Measurement of Norepinephrine and Epinephrine in Small Volumes of Human Plasma by a Single Isotope Derivative Method: Response to the Upright Posture
Abstract
A single-isotope modification of the double-isotope derivative method for the measurement of norepinephrine and epinephrine, employing S-adenosylmethionine (3H-methyl) rather than S-andenosylmethionine (14C-methyl), was found to be sufficiently sensitive (to less than 10 pg/ml) and reproducible (interassay coefficient of variation of 3.5% for norepinephrine and 8.5% for epinephrine) for the measurement of these catecholamines in 1.0 ml of human plasma obtained from supine, resting subjects. In 8 normal subjects the mean (±SE) plasma norepinephrine concentration rose from 182 ± 21 pg/ml in the resting, supine position to 425 ± 28 pg/ml (p < 0.001) after 5 mins in the standing position. Corresponding supine and standing mean plasma epinephrine concentrations were 44 ± 11 pg/ml and 73 ± 17 pg/ml (p < 0.02) respectively. Supine plasma norepinephrine and epinephrine concentrations measured in 18 subjects with this method were quite comparable to those reported with the parent double-isotope method.
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Mean plasma epinephrine concentrations rise nearly 2-fold during quiet standing, ~3-fold during cigarette smoking, from 2-13-fold during mild to heavy exercise, and 50-fold during insulin-induced hypoglycemia.
“mean plasma epinephrine concentrations rise nearly 2-fold during quiet standing (6), ~3-fold during cigarette smoking (26), from 2–13-fold during mild to heavy exercise (27), and 50fold during insulin-induced hypoglycemia (28).”
From Epinephrine plasma metabolic clearance rates and physiologic thresholds for metabolic and hemodynamic actions in man. (1980) · cites this paper as [6]
Plasma norepinephrine and epinephrine concentrations were measured by a single isotope derivative method.
“Plasma norepinephrine and epinephrine concentrations were measured by a single isotope derivative method (6, 7).”
From Epinephrine plasma metabolic clearance rates and physiologic thresholds for metabolic and hemodynamic actions in man. (1980) · cites this paper as [6]
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- Authors
- CRYER, P. E., SANTIAGO, J. V., & SHAH, S.
- Affiliations
- Washington University in St. Louis (US)
- Venue
- The Journal of Clinical Endocrinology & Metabolism
- Publisher
- The Endocrine Society
- Published
- 1974-12
- Volume
- 39
- Issue
- 6
- Pages
- 1025-1029
- ISSN
- 0021-972X (ISSN-L)
- 1945-7197
- OpenAlex
- W2150416141
- Cited by
- 155
- References
- 0
- Citations by year
- 2025: 1 · 2018: 1 · 2015: 1 · 2014: 1 · 2013: 1
- FWCI
- 5.77
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- The Journal of Clinical Endocrinology & Metabolism (publishedVersion)
- PubMed (publishedVersion)
- Funders
- NIH Clinical Center
- Topics
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adrenal Hormones and Disorders
- Physical Sciences › Chemistry › Analytical Chemistry › Analytical Methods in Pharmaceuticals
- Health Sciences › Medicine › Physiology › Clinical Laboratory Practices and Quality Control
- Keywords
- norepinephrine, epinephrine, catecholamines, S-adenosylmethionine, upright posture, standing posture, supine position, sympathetic response
- MeSH terms
- Adolescent, Adult, Aged, Animals, Carbon Radioisotopes, Catechol O-Methyltransferase, Epinephrine, Female, Humans, Isotope Labeling, Liver, Male, Methods, Middle Aged, Monoamine Oxidase, Norepinephrine, Posture, S-Adenosylmethionine, Tritium, Rats
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