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Cited by 280Pharmacogenetics and Drug MetabolismEstrogen and related hormone effectsSteroid Chemistry and Biochemistry

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Interactions Between Estrogens and Catechol Amines III. Studies on the Methylation of Catechol Estrogens, Catechol Amines and other Catechols by the Catechol-O-Methyltransferase1 of Human Liver

BALL, PETER, KNUPPEN, RUDOLF, HAUPT, MARGITTA, BREUER, HEINZ·1972·The Journal of Clinical Endocrinology & Metabolism
DOI 10.1210/jcem-34-4-736
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Abstract

The substrate specificity of the catechol-O-methyltransferase, purified from human liver 380-fold, has been tested with a variety of compounds. Catechol estrogens, as well as catechol amines are methylated to their corresponding monomethyl ethers. The relation of the rates of methylation of catechol estrogens and catechol amines depended on enzyme and substrate concentration; under standard assay conditions, 2-hydroxy-17β-estradiol was the preferred substrate for the enzyme, as compared with epinephrine and other catechols tested. Km-values for 2-hydroxy-17β-estradiol were 15 μm, for 2-hydroxy-estrone 20 μm, for 2-hydroxy-estriol 25 μm and for-hydroxy-estrone 20 μm. For epinephrine and norepinephrine, Km-values of 300 μm and 200 μm, respectively, were obtained. The ratio of formation of the isomeric monomethyl ethers of catechol estrogens varied between 1.3 and 95, depending on the substrate incubated. On the basis of kinetic studies with different enzyme preparations, different inhibitors and different concentrations of the substrates, it is concluded that both catechol estrogens and catechol amines are methylated by the same Catechol-O-methyltransferase. The enzymic methylation of epinephrine was inhibited competitively by 2- and 4-hydroxylated estrogens; the Ki-values were 7.0 μm for 2-hydroxy-17β-estradiol, 8.5 μm for 2-hydroxy-estrone, 12.2 μm for 2-hydroxy-estriol, 50 μm for 4-hydroxy-estrone and, for comparison, 154 μm for the addition of norepinephrine. A non-competitive inhibition was observed with the corresponding isomeric monomethyl ethers. The extent of inhibition not only depended on the relative concentrations of substrates and inhibitors, but also on the concentrations of MgCl2S-adenosylmethionine and enzyme. The results reported here indicate that the enzymic methylation and biological inactivation of neurotransmitters is strongly inhibited by 2-hydroxy-estrogens. These findings are of possible clinical relevance with respect to the regulation of blood pressure under normal and pathological conditions.

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Metadata

Authors
BALL, P., KNUPPEN, R., HAUPT, M., & BREUER, H.
Affiliations
University of Bonn (DE)
Venue
The Journal of Clinical Endocrinology & Metabolism
Publisher
The Endocrine Society
Published
1972-04
Volume
34
Issue
4
Pages
736-746
ISSN
  • 0021-972X (ISSN-L)
  • 1945-7197
DOI
10.1210/jcem-34-4-736
OpenAlex
W2010928336
Cited by
280
References
0
Citations by year
2025: 1 · 2023: 2 · 2022: 1 · 2021: 1 · 2020: 1 · 2019: 2 · 2018: 3 · 2017: 2 · 2016: 1 · 2015: 1 · 2014: 3 · 2013: 2 · 2012: 2
FWCI
6.20
Type
journal article
Language
en
Open access
closed
Locations
  • The Journal of Clinical Endocrinology & Metabolism (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Cancer Institute
Topics
  • Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmacology › Pharmacogenetics and Drug Metabolism
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Estrogen and related hormone effects
  • Physical Sciences › Chemistry › Organic Chemistry › Steroid Chemistry and Biochemistry
Keywords
catechol estrogens, epinephrine, norepinephrine, methylation, 2-hydroxyestrone, 4-hydroxyestrone, monomethyl ether, substrate specificity, enzyme kinetics, competitive inhibition, non-competitive inhibition, human liver
MeSH terms
Adult, Carbon Isotopes, Catecholamines, Catechols, Chemistry, Chlorides, Cysteine, Dopamine, Epinephrine, Estradiol, Estriol, Estrogens, Estrone, Humans, Kinetics, Liver, Magnesium, Male, Mandelic Acids, Methylation, Methyltransferases, Norepinephrine, S-Adenosylmethionine, Time Factors, Chemical Phenomena

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