The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Neverland is an evolutionally conserved Rieske-domain protein that is essential for ecdysone synthesis and insect growth
Abstract
Steroid hormones mediate a wide variety of developmental and physiological events in multicellular organisms. During larval and pupal stages of insects,the principal steroid hormone is ecdysone, which is synthesized in the prothoracic gland (PG) and plays a central role in the control of development. Although many studies have revealed the biochemical features of ecdysone synthesis in the PG, many aspects of this pathway have remained unclear at the molecular level. We describe the neverland (nvd) gene, which encodes an oxygenase-like protein with a Rieske electron carrier domain, from the silkworm Bombyx mori and the fruitfly Drosophila melanogaster. nvd is expressed specifically in tissues that synthesize ecdysone, such as the PG. We also show that loss of nvd function in the PG causes arrest of both molting and growth during Drosophiladevelopment. Furthermore, the phenotype is rescued by application of 20-hydroxyecdysone or the precursor 7-dehydrocholesterol. Given that the nvd family is evolutionally conserved, these results suggest that Nvd is an essential regulator of cholesterol metabolism or trafficking in steroid synthesis across animal phyla.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Evidence for a clade of nematodes, arthropods and other moulting animals
Aguinaldo, A. M. A., Turbeville, J. M., Linford, L. S., Rivera, M. C., Garey, J. R., Raff, R. A., & Lake, J. A. (1997) · Nature · doi:10.1038/387489a0 · Cited by 1,609
Control and biochemical nature of the ecdysteroidogenic pathway
Gilbert, L. I., Rybczynski, R., & Warren, J. T. (2002) · Annual Review of Entomology · doi:10.1146/annurev.ento.47.091201.145302 · Cited by 532 · cited 8×
“The nurse cells are considered to be the source of ecdysteroids in adult females (Riddiford, 1993; Gilbert et al., 2002) and a number of ecdysteroidogenic genes, such as dare, Start1 and ecdysoneless, are known to be expressed in the nurse cells (Freeman et al.”
BackgroundMethodInfluentialThe dare gene: steroid hormone production, olfactory behavior, and neural degeneration in Drosophila
Freeman, M. R., Dobritsa, A., Gaines, P., Segraves, W. A., & Carlson, J. R. (1999) · Development · doi:10.1242/dev.126.20.4591 · Cited by 119
Cited by
Insect CYP Genes and P450 Enzymes
Feyereisen, R. (2012) · Insect Molecular Biology and Biochemistry · doi:10.1016/b978-0-12-384747-8.10008-x · Cited by 566
Prothoracicotropic Hormone Regulates Developmental Timing and Body Size in Drosophila
McBrayer, Z., Ono, H., Shimell, M., Parvy, J.-P., Beckstead, R. B., Warren, J. T., Thummel, C. S., Dauphin-Villemant, C., Gilbert, L. I., & O'Connor, M. B. (2007) · Developmental Cell · doi:10.1016/j.devcel.2007.11.003 · Cited by 434
Enzymes for ecdysteroid biosynthesis: Their biological functions in insects and beyond
Niwa, R., & Niwa, Y. S. (2014) · Bioscience, Biotechnology, and Biochemistry · doi:10.1080/09168451.2014.942250 · Cited by 249
Self-citationChafino, S., Salvia, R., Cruz, J., Martín, D., & Franch-Marro, X. (2023) · PLOS Genetics · doi:10.1371/journal.pgen.1010897 · Cited by 11
Metadata
- Authors
- Yoshiyama, T., Namiki, T., Mita, K., Kataoka, H., & Niwa, R.
- Venue
- Development
- Publisher
- The Company of Biologists
- Published
- 2006-07-01
- Volume
- 133
- Issue
- 13
- Pages
- 2565-2574
- ISSN
- 0950-1991 (ISSN-L)
- 1477-9129
- OpenAlex
- W2063689436
- Cited by
- 248
- References
- 3
- FWCI
- 2.20
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Neverland is an evolutionally conserved Rieske-domain protein that is essential for ecdysone synthesis and insect growth. https://app.cochat.ai/papers/10.1242/dev.02428
- The paper
- Yoshiyama, T., Namiki, T., Mita, K., Kataoka, H., & Niwa, R. (2006). Neverland is an evolutionally conserved Rieske-domain protein that is essential for ecdysone synthesis and insect growth. Development. https://doi.org/10.1242/dev.02428
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
