The DOI matches its Crossref record; 27 of the 27 references it cites were found in a registry or an index.
Elevated plasma endothelial microparticles in multiple sclerosis
Abstract
OBJECTIVE: To assess endothelial dysfunction in patients with MS and to investigate whether plasma from patients with MS induces endothelial cell dysfunction in vitro. BACKGROUND: Endothelial cell dysfunction may contribute to the pathogenesis of MS. Elevations of soluble adhesion molecules intracellular adhesion molecule, vascular cell adhesion molecule, and platelet-endothelial cell adhesion molecule-1 (CD31) have been reported as markers of blood-brain barrier (BBB) damage in MS, but direct assay of endothelium has been difficult. Endothelial cells release microparticles < approximately 1.5 microm (EMP) during activation or apoptosis. The authors developed a flow cytometric assay of EMP and studied EMP as markers of endothelial damage in MS. METHODS: Platelet-poor plasma (PPP) from 50 patients with MS (30 in exacerbation and 20 in remission) and 48 controls were labeled with fluorescein isothiocyanate (FITC)-conjugated anti-CD31 and anti-CD51 (vitronectin receptor) antibodies, and two classes of EMP (CD31+ and CD51+) were assayed by flow cytometry. For in vitro studies, patients' plasma was added to the microvascular endothelial cell (MVEC) culture and release of CD31+ and CD51+ EMP were measured in the supernatant. RESULTS: Plasma from patients in exacerbation had 2.85-fold elevation of CD31+ EMP as compared with healthy controls, returning to near control value during remission. The CD31+ EMP concentration showed a positive association with gadolinium enhancement in patients with MS. In contrast, CD51+ EMP remained elevated in both exacerbation and remission. This suggests that CD31+ EMP is a marker of acute injury, whereas CD51+ EMP reflects chronic injury of endothelium. MS plasma induced release of both CD31+ and CD51+ EMP from MVEC culture in vitro. CONCLUSION: Endothelial dysfunction is evident during exacerbation of MS, evidenced by shedding of EMP expressing PECAM-1 (CD31). The in vitro data indicate contribution of one or more plasma factors in endothelial dysfunction of MS.
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Cites
New diagnostic criteria for multiple sclerosis: Guidelines for research protocols
Poser, C. M., Paty, D. W., Scheinberg, L., McDonald, W. I., Davis, F. A., Ebers, G. C., Johnson, K. P., Sibley, W. A., Silberberg, D. H., & Tourtellotte, W. W. (1983) · Annals of Neurology · doi:10.1002/ana.410130302 · Cited by 7,384
Traffic signals for lymphocyte recirculation and leukocyte emigration: The multistep paradigm
Springer, T. A. (1994) · Cell · doi:10.1016/0092-8674(94)90337-9 · Cited by 6,929
PECAM-1 is required for transendothelial migration of leukocytes.
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The adhesion molecule and cytokine profile of multiple sclerosis lesions
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Involvement of Platelet-Endothelial Cell Adhesion Molecule-1 in Neutrophil Recruitment in Vivo
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György, B., Szabó, T. G., Pásztói, M., Pál, Z., Misják, P., Aradi, B., László, V., Pállinger, É., Pap, E., Kittel, Á., et al. (2011) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-011-0689-3 · Cited by 2,074
Jimenez, J. J., Jy, W., Mauro, L. M., Soderland, C., Horstman, L. L., & Ahn, Y. S. (2003) · Thrombosis Research · doi:10.1016/s0049-3848(03)00064-1 · Cited by 517
Self-citationEffects of Severe Hypertension on Endothelial and Platelet Microparticles
Preston, R. A., Jy, W., Jimenez, J. J., Mauro, L. M., Horstman, L. L., Valle, M., Aime, G., & Ahn, Y. S. (2003) · Hypertension · doi:10.1161/01.hyp.0000049760.15764.2d · Cited by 499
Self-citationHigh levels of circulating endothelial microparticles in patients with acute coronary syndromes
Bernal-Mizrachi, L., Jy, W., Jimenez, J. J., Pastor, J., Mauro, L. M., Horstman, L. L., de Marchena, E., & Ahn, Y. S. (2003) · American Heart Journal · doi:10.1016/s0002-8703(03)00103-0 · Cited by 441
Self-citationEndothelial microparticles in diseases
Chironi, G. N., Boulanger, C. M., Simon, A., Dignat-George, F., Freyssinet, J.-M., & Tedgui, A. (2009) · Cell and Tissue Research · doi:10.1007/s00441-008-0710-9 · Cited by 420
Metadata
- Authors
- Minagar, A., Jy, W., Jimenez, J. J., Sheremata, W. A., Mauro, L. M., Mao, W. W., Horstman, L. L., & Ahn, Y. S.
- Venue
- Neurology
- Published
- 2001-05-22
- Volume
- 56
- Issue
- 10
- Pages
- 1319-1324
- ISSN
- 0028-3878 (ISSN-L)
- 1526-632X
- OpenAlex
- W2015805487
- Cited by
- 289
- References
- 27
- FWCI
- 7.48
- Type
- journal article
- Language
- en
- Open access
- closed
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