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Direct Effect of Glucocorticoids on Lipolysis in Adipocytes
Open accessAbstract
Hypercortisolemia and glucocorticoid treatment cause elevated level of circulating free fatty acids (FFAs). The basis of this phenomenon has long been linked to the effect of glucocorticoids permitting and enhancing the adipose lipolysis response to various hormones. In this study, we demonstrate that glucocorticoids directly stimulate lipolysis in rat primary adipocytes in a dose- and time-responsive manner; this lipolytic action was attenuated by treatment with the glucocorticoid antagonist RU486. Dexamethasone down-regulates mRNA and protein levels of cyclic-nucleotide phosphodiesterase 3B, thereby elevating cellular cAMP production and activating protein kinase A (PKA). On inhibition of PKA but not other kinases, the lipolysis response ceases. Furthermore, dexamethasone induces phosphorylation and down-regulation of perilipin, a lipid droplet-associating protein that modulates lipolysis; this effect is restored by RU486 or PKA inhibitor H89. Dexamethasone up-regulates mRNA and protein levels of hormone-sensitive lipase (HSL) and adipose triglyceride lipase; these effects, parallel to increased lipolysis, are attenuated by RU486 or actinomycin D. Phosphorylation at Ser-563 and Ser-660 residues of HSL and activity of cellular lipases are elevated on dexamethasone stimulation but abrogated by the coaddition of H89. However, dexamethasone does not induce HSL translocation to the lipid droplet surface in differentiated adipocytes. We show that elevated FFA concentration in plasma is associated with increased lipase activity and lipolysis in vivo in adipose tissues of dexamethasone-treated rats. Therefore, the lipolytic action of glucocorticoids liberates FFA efflux from adipocytes to the bloodstream, which could be a cellular basis of systemic FFA elevation in response to glucocorticoid challenge.
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“However, glucocorticoids enhance basal lipolysis in rodents,4, 23 which we did not observe in humans, highlighting species‐specific differences in the glucocorticoid regulation of lipolysis.”
From Acute physiological effects of glucocorticoids on fuel metabolism in humans are permissive but not direct (2017) · cites this paper as [4]
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“For example, in vitro studies in adipocytes have shown lipolytic rates to be increased,4 unchanged5 or decreased6 by glucocorticoids.”
From Acute physiological effects of glucocorticoids on fuel metabolism in humans are permissive but not direct (2017) · cites this paper as [4]
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“Additional genes outside the classic lipolytic pathway have been implicated in the lipolytic effects of glucocorticoids, such as phosphodiesterase 3b,4 pigment epithelium derived factor24 and angiopoietin‐like 4;25”
From Acute physiological effects of glucocorticoids on fuel metabolism in humans are permissive but not direct (2017) · cites this paper as [4]
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“Although previous studies have tested how glucocorticoids regulate the lipolytic pathway in rodents (by increasing ATGL and HSL),4, 23 this had not been tested in vivo in humans.”
From Acute physiological effects of glucocorticoids on fuel metabolism in humans are permissive but not direct (2017) · cites this paper as [4]
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“Although previous studies have tested how glucocorticoids regulate the lipolytic pathway in rodents (by increasing ATGL and HSL),4, 23 this had not been tested in vivo in humans.”
From Acute physiological effects of glucocorticoids on fuel metabolism in humans are permissive but not direct (2017) · cites this paper as [4]
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Cites
Mechanisms of fatty acid-induced inhibition of glucose uptake.
Boden, G., Chen, X., Ruiz, J., White, J. V., & Rossetti, L. (1994) · Journal of Clinical Investigation · doi:10.1172/jci117252 · Cited by 3,720
Fat Mobilization in Adipose Tissue Is Promoted by Adipose Triglyceride Lipase
Zimmermann, R., Bogner‐Strauß, J. G., Haemmerle, G., Schoiswohl, G., Birner‐Gruenberger, R., Riederer, M., Lass, A., Neuberger, G., Eisenhaber, F., Hermetter, A., et al. (2004) · Science · doi:10.1126/science.1100747 · Cited by 2,121
Molecular Determinants of Glucocorticoid Receptor Function and Tissue Sensitivity to Glucocorticoids
Bamberger, C. M., Schulte, H. M., & Chrousos, G. P. (1996) · Endocrine Reviews · doi:10.1210/edrv-17-3-245 · Cited by 912
Pharmacokinetics and pharmacodynamics of systemically administered glucocorticoids.
