Cited by 314Adrenal Hormones and DisordersStress Responses and CortisolClinical Laboratory Practices and Quality Control

Use of saliva for monitoring unbound free cortisol levels in serum

Teruhisa, Umeda, Ryoji, Hiramatsu, Taisuke, Iwaoka, Tatsuya, Shimada, Fumihiro, Miura, Tatsuo, Sato·1981·Clinica Chimica Acta

Abstract

In order to verify the clinical usefulness of saliva in assessing the adrenocortical function, we measured saliva cortisol levels by a radioimmunoassay after extraction with dichloromethane, and compared the results with the levels of serum unbound cortisol determined by the method of equilibrium dialysis. Paired samples of saliva and serum were obtained from 10 healthy male volunteers. Morning levels of saliva cortisol and serum unbound cortisol were 0.99 +/- 0.42 and 1.56 +/- 0.54 microgram/100 ml, respectively, where serum total cortisol was 19.28 +/- 3.56 microgram/100 ml. A well-defined diurnal rhythm and a greater response to ACTH stimulation were observed in saliva cortisol than in serum total cortisol. Kinetic study of injected cortisol revealed almost identical values for the compartments of saliva cortisol and serum unbound cortisol. The correlation coefficient (r) between the levels of saliva cortisol and serum unbound cortisol was 0.893 (p less than 0.001, n = 150). From these results we concluded that the measurement of saliva cortisol can be used sufficiently to monitor unbound free concentrations in serum.

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Crossref · 10.1016/0009-8981(81)90353-3

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Authors
Teruhisa, U., Ryoji, H., Taisuke, I., Tatsuya, S., Fumihiro, M., & Tatsuo, S.
Affiliations
Kumamoto University (JP)
Corresponding author
Teruhisa, Umeda
Venue
Clinica Chimica Acta
Publisher
Elsevier BV
Published
1981-03
Volume
110
Issue
2-3
Pages
245-253
ISSN
0009-8981 (ISSN-L)
OpenAlex
W2006949121
Cited by
314
References
23
Citations by year
2026: 1 · 2025: 3 · 2024: 7 · 2023: 20 · 2022: 5 · 2021: 7 · 2020: 11 · 2019: 10 · 2018: 8 · 2017: 4 · 2016: 8 · 2015: 12 · 2014: 10 · 2013: 13 · 2012: 14
FWCI
5.21
Type
journal article
Language
en
Open access
closed
Locations
  • Clinica Chimica Acta (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adrenal Hormones and Disorders
  • Life Sciences › Neuroscience › Behavioral Neuroscience › Stress Responses and Cortisol
  • Health Sciences › Medicine › Physiology › Clinical Laboratory Practices and Quality Control
Keywords
salivary cortisol, serum cortisol, adrenocortical function, diurnal cycle, ACTH stimulation test, equilibrium dialysis, radioimmunoassay
MeSH terms
Adrenal Cortex, Adrenocorticotropic Hormone, Adult, Circadian Rhythm, Dialysis, Humans, Hydrocortisone, Kinetics, Male, Radioimmunoassay, Saliva

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