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Cited by 165

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Characterization of an Organic Anion-Transporting Polypeptide (OATP-B) in Human Placenta

St-Pierre, M. V., Hagenbuch, B., Ugele, B., Meier, P. J., Stallmach, T.·2002·The Journal of Clinical Endocrinology & Metabolism
DOI 10.1210/jcem.87.4.8431
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Abstract

Organic anion-transporting polypeptides (OATPs) are a family of multispecific carriers that mediate the sodium-independent transport of steroid hormone and conjugates, drugs, and numerous anionic endogenous substrates. We investigated whether members of the OATP gene family could mediate fetal-maternal transfer of anionic steroid conjugates in the human placenta. OATP-B (gene symbol SLC21A9) was isolated from a placenta cDNA library. An antiserum to OATP-B detected an 85-kDa protein in basal but not apical syncytiotrophoblast membranes. Immunohistochemistry of first-, second-, and third-trimester placenta showed staining in the cytotrophoblast membranes and at the basal surface of the syncytiotrophoblast. Trophoblasts that reacted with an antibody to Ki-67, a proliferation-associated antigen, expressed lower levels of OATP-B. OATP-B mRNA levels were measured in isolated trophoblasts under culture conditions that promoted syncytia formation. Real-time quantitative PCR estimated an 8-fold increase in OATP-B expression on differentiation to syncytia. The uptake of [(3)H]estrone-3-sulfate, a substrate for OATP-B, was measured in basal syncytiotrophoblast membrane vesicles. Transport was saturable and partially inhibited by pregnenolone sulfate, a progesterone precursor. Pregnenolone sulfate also partially inhibited OATP-B-mediated transport of estrone-3-sulfate in an oocyte expression system. These findings suggest a physiological role for OATP-B in the placental uptake of fetal-derived sulfated steroids.

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Metadata

Authors
St-Pierre, M. V., Hagenbuch, B., Ugele, B., Meier, P. J., & Stallmach, T.
Venue
The Journal of Clinical Endocrinology & Metabolism
Publisher
The Endocrine Society
Published
2002-04-01
Volume
87
Issue
4
Pages
1856-1863
ISSN
  • 0021-972X (ISSN-L)
  • 1945-7197
DOI
10.1210/jcem.87.4.8431
OpenAlex
W2170376158
Cited by
165
References
1
FWCI
3.84
Type
journal article
Language
en
Open access
closed

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CoChat. (2026). Citation check: Characterization of an Organic Anion-Transporting Polypeptide (OATP-B) in Human Placenta. https://app.cochat.ai/papers/10.1210/jcem.87.4.8431
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St-Pierre, M. V., Hagenbuch, B., Ugele, B., Meier, P. J., & Stallmach, T. (2002). Characterization of an Organic Anion-Transporting Polypeptide (OATP-B) in Human Placenta. The Journal of Clinical Endocrinology & Metabolism. https://doi.org/10.1210/jcem.87.4.8431

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