Insulin regulates the expression of the insulin-like growth factor binding protein 2 mRNA in rat hepatocytes.
Abstract
The goal of this study was to find out whether GH or insulin regulate the mRNA expression of the fetal binding protein of insulin-like growth factor (IGFBP-2). Primary hepatocytes from adult rats were used as a test system. IGFBP-2 mRNA was abundant in cells cultured in the absence of hormones and markedly reduced in cultures containing insulin. Addition of GH had no effect on IGFBP-2 mRNA levels although the cells are responsive to GH as demonstrated by a GH mediated elevation of IGF l mRNA levels. Half-maximal down-regulation of IGFBP-2 mRNA levels occurred at an insulin concentration of 1 to 2 x 10(-10) M. The finding that insulin is a potent negative regulator of hepatic IGFBP-2 mRNA levels suggests a physiologically important regulatory link between the two hormones insulin and IGF l.
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Hepatic Igfbp-2 expression is tonically inhibited by insulin and dramatically up-regulated by insulin deficiency.
“hepatic Igfbp-2 expression is tonically inhibited by insulin and hence dramatically up-regulated by insulin deficiency (50 -52).”
From Leptin Activates a Novel CNS Mechanism for Insulin-Independent Normalization of Severe Diabetic Hyperglycemia (2011) · cites this paper as [50]
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Leptin and the Central Nervous System Control of Glucose Metabolism
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“Since insulin treatment of diabetic rodents lowers both HGP and Igfbp2 gene expression (26, 138, 194), the notion that IGFBP2 plays a major role in leptin-mediated inhibition of HGP seems counterintuitive.”
BackgroundGerman, J. P., Thaler, J. P., Wisse, B. E., Oh-I, S., Sarruf, D. A., Matsen, M. E., Fischer, J. D., Taborsky, G. J., Schwartz, M. W., & Morton, G. J. (2011) · Endocrinology · doi:10.1210/en.2010-0890 · Cited by 169 · see citing works
Allen, N. E., Appleby, P. N., Kaaks, R., Rinaldi, S., Davey, G. K., & Key, T. J. (2003) · Cancer Causes & Control · doi:10.1023/a:1022518321634 · Cited by 104 · see citing works
- Authors
- Böni-Schnetzler, M., Schmid, C., Mary, J.-L., Zimmerli, B., Meier, P. J., Zapf, J., Schwander, J., & Froesch, E. R.
- Affiliations
- University Hospital Zurich (CH)
- ORCID
- Schmid, C.
- Venue
- Molecular Endocrinology
- Publisher
- The Endocrine Society
- Published
- 1990-09
- Volume
- 4
- Issue
- 9
- Pages
- 1320-1326
- ISSN
- 0888-8809 (ISSN-L)
- 1944-9917
- OpenAlex
- W1993694071
- Cited by
- 80
- References
- 37
- Citations by year
- 2026: 1 · 2021: 1 · 2019: 1 · 2018: 1 · 2017: 2 · 2016: 2 · 2015: 3 · 2014: 3 · 2012: 4
- FWCI
- 5.18
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Molecular Endocrinology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung
- Topics
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Growth Hormone and Insulin-like Growth Factors
- Health Sciences › Medicine › Hepatology › Liver physiology and pathology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic Syndromes and Imprinting
- Keywords
- insulin, IGFBP-2, hepatocytes, growth hormone, insulin-like growth factor I, hepatic gene regulation, hormone regulation of gene expression
- MeSH terms
- Animals, Carrier Proteins, Cells, Cultured, Dose-Response Relationship, Drug, Gene Expression Regulation, Insulin, Liver, Male, Nucleic Acid Hybridization, Rats, Inbred Strains, RNA, Messenger, Growth Hormone, Insulin-Like Growth Factor Binding Proteins, Rats
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