Czock, D., Keller, F., Rasche, F. M., & H??ussler, U. (2005) · Clinical Pharmacokinetics · doi:10.2165/00003088-200544010-00003 · Cited by 886
Schweiger, M., Schreiber, R., Haemmerle, G., Lass, A., Fledelius, C., Jacobsen, P., Tornqvist, H. E., Zechner, R., & Zimmermann, R. (2006) · Journal of Biological Chemistry · doi:10.1074/jbc.m608048200 · Cited by 739
Villena, J. A., Roy, S., Sarkadi‐Nagy, E., Kim, K., & Sul, H. S. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m403855200 · Cited by 635
Sztalryd, C., Xu, G., Dorward, H. M., Tansey, J. T., Contreras, J. A., Kimmel, A. R., & Londos, C. (2003) · The Journal of Cell Biology · doi:10.1083/jcb.200210169 · Cited by 535
Self-citationAnthonsen, M. W., Rönnstrand, L., Wernstedt, C., Degerman, E., & Holm, C. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.1.215 · Cited by 468
Macfarlane, D. P., Forbes, S., & Walker, B. R. (2008) · Journal of Endocrinology · doi:10.1677/joe-08-0054 · Cited by 385
Rapid Actions of Steroid Receptors in Cellular Signaling Pathways
Cato, A. C. B., Nestl, A., & Mink, S. (2002) · Science s STKE · doi:10.1126/stke.2002.138.re9 · Cited by 372
Hormone-sensitive lipase of rat adipose tissue. Purification and some properties.
Fredrikson, G., Strålfors, P., Nilsson, N. Ö., & Belfrage, P. (1981) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)69164-7 · Cited by 341
Insulin resistance in type 2 diabetes: role of fatty acids
Arner, P. (2002) · Diabetes/Metabolism Research and Reviews · doi:10.1002/dmrr.254 · Cited by 324
Honnor, R. C., Dhillon, G. S., & Londos, C. (1985) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)95711-x · Cited by 298
Exton, J. H., Friedmann, N. K., Wong, E., Brineaux, J. P., Corbin, J. D., & Park, C. R. (1972) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)45180-6 · Cited by 283
Fain, J. N., KOVACEV, V. P., & Scow, R. O. (1965) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)97175-9 · Cited by 273
Xu, G., Sztalryd, C., Lu, X., Tansey, J. T., Gan, J.-W., Dorward, H. M., Kimmel, A. R., & Londos, C. (2005) · Journal of Biological Chemistry · doi:10.1074/jbc.m506569200 · Cited by 264
Self-citationRole of PAT proteins in lipid metabolism
Londos, C., Sztalryd, C., Tansey, J. T., & Kimmel, A. R. (2005) · Biochimie · doi:10.1016/j.biochi.2004.12.010 · Cited by 261
Hormonal regulation of hormone-sensitive lipase activity and mRNA levels in isolated rat adipocytes.
