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Cited by 80Ion channel regulation and functionIon Channels and ReceptorsPancreatic function and diabetes

Differential expression of ionic channels in rat anterior pituitary cells

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Van Goor, Fredrick, Zivadinovic, Dragoslava, Stojilkovic, Stanko S.·2001·Molecular Endocrinology
DOI 10.1210/mend.15.7.0668
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Abstract

Secretory anterior pituitary cells are of the same origin, but exhibit cell type-specific patterns of spontaneous intracellular Ca2+ signaling and basal hormone secretion. To understand the underlying ionic mechanisms mediating these differences, we compared the ionic channels expressed in somatotrophs, lactotrophs, and gonadotrophs from randomly cycling female rats under identical cell culture and recording conditions. Our results indicate that a similar group of ionic channels are expressed in each cell type, including transient and sustained voltage-gated Ca2+ channels, tetrodotoxin-sensitive Na+ channels, transient and delayed rectifying K+ channels, and multiple Ca2+ -sensitive K+ channel subtypes. However, there were marked differences in the expression levels of some of the ionic channels. Specifically, lactotrophs and somatotrophs exhibited low expression levels of tetrodotoxin-sensitive Na+ channels and high expression levels of the large-conductance, Ca2+ -activated K+ channel compared with those observed in gonadotrophs. In addition, functional expression of the transient K+ channel was much higher in lactotrophs and gonadotrophs than in somatotrophs. Finally, the expression of the transient voltage-gated Ca2+ channels was higher in somatotrophs than in lactotrophs and gonadotrophs. These results indicate that there are cell type-specific patterns of ionic channel expression, which may be of physiological significance for the control of Ca2+ homeostasis and secretion in unstimulated and receptor-stimulated anterior pituitary cells.

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  • Bursts provide more time for Ca2+ to enter cells, so lactotrophs and somatotrophs have a high basal rate of hormone release.

    “Bursts provide more time for Ca2+ to enter cells, so lactotrophs and somatotrophs have a high basal rate of hormone release [70].”

    From Mathematical Modelling of Endocrine Systems (2019) · cites this paper as [70]

    The Introduction (page unavailable) says lactotrophs and somatotrophs have high basal secretion alongside high-amplitude, extracellular-Ca²⁺-dependent signals. The Discussion (page unavailable) reports longer action-potential waveforms and prolonged Ca²⁺ entry in these cells. Our copy does not specifically identify bursts as the cause of their high basal hormone release. (LLM, conf=0.83)

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  • In the absence of hypothalamic signals, pituitary gonadotrophs fire sharp spikes at a slow rate, releasing very little hormone.

    “In the absence of hypothalamic signals, pituitary gonadotrophs fire sharp spikes at a slow rate, releasing very little hormone.”

    From Mathematical Modelling of Endocrine Systems (2019) · cites this paper as [70]

    The Introduction (page unavailable) says unstimulated gonadotrophs have low basal gonadotropin secretion, and the Discussion (page unavailable) describes their action potentials as shorter than those of somatotrophs and lactotrophs. Our copy does not establish that gonadotrophs fire at a slow rate or that their low basal secretion results from that firing pattern. (LLM, conf=0.86)

    Experimental — accuracy not yet measured

  • Lactotrophs and somatotrophs fire in bursts, which are longer electrical events than spikes.

    “By contrast, lactotrophs and somatotrophs fire in bursts, which are longer electrical events than spikes.”

    From Mathematical Modelling of Endocrine Systems (2019) · cites this paper as [70]

    The Discussion (page unavailable) reports that action-potential waveforms last longer in rat somatotrophs and lactotrophs (100–500 ms) than in gonadotrophs (10–100 ms). It does not identify those events as bursts or compare burst duration with spike duration, so those parts of the claim are unsupported. (LLM, conf=0.83)

    Experimental — accuracy not yet measured

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Authors
Van Goor, F., Zivadinovic, D., & Stojilkovic, S. S.
Affiliations
Endocrinology and Reproduction Research Branch National Institute of Child Health and Human Development National Institutes of Health Bethesda, Maryland 20892-4510
Venue
Molecular Endocrinology
Publisher
The Endocrine Society
Published
2001-07-01
Volume
15
Issue
7
Pages
1222-1236
ISSN
  • 0888-8809 (ISSN-L)
  • 1944-9917
DOI
10.1210/mend.15.7.0668
OpenAlex
W2160926336
Cited by
80
References
54
Citations by year
2025: 1 · 2024: 1 · 2023: 1 · 2022: 4 · 2021: 3 · 2019: 4 · 2017: 6 · 2016: 4 · 2015: 1 · 2014: 3 · 2013: 2 · 2012: 4
FWCI
1.16
Type
journal article
Language
en
Open access
Locations
  • Molecular Endocrinology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
  • Life Sciences › Neuroscience › Sensory Systems › Ion Channels and Receptors
  • Health Sciences › Medicine › Surgery › Pancreatic function and diabetes
Keywords
ion channels, anterior pituitary, somatotrophs, lactotrophs, gonadotrophs, voltage-gated calcium channels, tetrodotoxin-sensitive sodium channels, potassium channels, BK channels, cell type-specific gene expression, calcium homeostasis, hormone secretion, intracellular calcium signaling
MeSH terms
Animals, Calcium, Cells, Cultured, Electrophysiology, Female, Gonadotropins, Pituitary, Humans, Ion Channels, Pituitary Gland, Anterior, Prolactin, Growth Hormone, Tetrodotoxin, Calcium Channels, Potassium Channels, Sodium Channels, Ion Channel Gating, Gene Expression, Rats, Sprague-Dawley, Rats

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