The DOI matches its Crossref record; 39 of the 39 references it cites were found in a registry or an index.
Steroidogenic Enzyme Gene Expression in the Human Heart
Abstract
Corticosteroids have specific effects on cardiac structure and function mediated by mineralocorticoid (MR) and glucocorticoid (GR) receptors. Aldosterone and corticosterone are synthesized in the rat heart. To see whether they might also be synthesized in the human cardiovascular system, we examined the expression of genes for steroidogenic enzymes as well as genes for GR, MR, and 11-hydroxysteroid dehydrogenase (11-HSD2; which maintains the specificity of MR). Human samples were from left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart. Using RT-PCR, messenger ribonucleic acids encoding cholesterol side-chain cleavage enzyme (CYP11A), 3beta-HSD2, 21-hydroxylase (CYP21), 11beta-hydroxylase (CYP11B1), GR, MR, and 11-HSD2 were detected in all samples with the exception of the ventricles, which did not express CYP11B1. Aldosterone synthase (CYP11B2) messenger ribonucleic acid was detected in the aorta and fetal heart, but not in any region of the adult heart, and 17alpha-hydroxylase (CYP17) was not detected in any cardiac sample. Levels of steroidogenic enzyme gene expression were typically 0.1% those in the adrenal gland. These findings are consistent with autocrine or paracrine roles for corticosterone and deoxycorticosterone, but not cortisol or aldosterone, in the normal adult human heart.
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“In normal adult adrenal glands, CYP21A2 immunoreactivity is detected in all three cortical layers, particularly the zonae glomerulosa and reticularis, with variegated expression in the zona fasciculata.”
From Congenital Adrenal Hyperplasia—Current Insights in Pathophysiology, Diagnostics, and Management (2022) · cites this paper as [160]
In the citing paper
“The immunoreactivity is more intense in adrenals from patients with Cushing disease and at sites of regeneration in normal adrenal glands (160).”
From Congenital Adrenal Hyperplasia—Current Insights in Pathophysiology, Diagnostics, and Management (2022) · cites this paper as [160]
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Cites
The Effect of Spironolactone on Morbidity and Mortality in Patients with Severe Heart Failure
Pitt, B., Zannad, F., Remme, W. J., Cody, R., Castaigne, A., Perez, A., Palensky, J., & Wittes, J. (1999) · New England Journal of Medicine · doi:10.1056/nejm199909023411001 · Cited by 9,214
Myocardial Production of Aldosterone and Corticosterone in the Rat
Silvestre, J.-S., Robert, V., Heymes, C., Aupetit-Faisant, B., Mouas, C., Moalic, J.-M., Swynghedauw, B., & Delcayre, C. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.9.4883 · Cited by 446
Mineralocorticoids, hypertension, and cardiac fibrosis.
Young, M., Fullerton, M., Dilley, R., & Funder, J. (1994) · Journal of Clinical Investigation · doi:10.1172/jci117269 · Cited by 437
Brilla, C. G., Matsubara, L. S., & Weber, K. T. (1993) · Journal of Molecular and Cellular Cardiology · doi:10.1006/jmcc.1993.1066 · Cited by 425
Silvestre, J.-S., Heymes, C., Oubénaïssa, A., Robert, V., Aupetit-Faisant, B., Carayon, A., Swynghedauw, B., & Delcayre, C. (1999) · Circulation · doi:10.1161/01.cir.99.20.2694 · Cited by 380
Atrial natriuretic factor receptors and signal transduction mechanisms.
Anand-Srivastava, M. B., & Trachte, G. J. (1993) · Pharmacological Reviews · doi:10.1016/s0031-6997(25)00429-6 · Cited by 367
Hatakeyama, H., Miyamori, I., Fujita, T., Takeda, Y., Takeda, R., & Yamamoto, H. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)51084-5 · Cited by 316
Immunohistochemical and biochemical evidence for a cardiovascular mineralocorticoid receptor.