Slavin, B. G., Ong, J. M., & Kern, P. A. (1994) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)41151-4 · Cited by 261
On the Control of Lipolysis in Adipocytes
Londos, C., Brasaemle, D., Schultz, C. J., Adler‐Wailes, D. C., LEVIN, D. M., Kimmel, A. R., & Rondinone, C. M. (1999) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.1999.tb07794.x · Cited by 251
Mapping of Early Signaling Events in Tumor Necrosis Factor-α-mediated Lipolysis in Human Fat Cells
Rydén, M., Dicker, A., van Harmelen, V., Hauner, H., Brunnberg, M., Perbeck, L. G., Lönnqvist, F., & Arner, P. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m109498200 · Cited by 249
Translocation of Hormone-sensitive Lipase and Perilipin upon Lipolytic Stimulation of Rat Adipocytes
Clifford, G. M., Londos, C., Kraemer, F. B., Vernon, R. G., & Yeaman, S. J. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.275.7.5011 · Cited by 247
An Effect of Insulin on Cyclic Adenosine 3′:5′-Monophosphate Phosphodiesterase Activity in Fat Cells
Manganiello, V. C., & Vaughan, M. M. (1973) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)43374-7 · Cited by 242
Effects of cortisol on lipolysis and regional interstitial glycerol levels in humans
Djurhuus, C. B., Gravholt, C. H., Nielsen, S., Mengel, A., Christiansen, J. S., Schmitz, O. E., & Møller, N. (2002) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00544.2001 · Cited by 219
Mifepristone (RU 486) -- A Modulator of Progestin and Glucocorticoid Action
Wood, A. J. J., Spitz, I. M., & Bardin, C. W. (1993) · New England Journal of Medicine · doi:10.1056/nejm199308053290607 · Cited by 219
Effects of Glucocorticoids on Metabolism of Adipose Tissue in Vitro
Fain, J. N., Scow, R. O., & Chernick, S. S. (1963) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)83960-1 · Cited by 208
Brasaemle, D., Levin, D. M., Adler‐Wailes, D. C., & Londos, C. (2000) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/s1388-1981(99)00179-1 · Cited by 205
Stimulation of Lipolysis in Humans by Physiological Hypercortisolemia
Divertie, G. D., Jensen, M. D., & Miles, J. M. (1991) · Diabetes · doi:10.2337/diab.40.10.1228 · Cited by 201
Regulation and function of triacylglycerol lipases in cellular metabolism
Watt, M. J., & Steinberg, G. R. (2008) · Biochemical Journal · doi:10.1042/bj20080305 · Cited by 165
Glucocorticoid hormone binding to human adipose tissue
Lundholm, K., Rebuffé-Scrive, M., & BJC‐RNTORP, P. (1985) · European Journal of Clinical Investigation · doi:10.1111/j.1365-2362.1985.tb00182.x · Cited by 159
Zu, L., He, J., Jiang, H., Xu, C. B., Pu, S., & Xu, G. (2009) · Journal of Biological Chemistry · doi:10.1074/jbc.m807852200 · Cited by 155
Self-citationPost-translational Regulation of Perilipin Expression
Brasaemle, D., Barber, T., Kimmel, A. R., & Londos, C. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.14.9378 · Cited by 138
Additive effects of cortisol and growth hormone on regional and systemic lipolysis in humans
Djurhuus, C. B., Gravholt, C. H., Nielsen, S., Pedersen, S. B., Møller, N., & Schmitz, O. (2004) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00199.2003 · Cited by 131
Low-dose dexamethasone in the rat: a model to study insulin resistance
Severino, C., Brizzi, P., Solinas, A., Secchi, G., Maioli, M., & Tonolo, G. (2002) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00185.2001 · Cited by 116
Degradation of perilipin is mediated through ubiquitination-proteasome pathway
Xu, G., Sztalryd, C., & Londos, C. (2006) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/j.bbalip.2005.12.005 · Cited by 109
Self-citationMolecular determinants of glucocorticoid receptor function and tissue sensitivity to glucocorticoids
Bamberger, C. M. (1996) · Endocrine Reviews · doi:10.1210/er.17.3.245 · Cited by 89
Heat shock protein 70 is translocated to lipid droplets in rat adipocytes upon heat stimulation
