The DOI matches its Crossref record.
A Radioimmunoassay for Cortisol in Plasma and Urine
Abstract
ABSTRACT A radioligand assay for the measurement of cortisol in plasma and urine has been developed. Antibodies raised against cortisol-21-hemisuccinate conjugated to bovine serum albumin had a high affinity for cortisol (K = 2 × 109 1/mole). A standard curve was established with a useful range from 0.10–4.0 ng. Plasma cortisol is readily measured by directly assaying small aliquots of a methylene chloride extract of plasma. Urine free cortisol was assayed after a single TLC of a methylene chloride extract of urine. Intraassay variability of both urine and plasma cortisol determinations was 10%; interassay variability was 20–26%. This method is particularly useful for measurement of 24-hr urine free cortisol.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Adrenarche: a survey of rodents, domestic animals and primates
CUTLER, G. B., GLENN, M., BUSH, M., HODGEN, G. D., GRAHAM, C. E., & LORIAUX, D. L. (1978) · Endocrinology · doi:10.1210/endo-103-6-2112 · Cited by 321
Recent advances in cortisol sensing technologies for point-of-care application
Kaushik, A., Vasudev, A., Arya, S. K., Pasha, S. K., & Bhansali, S. (2014) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2013.09.060 · Cited by 316
Use of saliva for monitoring unbound free cortisol levels in serum
Teruhisa, U., Ryoji, H., Taisuke, I., Tatsuya, S., Fumihiro, M., & Tatsuo, S. (1981) · Clinica Chimica Acta · doi:10.1016/0009-8981(81)90353-3 · Cited by 314
Determination of cortisol in serum, saliva and urine
Turpeinen, U., & Hämäläinen, E. (2013) · Best Practice & Research Clinical Endocrinology & Metabolism · doi:10.1016/j.beem.2013.10.008 · Cited by 214
Glucocorticoid hormone resistance during primate evolution: receptor-mediated mechanisms
Chrousos, G. P., Renquist, D., Brandon, D., Eil, C., Pugeat, M., Vigersky, R., Cutler, G. B., Loriaux, D. L., & Lipsett, M. B. (1982) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.79.6.2036 · Cited by 179
Self-citation
Metadata
- Authors
- RUDER, H. J., GUY, R. L., & LIPSETT, M. B.
- Venue
- The Journal of Clinical Endocrinology & Metabolism
- Publisher
- The Endocrine Society
- Published
- 1972-08
- Volume
- 35
- Issue
- 2
- Pages
- 219-224
- ISSN
- 0021-972X (ISSN-L)
- 1945-7197
- OpenAlex
- W2136165959
- Cited by
- 329
- References
- 0
- FWCI
- 9.59
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: A Radioimmunoassay for Cortisol in Plasma and Urine. https://app.cochat.ai/papers/10.1210/jcem-35-2-219
- The paper
- RUDER, H. J., GUY, R. L., & LIPSETT, M. B. (1972). A Radioimmunoassay for Cortisol in Plasma and Urine. The Journal of Clinical Endocrinology & Metabolism. https://doi.org/10.1210/jcem-35-2-219
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
