CoChat
Sign Up
Cited by 371Thyroid Disorders and TreatmentsClinical Laboratory Practices and Quality ControlGrowth Hormone and Insulin-like Growth Factors

The DOI matches its Crossref record; 16 of the 16 references it cites were found in a registry or an index.

Applications of a New Chemiluminometric Thyrotropin Assay to Subnormal Measurement*

SPENCER, C. A., LoPRESTI, J. S., PATEL, A., GUTTLER, R. B., EIGEN, A., SHEN, D., GRAY, D., NICOLOFF, J. T.·1990·The Journal of Clinical Endocrinology & Metabolism
DOI 10.1210/jcem-70-2-453
Open this paper in CoChat — free account

Abstract

A new immunochemiluminometric TSH assay (ICMA) was shown to offer improved analytical (+2 SD of zero) and functional (20% interassay coefficient of variation) sensitivity [0.003 vs 0.045 +/- 0.005 (+/- SE; range, 0.01-0.07); 0.018 vs. 0.23 +/- 0.02 (range, 0.10-0.35, mU/L); analytical vs. functional sensitivity limit for the ICMA vs. 10 other TSH immunometric assays, respectively]. The ICMA was used to study the physiological relationship between serum TSH and free T4 [as reflected by free T4 index (FT4I)] values at both steady state and 14 days after acute pharmacological T4 administration (3 mg oral T4 load plus 0.3 mg daily). At steady state, an inverse log/linear relationship was found between serum TSH and FT4I values (log TSH = 2.56 - 0.022 FT4I; r = 0.84; P less than 0.001). Ten to 14 days after acute T4 suppression in 5 euthyroid subjects, serum TSH/FT4I levels had plateaued after decreasing in parallel to the slope of the steady state relationship, suggesting that the degree of T4 suppression of TSH can be predicted from an individual's pituitary TSH/free T4 set-point and the magnitude of the serum T4 elevation achieved. Ambulatory and hospitalized patient sera, previously identified as having low (less than 0.1 mU/L) TSH levels by a less sensitive assay, were restudied by the TSH ICMA. Normal TSH values ranged from 0.39-4.6 mU/L, whereas the majority of hyperthyroid patients [52 of 54 (96% ambulatory) and 22 of 23 (96%, hospitalized)] had undetectable (less than 0.005 mU/L), basal TSH levels and absent TRH stimulated TSH responses. In contrast, most (32 of 37; 86%) of hospitalized nonhyperthyroid patients with low (less than 0.1 mU/L) TSH values due to nonthyroidal illness or glucocorticoid treatment had detectable (greater than 0.01 mU/L) basal and TRH stimulated TSH levels. The positive relationship between basal and TRH-stimulated TSH levels was shown to extend down to the detectability limit of the assay (0.005 mU/L), which further supported the authenticity of the subnormal TSH ICMA measurements. The new TSH ICMA is considered to represent the first of a third generation of clinical TSH assays, since it has a functional (interassay) sensitivity that is 2 orders of magnitude greater than that of typical first generation TSH RIAs and 1 order of magnitude greater than current second generation TSH immunometric methods. Such third generation TSH assays will facilitate both the optimization of T4 therapy as well as the diagnosis of hyperthyroidism in hospitalized patients with nonthyroidal illness.

Overview

Integrity signals

Registry

Crossref · 10.1210/jcem-70-2-453

Cites

16 of 16 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Show all 16 references

Cited by

Sorted by citations

Items 101–200 of 370

Related

See all

Metadata

Authors
SPENCER, C. A., LoPRESTI, J. S., PATEL, A., GUTTLER, R. B., EIGEN, A., SHEN, D., GRAY, D., & NICOLOFF, J. T.
Affiliations
University of Southern California (US)
ORCID
Venue
The Journal of Clinical Endocrinology & Metabolism
Publisher
The Endocrine Society
Published
1990-02
Volume
70
Issue
2
Pages
453-460
ISSN
  • 0021-972X (ISSN-L)
  • 1945-7197
DOI
10.1210/jcem-70-2-453
OpenAlex
W2099864259
Cited by
371
References
16
Citations by year
2026: 2 · 2025: 6 · 2024: 6 · 2023: 9 · 2022: 10 · 2021: 16 · 2020: 16 · 2019: 5 · 2018: 13 · 2017: 4 · 2016: 15 · 2015: 8 · 2014: 9 · 2013: 15 · 2012: 13
FWCI
12.89
Type
journal article
Language
en
Open access
closed
Locations
  • The Journal of Clinical Endocrinology & Metabolism (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • University of Southern California
  • National Institutes of Health (5r01dk011727-24)
  • National Institute of Diabetes and Digestive and Kidney Diseases (DK11727)
  • National Center for Research Resources (M01 RR-43)
Topics
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Thyroid Disorders and Treatments
  • Health Sciences › Health Professions › Medical Laboratory Technology › Clinical Laboratory Practices and Quality Control
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Growth Hormone and Insulin-like Growth Factors
Keywords
thyroid-stimulating hormone, low TSH, free T4 index, hyperthyroidism, low T3 syndrome, TRH stimulation test, thyroid function tests, assay sensitivity
MeSH terms
Adult, Autoanalysis, Drug Administration Schedule, Female, Humans, Hyperthyroidism, Immunoassay, Luminescent Measurements, Male, Thyrotropin, Thyrotropin-Releasing Hormone, Thyroxine

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review