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Cited by 608

The DOI matches its Crossref record; 29 of the 29 references it cites were found in a registry or an index.

Selective plane illumination microscopy techniques in developmental biology

Open access
Huisken, Jan, Stainier, Didier Y. R.·2009·Development

Abstract

Selective plane illumination microscopy (SPIM) and other fluorescence microscopy techniques in which a focused sheet of light serves to illuminate the sample have become increasingly popular in developmental studies. Fluorescence light-sheet microscopy bridges the gap in image quality between fluorescence stereomicroscopy and high-resolution imaging of fixed tissue sections. In addition, high depth penetration, low bleaching and high acquisition speeds make light-sheet microscopy ideally suited for extended time-lapse experiments in live embryos. This review compares the benefits and challenges of light-sheet microscopy with established fluorescence microscopy techniques such as confocal microscopy and discusses the different implementations and applications of this easily adaptable technology.

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Crossref · 10.1242/dev.022426

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Metadata

Authors
Huisken, J., & Stainier, D. Y. R.
Venue
Development
Published
2009-06-15
Volume
136
Issue
12
Pages
1963-1975
ISSN
  • 0950-1991 (ISSN-L)
  • 1477-9129
OpenAlex
W2094643072
Cited by
608
References
29
FWCI
16.50
Type
journal article
Language
en
Open access
green

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