Jiang, H., He, J., Pu, S., Tang, C., & Xu, G. (2006) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/j.bbalip.2006.10.004 · Cited by 68
Self-citationPermissive Effects of Hormones on Lipolysis1
GOODMAN, H. M. (1970) · Endocrinology · doi:10.1210/endo-86-5-1064 · Cited by 68
Lacasa, D., Agli, B., & Giudicelli, Y. (1988) · Biochemical and Biophysical Research Communications · doi:10.1016/s0006-291x(88)81121-5 · Cited by 63
Ren, T., He, J., Jiang, H., Zu, L., Pu, S., Guo, X., & Xu, G. (2006) · Journal of Molecular Endocrinology · doi:10.1677/jme.1.02061 · Cited by 63
Self-citationMechanisms of metformin inhibiting lipolytic response to isoproterenol in primary rat adipocytes
Zhang, T., He, J., Xu, C. B., Zu, L., Jiang, H., Pu, S., Guo, X., & Xu, G. (2008) · Journal of Molecular Endocrinology · doi:10.1677/jme-08-0130 · Cited by 57
Self-citationFricke, K., Heitland, A., & Maronde, E. (2004) · Endocrinology · doi:10.1210/en.2004-0803 · Cited by 49
Salicylate Blocks Lipolytic Actions of Tumor Necrosis Factor-α in Primary Rat Adipocytes
Zu, L., Jiang, H., He, J., Xu, C. B., Pu, S., Liu, M., & Xu, G. (2007) · Molecular Pharmacology · doi:10.1124/mol.107.039479 · Cited by 40
Self-citationStimulation of lipolysis in humans by physiological hypercortisolemia
Divertie, G. D., Jensen, M., & Miles, J. J. (1991) · Diabetes · doi:10.2337/diabetes.40.10.1228 · Cited by 37
He, J., Jiang, H., Tansey, J. T., Tang, C., Pu, S., & Xu, G. (2006) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/j.bbalip.2006.02.001 · Cited by 36
Self-citationElks, M. L., Manganiello, V. C., & Vaughan, M. M. (1984) · Endocrinology · doi:10.1210/endo-115-4-1350 · Cited by 21
Novelli, M., Pocai, A., Chiellini, C., Maffei, M., & Masiello, P. (2007) · Diabetes/Metabolism Research and Reviews · doi:10.1002/dmrr.785 · Cited by 19
Osegawa, M., Makino, H., Kanatsuka, A., SUZUKI, T. A., & Yoshida, S. (1984) · Metabolism · doi:10.1016/0026-0495(84)90218-x · Cited by 9
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Metadata
- Authors
- Xu, C., He, J., Jiang, H., Zu, L., Zhai, W., Pu, S., & Xu, G.
- Affiliations
- Peking University (CN)
- Zhengzhou University (CN)
- Corresponding author
- Xu, Guoheng
- Venue
- Molecular Endocrinology
- Publisher
- The Endocrine Society
- Published
- 2009-08
- Volume
- 23
- Issue
- 8
- Pages
- 1161-1170
- ISSN
- 0888-8809 (ISSN-L)
- 1944-9917
- PMC
- PMC5419195
- OpenAlex
- W2115274616
- Cited by
- 291
- References
- 46
- Citations by year
- 2026: 12 · 2025: 13 · 2024: 22 · 2023: 22 · 2022: 14 · 2021: 20 · 2020: 21 · 2019: 20 · 2018: 16 · 2017: 26 · 2016: 22 · 2015: 16 · 2014: 16 · 2013: 17 · 2012: 17
- FWCI
- 6.00
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Molecular Endocrinology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- University of Pittsburgh
- National Natural Science Foundation of China (30770803, 30890042, 30670779)
- Peking University
- National Key Research and Development Program of China (2006CB 503910, 2009CB941603)
- Peking University Health Science Center
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Health Sciences › Medicine › Physiology (medical) › Adipose Tissue and Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Peroxisome Proliferator-Activated Receptors
- Keywords
- glucocorticoids, dexamethasone, adipocyte lipolysis, free fatty acids, cyclic AMP, protein kinase A, PDE3B, perilipin, hormone-sensitive lipase, adipose triglyceride lipase, hypercortisolism, RU486, H89, lipid droplets
- MeSH terms
- Adipose Tissue, Animals, Dexamethasone, Dose-Response Relationship, Drug, Enzyme Inhibitors, Glucocorticoids, Isoquinolines, Lipolysis, Male, Models, Biological, Serine, Sulfonamides, Rats, Sprague-Dawley, Adipocytes, Rats, Cyclic Nucleotide Phosphodiesterases, Type 3
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