Suppression of adjuvant arthritis by hesperidin in rats and its mechanisms
Abstract
The citrus flavonoid hesperidin has been reported to possess a wide range of pharmacological properties. We have investigated the preventive and therapeutic effects of hesperidin on the development of adjuvant arthritis (AA), a rat model of rheumatoid arthritis (RA). Freund's complete adjuvant was used to induce AA in rats. Secondary paw swelling, polyarthritis index and histopathological assessment of ankle joints were used to evaluate the effects of hesperidin on AA rats. Concanavalin-A-induced T-lymphocyte proliferation and interleukin (IL)-2 production by splenocytes were measured using the MTT assay. Levels of IL-1, IL-6 and tumour necrosis factor (TNF)-alpha secreted by peritoneal macrophages (PM) were measured by RIA. Intragastric administration of hesperidin significantly attenuated secondary paw swelling and reduced the polyarthritis index of AA rats in a dose-dependent manner. In addition, hesperidin clearly ameliorated the pathological changes in AA rats. Hesperidin also restored the suppression of T-lymphocyte proliferation and IL-2 production, and downregulated production of IL-1, IL-6 and TNF-alpha by PM in AA rats. Our results suggest that hesperidin improves AA by downregulating the function of over-active macrophages and by up-regulating the activities of dysfunctional T lymphocytes. Hesperidin may therefore have therapeutic value for the clinical treatment of RA. Further research is required to clarify the detailed mechanisms of the protective effects of hesperidin on AA.
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Administration of the flavonoid hesperidin can reduce secretion of cytokines interleukin 1 and TNFα and restricts inflammation in a murine rheumatic arthritis model.
“As Li et al. [18] proved, the administration of the flavonoid hesperidin can reduce the secretion of cytokines like interleukin 1 (Il-1) and TNFα and restricts the inflammation in a murine rheumatic arthritis model [18].”
From Mediterranean Diet as an Antioxidant: The Impact on Metabolic Health and Overall Wellbeing (2021) · cites this paper as [18]
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Mediterranean Diet as an Antioxidant: The Impact on Metabolic Health and Overall Wellbeing
Gantenbein, K. V., & Kanaka-Gantenbein, C. (2021) · Nutrients · doi:10.3390/nu13061951 · Cited by 288 · see citing works
- Authors
- Li, R., Li, J., Cai, L., Hu, C.-M., & Zhang, L.
- Affiliations
- Anhui Medical University (CN)
- School of Pharmacy, Anhui Medical University , 81 Meishan Road, Hefei,
- Corresponding author
- Li, Jun
- Venue
- Journal of Pharmacy and Pharmacology
- Publisher
- Oxford University Press (OUP)
- Published
- 2008-02-01
- Volume
- 60
- Issue
- 2
- Pages
- 221-228
- ISSN
- 0022-3573 (ISSN-L)
- 2042-7158
- OpenAlex
- W1994972665
- Cited by
- 84
- References
- 37
- Citations by year
- 2025: 5 · 2024: 1 · 2023: 6 · 2022: 3 · 2021: 10 · 2020: 7 · 2019: 7 · 2018: 7 · 2017: 3 · 2016: 4 · 2015: 5 · 2014: 7 · 2013: 1 · 2012: 8
- FWCI
- 2.21
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
- Locations
- Journal of Pharmacy and Pharmacology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Rheumatology › Rheumatoid Arthritis Research and Therapies
- Health Sciences › Medicine › Complementary and alternative medicine › Natural Compounds in Disease Treatment
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Bioactive Compounds in Plants
- Keywords
- hesperidin, adjuvant-induced arthritis, rheumatoid arthritis, flavonoids, T cell proliferation, interleukin-2, interleukin-1, interleukin-6, TNF-α, peritoneal macrophages, anti-inflammatory activity
- MeSH terms
- Animals, Anti-Inflammatory Agents, Arthritis, Experimental, Arthritis, Rheumatoid, Disease Models, Animal, Dose-Response Relationship, Drug, Freund's Adjuvant, Gene Expression Regulation, Hesperidin, Interleukins, Macrophages, Male, T-Lymphocytes, Tumor Necrosis Factor-alpha, Rats, Sprague-Dawley, Rats
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