale, G., & Persani, L. (2016) · Endocrine · doi:10.1007/s12020-016-1057-8 · Cited by 2
Bohm, M. S., Dame, M. K., Boyd, J. R., Su, K., Wu, A., Attili, D., Chu, V., Colacino, J. A., & Spence, J. R. (2020) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2020.587107 · Cited by 2
Balas, E. A. (2018) · Wiley eBooks · doi:10.1002/9781119225898.ch6 · Cited by 2
Iosco, C. (2012) · AMS Dottorato Institutional Doctoral Theses Repository (University of Bologna) · doi:10.6092/unibo/amsdottorato/4797 · Cited by 1
Importance of Epigenetic Changes in the Thyroid Cancer Incidence and Their Therapeutic Applications
Zarkesh, M., Taghadosi, M., Azizi, F., Zadeh–Vakili, A., & Hedayati, M. (2014) · Cited by 1
Using CRISPR/Cas9 to Edit a Thyroid Cancer Cell Line
Fuziwara, C. S., & Kimura, E. T. (2023) · Advances in experimental medicine and biology · doi:10.1007/978-3-031-33325-5_5 · Cited by 1
Schweppe, R. E., & Haugen, B. R. (2011) · Thyroid · doi:10.1089/thy.2011.2109.ed · Cited by 1
Self-citationDeen, S., Griffith, O. L., Masoudi, H., Gown, A. M., Jones, S. J. M., & Wiseman, S. M. (2009) · PubMed · Cited by 1
Study of 6-propyl-2-thiouracil as a radioprotector for the thyroid gland
Perona, M., Dagrosa, A., Pagotto, R., Casal, M., Pignataro, O. P., Pisarev, M. A., & Juvenal, G. J. (2010) · International Journal of Low Radiation · doi:10.1504/ijlr.2010.036962 · Cited by 1
Landa, I., Boullosa, C., Inglada-Pérez, L., Sastre-Perona, A., Pastor, S., Velázquez, A., Mančíková, V., Ruiz‐Llorente, S., Schiavi, F., Marcos, R., et al. (2013) · PLoS ONE · doi:10.1371/annotation/cd94c3eb-70f2-4dfa-85be-b3fc41e495c3 · Cited by 1
(2009) · Current Opinion in Oncology · doi:10.1097/cco.0b013e328334b4d9 · Cited by 1
Gordon, H. V. (2012) · Scholarship @ Claremont (The Claremont Colleges) · Cited by 1
Diagnose und Beseitigung von Kontaminationen
Schmitz, S. (2011) · Spektrum Akademischer Verlag eBooks · doi:10.1007/978-3-8274-2573-7_11
Mouse Models of Thyroid Neoplasia: Insights into Thyroid Pathophysiology
Antonello, Z. A., & Nucera, C. (2014) · Elsevier eBooks · doi:10.1016/b978-0-12-386456-7.03704-7
Influência do MicroRNA let-7 e miR-17-92 como oncomiRs no câncer.
Fuziwara, C. S. (2010) · Digital Library of Theses and Dissertations (Universidade de São Paulo) · doi:10.11606/d.42.2010.tde-27092010-152352
Thyroid Cellular Models: A Warning Concerning Their Application
Vitale, M., & Bifulco, M. (2009) · Thyroid · doi:10.1089/thy.2009.0129
Johnson, M. (2012) · Materials and Methods · doi:10.13070/mm.en.2.145
Hormonal regulation of immune modulators in human breast tissue
Morad, V. (2015) · Linköping University medical dissertations · doi:10.3384/diss.diva-117983
Effects of α-lipoic Acid on Differentiation of Thyroid Cancer Cells
Kim, W. G., Han, D. H., Choi, H.-J., Kim, E. Y., Kim, T. Y., Shong, Y. K., & Kim, W. B. (2010) · Journal of Korean Endocrine Society · doi:10.3803/jkes.2010.25.1.28
Development The DREAM Protein Is Associated with Thyroid Enlargement and Nodular
de Escobar, G. M., Naranjo, J. R., Rivas, M., Torres, B., Calı̀, G., Ferrara, A. M., & Terracciano, D. (2009)
Expression of hypoxia-inducible factor 1a in thyroid carcinomas
Burrows, N., Resch, J., Cowen, R. L., von Wasielewski, R., Hoang-vu, C., West, C., Williams, K. J., & Brabant, G. E. (2013)
Genetic and Epigenetic Alterations in the MAP Kinase and PI3K/Akt Pathways in Thyroid Cancer