Lombès, M., Oblin, M. E., Gasc, J. M., Baulieu, E. E., Farman, N., & Bonvalet, J. P. (1992) · Circulation Research · doi:10.1161/01.res.71.3.503 · Cited by 309
Lombès, M., Alfaidy, N., Eugene, E., Lessana, A., Farman, N., & Bonvalet, J.-P. (1995) · Circulation · doi:10.1161/01.cir.92.2.175 · Cited by 292
Disorders of Aldosterone Biosynthesis and Action
Epstein, F. H., & White, P. C. (1994) · New England Journal of Medicine · doi:10.1056/nejm199407283310408 · Cited by 225
Cited by
Overview of steroidogenic enzymes in the pathway from cholesterol to active steroid hormones
Payne, A. H., & Hales, D. B. (2004) · Endocrine Reviews · doi:10.1210/er.2003-0030 · Cited by 1,766
Congenital Adrenal Hyperplasia—Current Insights in Pathophysiology, Diagnostics, and Management
Claahsen - van der Grinten, H. L., Speiser, P. W., Ahmed, S. F., Arlt, W., Auchus, R. J., Falhammar, H., Flück, C. E., Guasti, L., Huebner, A., Kortmann, B. B. M., et al. (2022) · Endocrine Reviews · doi:10.1210/endrev/bnab016 · Cited by 556
Glucocorticoids and cardiovascular disease
Walker, B. R. (2007) · European Journal of Endocrinology · doi:10.1530/eje-07-0455 · Cited by 529
Jaffe, I. Z., & Mendelsohn, M. E. (2005) · Circulation Research · doi:10.1161/01.res.0000159937.05502.d1 · Cited by 358
The new biology of aldosterone
Connell, J. M. C., & Davies, E. (2005) · Journal of Endocrinology · doi:10.1677/joe.1.06017 · Cited by 356
Metadata
- Authors
- Kayes-Wandover, K. M.
- Venue
- Journal of Clinical Endocrinology & Metabolism
- Publisher
- The Endocrine Society
- Published
- 2000-07-01
- Volume
- 85
- Issue
- 7
- Pages
- 2519-2525
- ISSN
- 0021-972X (ISSN-L)
- OpenAlex
- W2084491739
- Cited by
- 174
- References
- 39
- Citations by year
- 2026: 1 · 2025: 3 · 2023: 1 · 2022: 5 · 2021: 3 · 2020: 4 · 2019: 4 · 2018: 4 · 2017: 6 · 2016: 7 · 2015: 6 · 2014: 7 · 2013: 7 · 2012: 11
- FWCI
- 6.73
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- The Journal of Clinical Endocrinology & Metabolism (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of Diabetes and Digestive and Kidney Diseases (R01DK054408)
- Topics
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Hormonal Regulation and Hypertension
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Estrogen and related hormone effects
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adrenal Hormones and Disorders
- Keywords
- steroidogenic enzyme gene expression, human heart, mineralocorticoid receptor, glucocorticoid receptor, 11β-hydroxysteroid dehydrogenase type 2, aldosterone synthase, CYP11B2, CYP11A, CYP21, CYP11B1, CYP17, corticosterone, deoxycorticosterone, fetal heart
- MeSH terms
- Aldosterone, Animals, Autoradiography, Cholesterol, DNA, Humans, Hydrocortisone, Hydroxysteroid Dehydrogenases, Myocardium, Receptors, Glucocorticoid, RNA, Messenger, Steroids, Gene Expression Regulation, Enzymologic, DNA Primers, Receptors, Mineralocorticoid, Reverse Transcriptase Polymerase Chain Reaction, 11-beta-Hydroxysteroid Dehydrogenases, Rats
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- Kayes-Wandover, K. M. (2000). Steroidogenic Enzyme Gene Expression in the Human Heart. Journal of Clinical Endocrinology & Metabolism. https://doi.org/10.1210/jc.85.7.2519
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
