The DOI matches its Crossref record; 84 of the 86 references it cites were found in a registry or an index.
Deoxyribonucleic Acid Profiling Analysis of 40 Human Thyroid Cancer Cell Lines Reveals Cross-Contamination Resulting in Cell Line Redundancy and Misidentification
Open accessAbstract
CONTEXT: Cell lines derived from human cancers provide critical tools to study disease mechanisms and develop novel therapies. Recent reports indicate that up to 36% of cell lines are cross- contaminated. OBJECTIVE: We evaluated 40 reported thyroid cancer-derived cell lines using short tandem repeat and single nucleotide polymorphism array analysis. RESULTS: Only 23 of 40 cell lines tested have unique genetic profiles. The following groups of cell lines are likely derivatives of the same cell line: BHP5-16, BHP17-10, BHP14-9, and NPA87; BHP2-7, BHP10-3, BHP7-13, and TPC1; KAT5, KAT10, KAT4, KAT7, KAT50, KAK1, ARO81-1, and MRO87-1; and K1 and K2. The unique cell lines include BCPAP, KTC1, TT2609-C02, FTC133, ML1, WRO82-1, 8505C, SW1736, Cal-62, T235, T238, Uhth-104, ACT-1, HTh74, KAT18, TTA1, FRO81-2, HTh7, C643, BHT101, and KTC-2. The misidentified cell lines included the DRO90-1, which matched the melanoma-derived cell line, A-375. The ARO81-1 and its derivatives matched the HT-29 colon cancer cell line, and the NPA87 and its derivatives matched the M14/MDA-MB-435S melanoma cell line. TTF-1 and Pax-8 mRNA levels were determined in the unique cell lines. CONCLUSIONS: Many of these human cell lines have been widely used in the thyroid cancer field for the past 20 yr and are not only redundant, but not of thyroid origin. These results emphasize the importance of cell line integrity, and provide the short tandem repeat profiles for a panel of thyroid cancer cell lines that can be used as a reference for comparison of cell lines from other laboratories.
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In the citing paper
“Similarly, Schweppe and colleagues showed that nearly 50% of thyroid cell lines were misidentified [13].”
From Genetic Insights into the Economic Toll of Cell Line Misidentification: A Comprehensive Review (2026) · cites this paper as [13]
Experimental — accuracy not yet measuredIn the citing paper
“Cell lines were authenticated by short tandem repeat (STR) DNA profiling, and matched with their respective STR profiles as shown in previously published studies, and/or in the European Collection for Cell Cultures (ECACC) and the German Collection of Microorganisms and Cell Cultures (DSMZ) databases at all loci tested in common [48].”
From Restoration of p53 using the novel MDM2-p53 antagonist APG115 suppresses dedifferentiated papillary thyroid cancer cells (2017) · cites this paper as [48]
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Cites
2 retracted
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RetractedMelillo, R. M., Castellone, M. D., Guarino, V., Falco, V. D., Cirafici, A. M., Salvatore, G., Caiazzo, F., Basolo, F., Giannini, R., Kruhøffer, M., et al. (2005) · Journal of Clinical Investigation · doi:10.1172/jci22758 · Cited by 298
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Self-citationNikiforova, M. N., Kimura, E. T., Gandhi, M., Biddinger, P. W., Knauf, J. A., Basolo, F., Zhu, Z., Giannini, R., Salvatore, G., Fusco, A., et al. (2003) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2003-030838 · Cited by 1,036
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Molecular characterization of human prostate carcinoma cell lines
van Bokhoven, A., Varella‐Garcia, M., Korch, C. T., Johannes, W. U., Smith, E. E., Miller, H. L., Nordeen, S. K., Miller, G. J., & Lucia, M. S. (2003) · The Prostate · doi:10.1002/pros.10290 · Cited by 635