Xing, M., & Ladenson, P. W. (2011) · CRC Press eBooks · doi:10.1201/b10945-4
Radioiodine-Refractory Thyroid Cancer: Restoring Response to Radioiodine Therapy
Fish, S. A., & Fagin, J. A. (2016) · Springer eBooks · doi:10.1007/978-1-4939-3314-3_68
Self-citationAndreghetto, F. M., Klingbeil, M. F. G., Soares, R. M., Sitnik, R., dos Santos, D., Júnior, P., Mathor, M. B., Nunes, F. D., & Severino, P. (2011)
Andreghetto, F. M., Klingbeil, M. F. G., Soares, R. M., Sitnik, R., dos Santos, D., Júnior, P., Mathor, M. B., Nunes, F. D., & Severino, P. (2011)
Kashat, L. (2013) · TSpace (University of Toronto)
Research ethics dilemmas in thyroid disease
Rosenthal, M. S., Angelos, P., & Schweppe, R. E. (2018) · Current Opinion in Endocrinology Diabetes and Obesity · doi:10.1097/med.0000000000000429
Self-citationDisease Models and Imaging Techniques Used in Pre-Clinical Studies - Endocrine Models
Serim, B. D., & Kula, M. (2019) · Nuclear Medicine Seminars · doi:10.4274/nts.galenos.2019.0011
Gretarsson, S., Nygren, A., Rosendahl, A. H., Mylona, N., Kjellén, E., Jin, Y., Paulsson, K. M., Borg, Å., Brun, E., Tennvall, J., et al. (2020) · Acta Oto-Laryngologica · doi:10.1080/00016489.2019.1704055
Somatic Genomic Changes in the Formation of Differentiated Thyroid Carcinoma
Vidinov, K. N., Dodova, R. I., Dimitrova, I., Mitkova, A. V., Shinkov, A. D., Kaneva, R. P., & Kovatcheva, R. D. (2020) · Acta Medica Bulgarica · doi:10.2478/amb-2020-0036
SID'07国際会議報告 : アクティブマトリクスデバイス(SID'07報告会)
小川, 康. (2007) · 電子情報通信学会技術研究報告. EID, 電子ディスプレイ
(2010) · Wiley eBooks · doi:10.1002/9780470649367.refs
Cote, G. J. (2010) · Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature · doi:10.3410/f.4812956.4725054
Data from IQGAP1 Plays an Important Role in the Invasiveness of Thyroid Cancer
Liu, Z., Liu, D., Bojdani, E., El‐Naggar, A. K., Vasko, V. V., & Xing, M. (2023) · doi:10.1158/1078-0432.c.6519284.v1
Janakiraman, M., Vakiani, E., Zeng, Z., Pratilas, C. A., Taylor, B. S., Chitale, D. A., Halilovic, E., Wilson, M., Huberman, K. H., Filho, J. C. R., et al. (2023) · doi:10.1158/0008-5472.22383576
Self-citationGule, M. K., Chen, Y., Sano, D., Frederick, M. J., Zhou, G., Zhao, M., Milas, Z. L., Galer, C. E., Henderson, Y. C., Jasser, S. A., et al. (2023) · doi:10.1158/1078-0432.c.6519536.v1
Data from IQGAP1 Plays an Important Role in the Invasiveness of Thyroid Cancer
Liu, Z., Liu, D., Bojdani, E., El‐Naggar, A. K., Vasko, V. V., & Xing, M. (2023) · doi:10.1158/1078-0432.c.6519284
Janakiraman, M., Vakiani, E., Zeng, Z., Pratilas, C. A., Taylor, B. S., Chitale, D. A., Halilovic, E., Wilson, M., Huberman, K. H., Filho, J. C. R., et al. (2023) · doi:10.1158/0008-5472.22383576.v1
Self-citationLimberg, J. N., Egan, C. E., Gray, K. D., Singh, M., Loewenstein, Z., Yang, Y., Riascos, M. C., Asadi, H. A., Safe, P., Eshaky, S. E., et al. (2023) · doi:10.1158/1541-7786.22528059.v1
Limberg, J. N., Egan, C. E., Gray, K. D., Singh, M., Loewenstein, Z., Yang, Y., Riascos, M. C., Asadi, H. A., Safe, P., Eshaky, S. E., et al. (2023) · doi:10.1158/1541-7786.22528059
Limberg, J. N., Egan, C. E., Gray, K. D., Singh, M., Loewenstein, Z., Yang, Y., Riascos, M. C., Asadi, H. A., Safe, P., Eshaky, S. E., et al. (2023) · doi:10.1158/1541-7786.22725440.v1
Limberg, J. N., Egan, C. E., Gray, K. D., Singh, M., Loewenstein, Z., Yang, Y., Riascos, M. C., Asadi, H. A., Safe, P., Eshaky, S. E., et al. (2023) · doi:10.1158/1541-7786.22725440
