Impaired Fertility and FSH Synthesis in Gonadotrope-Specific Foxl2 Knockout Mice

Tran, Stella; Zhou, Xiang; Lafleur, Christine; Calderon, Michael J.; Ellsworth, Buffy S.; Kimmins, Sarah; Boehm, Ulrich; Treier, Mathias; Boerboom, Derek; Bernard, Daniel J.

doi:10.1210/me.2012-1286

Cited by 74Hypothalamic control of reproductive hormonesReproductive Biology and FertilitySperm and Testicular Function

Impaired Fertility and FSH Synthesis in Gonadotrope-Specific Foxl2 Knockout Mice

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Tran, Stella, Zhou, Xiang, Lafleur, Christine, Calderon, Michael J., Ellsworth, Buffy S., Kimmins, Sarah, Boehm, Ulrich, Treier, Mathias +2 more·2013·Molecular Endocrinology

DOI 10.1210/me.2012-1286PMC PMC3589670

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Abstract

Impairments in pituitary FSH synthesis or action cause infertility. However, causes of FSH dysregulation are poorly described, in part because of our incomplete understanding of mechanisms controlling FSH synthesis. Previously, we discovered a critical role for forkhead protein L2 (FOXL2) in activin-stimulated FSH β-subunit (Fshb) transcription in immortalized cells in vitro. Here, we tested the hypothesis that FOXL2 is required for FSH synthesis in vivo. Using a Cre/lox approach, we selectively ablated Foxl2 in murine anterior pituitary gonadotrope cells. Conditional knockout (cKO) mice developed overtly normally but were subfertile in adulthood. Testis size and spermatogenesis were significantly impaired in cKO males. cKO females exhibited reduced ovarian weight and ovulated fewer oocytes in natural estrous cycles compared with controls. In contrast, ovaries of juvenile cKO females showed normal responses to exogenous gonadotropin stimulation. Both male and female cKO mice were FSH deficient, secondary to diminished pituitary Fshb mRNA production. Basal and activin-stimulated Fshb expression was similarly impaired in Foxl2 depleted primary pituitary cultures. Collectively, these data definitively establish FOXL2 as the first identified gonadotrope-restricted transcription factor required for selective FSH synthesis in vivo.

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Although multiple mouse models have been used to recapitulate idiopathic hypogonadal hypogonadism (IHH), none have focused on loss of function in cells expressing GnRHR and how ERK signaling may be functioning in these tissue compartments.
“Although multiple mouse models have been used to recapitulate idiopathic hypogonadal hypogonadism (IHH), none have focused on loss of function in cells expressing GnRHR and how ERK signaling may be functioning in these tissue compartments3,64,65.”
From Conditional loss of ERK1 and ERK2 results in abnormal placentation and delayed parturition in the mouse (2019) · cites this paper as [64]

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Activin Stabilizes Follicle-Stimulating Hormone-Beta Messenger Ribonucleic Acid Levels
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Regulation of Gonadotropin Subunit Gene Transcription by Gonadotropin-Releasing Hormone: Measurement of Primary Transcript Ribonucleic Acids by Quantitative Reverse Transcription-Polymerase Chain Reaction Assays**This work was supported by NIH Grants HD-11489 and HD-33039 (to J.C.M.), by postdoctoral fellowships T-32-HD-07382 and F-32-HD-08572 (to L.L.B.) and by the Core Laboratories of Specialized Collaborative Center Program for Research in Reproduction Grant U-54-HD-28934.
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Gonadotropin-Releasing Hormone Regulation of Gonadotropin Subunit Gene Expression in Female Rats: Actions on Follicle-Stimulating Hormoneβ Messenger Ribonucleic Acid (mRNA) Involve Differential Expression of Pituitary Activin (β-B) and Follistatin mRNAs1
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Follicle-Stimulating Hormone (FSH) Regulates the Expression of FSH Receptor Messenger Ribonucleic Acid in Cultured Sertoli Cells and in Hypophysectomized Rat Testis1
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Isolated follicle-stimulating hormone (FSH) deficiency without mutation of the FSHβ gene and successful treatment with human menopausal gonadotropin
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Authors: Tran, S., Zhou, X., Lafleur, C., Calderon, M. J., Ellsworth, B. S., Kimmins, S., Boehm, U., Treier, M., Boerboom, D., & Bernard, D. J.
Affiliations: McGill University (CA)
Southern Illinois University Carbondale (US)
Saarland University (DE)
Max Delbrück Center (DE)
Université de Montréal (CA)
Corresponding author: Bernard, Daniel J.
ORCID: Tran, Stella
Zhou, Xiang
Lafleur, Christine
Calderon, Michael J.
Ellsworth, Buffy S.
Kimmins, Sarah
Boehm, Ulrich
Treier, Mathias
Boerboom, Derek
Bernard, Daniel J.
Venue: Molecular Endocrinology
Publisher: The Endocrine Society
Published: 2013-03-01
Volume: 27
Issue: 3
Pages: 407-421
ISSN: 0888-8809 (ISSN-L)
1944-9917
DOI: 10.1210/me.2012-1286
PMC: PMC3589670
OpenAlex: W2079682188
Cited by: 74
References: 93
Citations by year: 2026: 3 · 2025: 5 · 2024: 3 · 2023: 5 · 2022: 1 · 2021: 4 · 2020: 6 · 2019: 8 · 2018: 8 · 2017: 6 · 2016: 5 · 2015: 5 · 2014: 7 · 2013: 8
FWCI: 10.06
Type: journal article
Language: en
Open access: green
europepmc.org
Locations: Molecular Endocrinology (publishedVersion)
Europe PMC (PubMed Central) (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
MDC Repository (Max-Delbrueck-Center for Molecular Medicine) (submittedVersion)
Funders: Canadian Institutes of Health Research (MOP89991)
Eunice Kennedy Shriver National Institute of Child Health and Human Development (U54HD028934, R15HD063469, U54-HD-28934)
Topics: Health Sciences › Medicine › Reproductive Medicine › Hypothalamic control of reproductive hormones
Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Reproductive Biology and Fertility
Health Sciences › Medicine › Reproductive Medicine › Sperm and Testicular Function
Keywords: FOXL2, FSH beta subunit, gonadotrophs, anterior pituitary, infertility, spermatogenesis, activin signaling, transcription factors, conditional knockout mice, gonadotropin deficiency
MeSH terms: Forkhead Box Protein L2, Animals, Female, Fertility, Gene Expression Regulation, Gonadotropins, Ovarian Follicle, Horses, Humans, Male, Organ Specificity, Organ Size, Ovulation, Recombination, Genetic, RNA, Messenger, Sertoli Cells, Spermatogenesis, Testis, Gene Deletion, Mice, Knockout, Activins, Follicle Stimulating Hormone, beta Subunit, Mice, Forkhead Transcription Factors, Gonadotrophs, Genetic Loci

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Impaired Fertility and FSH Synthesis in Gonadotrope-Specific Foxl2 Knockout Mice

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