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Cited by 261

The DOI matches its Crossref record; 11 of the 11 references it cites were found in a registry or an index.

Identification of RIFL, a novel adipocyte-enriched insulin target gene with a role in lipid metabolism

Ren, Gang, Kim, Ji Young, Smas, Cynthia M.·2012·American Journal of Physiology-Endocrinology and Metabolism
DOI 10.1152/ajpendo.00084.2012
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Abstract

To identify new genes that are important in fat metabolism, we utilized the Lexicon-Genentech knockout database of genes encoding transmembrane and secreted factors and whole murine genome transcriptional profiling data that we generated for 3T3-L1 in vitro adipogenesis. Cross-referencing null models evidencing metabolic phenotypes with genes induced in adipogenesis led to identification of a new gene, which we named RIFL (refeeding induced fat and liver). RIFL-null mice have serum triglyceride levels approximately one-third of wild type. RIFL transcript is induced >100-fold during 3T3-L1 adipogenesis and is also increased markedly during adipogenesis of murine and human primary preadipocytes. siRNA-mediated knockdown of RIFL during 3T3-L1 adipogenesis results in an ∼35% decrease in adipocyte triglyceride content. Murine RIFL transcript is highly enriched in white and brown adipose tissue and liver. Fractionation of WAT reveals that RIFL transcript is exclusive to adipocytes with a lack of expression in stromal-vascular cells. Nutritional and hormonal studies are consistent with a prolipogenic function for RIFL. There is evidence of an approximately eightfold increase in RIFL transcript level in WAT in ob/ob mice compared with wild-type mice. RIFL transcript level in WAT and liver is increased ∼80- and 12-fold, respectively, following refeeding of fasted mice. Treatment of 3T3-L1 adipocytes with insulin increases RIFL transcript ≤35-fold, whereas agents that stimulate lipolysis downregulate RIFL. Interestingly, the 198-amino acid RIFL protein is predicted to be secreted and shows ∼30% overall conservation with the NH 2-terminal half of angiopoietin-like 3, a liver-secreted protein that impacts lipid metabolism. In summary, our data suggest that RIFL is an important new regulator of lipid metabolism.

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Crossref · 10.1152/ajpendo.00084.2012

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  • ANGPTL8 is expressed primarily in the adipose tissue and liver of mice, and its expression is much higher in the fed state than in the fasted state.

    “All three studies also found that ANGPTL8 is expressed primarily in the adipose tissue and liver of mice and that expression was much higher in the fed state than in the fasted state.”

    From Regulation of lipoprotein metabolism by ANGPTL3, ANGPTL4, and ANGPTL8 (2021) · cites this paper as [139]

  • Mice lacking ANGPTL8 have much lower than normal plasma triglyceride levels.

    “They reported that mice lacking ANGPTL8 had much lower than normal plasma triglyceride levels”

    From Regulation of lipoprotein metabolism by ANGPTL3, ANGPTL4, and ANGPTL8 (2021) · cites this paper as [139]

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Metadata

Authors
Ren, G., Kim, J. Y., & Smas, C. M.
Affiliations
Department of Biochemistry and Cancer Biology and Center for Endocrine and Diabetes Research, University of Toledo College of Medicine, Toledo, Ohio
Venue
American Journal of Physiology-Endocrinology and Metabolism
Publisher
American Physiological Society
Published
2012-08-01
Volume
303
Issue
3
Pages
E334-E351
ISSN
  • 0193-1849
  • 1522-1555
DOI
10.1152/ajpendo.00084.2012
Cited by
261
References
11
Language
en

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