Shirai, Y.-T., Hoshi, N., Ward, J. M., Liu, H., Cachau, R. E., Lee, M. P., & Kimura, S. (2023) · Thyroid · doi:10.1089/thy.2023.0341
Establishment and validation of glioma patient-derived organoid models
Wang, A., Xie, X., Lu, H., Li, X., Huang, Y., & Wang, Z. (2024) · Glioma · doi:10.4103/glioma.glioma_5_24
Knockdown of ITGA2 Promotes Pyroptosis in Thyroid Cancer by Regulating the DNA Damage Response
Yan, L., Hua, D., Ying, R., Liu, X., Jiang, J. H., Liu, Z., & Feng, Y. (2025) · Frontiers in Bioscience-Landmark · doi:10.31083/fbl27946
Li, S., Zhong, R., Zhang, A., Li, J., Song, J., Ding, W., & Fang, Z. (2025) · BMC Pharmacology and Toxicology · doi:10.1186/s40360-025-00995-z
Angiogenesis regulation in hormone dependent breast- and ovarian cancer
Bendrik, C. (2011) · DiVA (Linkoping University)
Nakamura, T., Shibata, T., & Ito, K.-I. (2026) · PLoS ONE · doi:10.1371/journal.pone.0344276
Mejlachowicz, D. (2020) · theses.fr (ABES) · doi:10.70675/9628ed3ez2da1z4b55zab69z4a24dfdb174b
Sundaramurthi, S., Panneerselvam, G. S. D. A. V., & Panneerselvam, V. (2026) · IntechOpen eBooks · doi:10.5772/intechopen.1015841
BindingDB (2017) · UC San Diego · doi:10.7270/q2sf2z2g
Metadata
- Authors
- Schweppe, R. E., Klopper, J. P., Korch, C., Pugazhenthi, U., Benezra, M., Knauf, J. A., Fagin, J. A., Marlow, L. A., Copland, J. A., Smallridge, R. C., & Haugen, B. R.
- Affiliations
- University of Colorado Cancer Center (US)
- Memorial Sloan Kettering Cancer Center (US)
- Mayo Clinic in Florida (US)
- ORCID
- Venue
- The Journal of Clinical Endocrinology & Metabolism
- Publisher
- The Endocrine Society
- Published
- 2008-11-01
- Volume
- 93
- Issue
- 11
- Pages
- 4331-4341
- ISSN
- 0021-972X (ISSN-L)
- 1945-7197
- PMC
- PMC2582569
- OpenAlex
- W2027855079
- Cited by
- 563
- References
- 86
- Citations by year
- 2026: 3 · 2025: 7 · 2024: 11 · 2023: 28 · 2022: 9 · 2021: 21 · 2020: 22 · 2019: 27 · 2018: 33 · 2017: 33 · 2016: 38 · 2015: 40 · 2014: 29 · 2013: 47 · 2012: 54
- FWCI
- 38.71
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The Journal of Clinical Endocrinology & Metabolism (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Florida Department of Health
- Memorial Sloan-Kettering Cancer Center
- National Institutes of Health (CA-04-6934, P 30CA046934)
- University of Colorado Denver
- Cancer Center, University of Colorado (P30 CA046934, CA046934)
- National Cancer Institute (CA 046934, CA 50706, CA-72597, CA100560, R01CA100560, R01CA050706, R01CA072597, P30CA15083, P30CA015083, P30CA046934)
- Topics
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Thyroid Cancer Diagnosis and Treatment
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Identification and Quantification in Food
- Health Sciences › Medicine › Epidemiology › Cervical Cancer and HPV Research
- Keywords
- thyroid cancer cell lines, cell line contamination, cell line misidentification, short tandem repeat profiling, DNA fingerprinting, TTF-1, PAX8
- MeSH terms
- Colonic Neoplasms, DNA, Neoplasm, Female, Humans, Male, Melanoma, Mutation, Neoplasm Proteins, RNA, Messenger, Thyroid Neoplasms, Reproducibility of Results, Adenocarcinoma, Follicular, Cell Culture Techniques, Polymorphism, Single Nucleotide, Gene Expression Profiling, Cell Line, Tumor
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