Self-citationClinical Implication of Hot SpotBRAFMutation, V599E, in Papillary Thyroid Cancers
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Short tandem repeat profiling provides an international reference standard for human cell lines
Masters, J. R., Thomson, J. A., Daly-Burns, B., Reid, Y. A., Dirks, W. G., Packer, P., Toji, L. H., Ohno, T., Tanabe, H., Arlett, C. F., et al. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.121616198 · Cited by 478
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Ricarte‐Filho, J. C., Ryder, M. M., Chitale, D. A., Rivera, M., Heguy, A., Ladanyi, M., Janakiraman, M., Solit, D. B., Knauf, J. A., Tuttle, R. M., et al. (2009) · Cancer Research · doi:10.1158/0008-5472.can-09-0727 · Cited by 532
Self-citationCheck your cultures! A list of cross‐contaminated or misidentified cell lines
Capes‐Davis, A., Theodosopoulos, G., Atkin, I., Drexler, H. G., Kohara, A., MacLeod, R. A. F., Masters, J. R., Nakamura, Y., Reid, Y. A., Reddel, R. R., et al. (2010) · International Journal of Cancer · doi:10.1002/ijc.25242 · Cited by 520
Anaplastic Thyroid Carcinoma: Pathogenesis and Emerging Therapies
Smallridge, R. C., & Copland, J. A. (2010) · Clinical Oncology · doi:10.1016/j.clon.2010.03.013 · Cited by 480
Self-citationAnaplastic thyroid cancer: molecular pathogenesis and emerging therapies
Smallridge, R. C., Marlow, L. A., & Copland, J. A. (2008) · Endocrine Related Cancer · doi:10.1677/erc-08-0154 · Cited by 396
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Metadata
- Authors
- Schweppe, R. E., Klopper, J. P., Korch, C., Pugazhenthi, U., Benezra, M., Knauf, J. A., Fagin, J. A., Marlow, L. A., Copland, J. A., Smallridge, R. C., & Haugen, B. R.
- Affiliations
- University of Colorado Cancer Center (US)
- Memorial Sloan Kettering Cancer Center (US)
- Mayo Clinic in Florida (US)
- ORCID
- Venue
- The Journal of Clinical Endocrinology & Metabolism
- Publisher
- The Endocrine Society
- Published
- 2008-11-01
- Volume
- 93
- Issue
- 11
- Pages
- 4331-4341
- ISSN
- 0021-972X (ISSN-L)
- 1945-7197
- PMC
- PMC2582569
- OpenAlex
- W2027855079
- Cited by
- 563
- References
- 86
- Citations by year
- 2026: 3 · 2025: 7 · 2024: 11 · 2023: 28 · 2022: 9 · 2021: 21 · 2020: 22 · 2019: 27 · 2018: 33 · 2017: 33 · 2016: 38 · 2015: 40 · 2014: 29 · 2013: 47 · 2012: 54
- FWCI
- 38.71
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The Journal of Clinical Endocrinology & Metabolism (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Florida Department of Health
- Memorial Sloan-Kettering Cancer Center
- National Institutes of Health (CA-04-6934, P 30CA046934)
- University of Colorado Denver
- Cancer Center, University of Colorado (P30 CA046934, CA046934)
- National Cancer Institute (CA 046934, CA 50706, CA-72597, CA100560, R01CA100560, R01CA050706, R01CA072597, P30CA15083, P30CA015083, P30CA046934)
- Topics
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Thyroid Cancer Diagnosis and Treatment
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Identification and Quantification in Food
- Health Sciences › Medicine › Epidemiology › Cervical Cancer and HPV Research
- Keywords
- thyroid cancer cell lines, cell line contamination, cell line misidentification, short tandem repeat profiling, DNA fingerprinting, TTF-1, PAX8
- MeSH terms
- Colonic Neoplasms, DNA, Neoplasm, Female, Humans, Male, Melanoma, Mutation, Neoplasm Proteins, RNA, Messenger, Thyroid Neoplasms, Reproducibility of Results, Adenocarcinoma, Follicular, Cell Culture Techniques, Polymorphism, Single Nucleotide, Gene Expression Profiling, Cell Line, Tumor
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