The DOI matches its Crossref record; 32 of the 32 references it cites were found in a registry or an index.
Sox9+ ductal cells are multipotent progenitors throughout development but do not produce new endocrine cells in the normal or injured adult pancreas
Open accessAbstract
One major unresolved question in the field of pancreas biology is whether ductal cells have the ability to generate insulin-producing β-cells. Conclusive examination of this question has been limited by the lack of appropriate tools to efficiently and specifically label ductal cells in vivo. We generated Sox9CreERT2 mice, which, during adulthood, allow for labeling of an average of 70% of pancreatic ductal cells, including terminal duct/centroacinar cells. Fate-mapping studies of the Sox9+ domain revealed endocrine and acinar cell neogenesis from Sox9+ cells throughout embryogenesis. Very small numbers of non-β endocrine cells continue to arise from Sox9+ cells in early postnatal life, but no endocrine or acinar cell neogenesis from Sox9+ cells occurs during adulthood. In the adult pancreas, pancreatic injury by partial duct ligation (PDL) has been suggested to induce β-cell regeneration from a transient Ngn3+ endocrine progenitor cell population. Here, we identify ductal cells as a cell of origin for PDL-induced Ngn3+ cells, but fail to observe β-cell neogenesis from duct-derived cells. Therefore, although PDL leads to activation of Ngn3 expression in ducts, PDL does not induce appropriate cues to allow for completion of the entire β-cell neogenesis program. In conclusion, although endocrine cells arise from the Sox9+ ductal domain throughout embryogenesis and the early postnatal period, Sox9+ ductal cells of the adult pancreas no longer give rise to endocrine cells under both normal conditions and in response to PDL.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
A new mathematical model for relative quantification in real-time RT-PCR
Pfaffl, M. W. (2001) · Nucleic Acids Research · doi:10.1093/nar/29.9.e45 · Cited by 34,610
Generalized lacZ expression with the ROSA26 Cre reporter strain
Soriano, P. (1999) · Nature Genetics · doi:10.1038/5007 · Cited by 4,859
Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locus
Srinivas, S., Watanabe, T., Lin, C.-S., William, C. M., Tanabe, Y., Jessell, T. M., & Costantini, F. (2001) · BMC Developmental Biology · doi:10.1186/1471-213x-1-4 · Cited by 3,121
Adult pancreatic β-cells are formed by self-duplication rather than stem-cell differentiation
Dor, Y., Brown, J., Martinez, O. I., & Melton, D. A. (2004) · Nature · doi:10.1038/nature02520 · Cited by 2,219
Gu, G., Dubauskaite, J., & Melton, D. A. (2002) · Development · doi:10.1242/dev.129.10.2447 · Cited by 1,518
Xu, G., Stoffers, D. A., Habener, J. F., & Bonner-Weir, S. (1999) · Diabetes · doi:10.2337/diabetes.48.12.2270 · Cited by 1,209
The role of the transcriptional regulator Ptf1a in converting intestinal to pancreatic progenitors
Kawaguchi, Y., Cooper, B., Gannon, M., Ray, M., MacDonald, R. J., & Wright, C. V. E. (2002) · Nature Genetics · doi:10.1038/ng959 · Cited by 1,007 · cited 1×
“…cell types, which encompass five different endocrine lineages as well as the exocrine acinar and ductal cells, are derived from a pool of early pancreatic progenitor cells that express the transcription factors Pdx1, Ptf1a and Sox9 (Gu et al., 2002; Kawaguchi et al., 2002; Akiyama et al., 2005).”
Backgroundβ Cells Can Be Generated from Endogenous Progenitors in Injured Adult Mouse Pancreas
Xu, X., D'Hoker, J., Stangé, G., Bonné, S., De Leu, N., Xiao, X., Van De Casteele, M., Mellitzer, G., Ling, Z., Pipeleers, D., et al. (2008) · Cell · doi:10.1016/j.cell.2007.12.015 · Cited by 988
Osteo-chondroprogenitor cells are derived from Sox9 expressing precursors
Akiyama‡, H., Kim‡, J.-E., Nakashima, K., Balmes, G., Iwai, N., Deng, J. M., Zhang, Z., Martin, J. F., Behringer, R. R., Nakamura, T., et al. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0504750102 · Cited by 605
SOX9 is required for maintenance of the pancreatic progenitor cell pool
Seymour, P. A., Freude, K. K., Tran, M. N., Mayes, E. E., Jensen, J., Kist, R., Scherer, G., & Sander, M. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0609217104 · Cited by 567
Self-citationA Multipotent Progenitor Domain Guides Pancreatic Organogenesis
Zhou, Q., Law, A. C., Rajagopal, J., Anderson, W. J., Gray, P. A., & Melton, D. A. (2007) · Developmental Cell · doi:10.1016/j.devcel.2007.06.001 · Cited by 537
Solar, M., Cardalda, C., Houbracken, I., Martín, M., Maestro, M. A., De Medts, N., Xu, X., Grau, V., Heimberg, H., Bouwens, L., et al. (2009) · Developmental Cell · doi:10.1016/j.devcel.2009.11.003 · Cited by 487
Neural crest development is regulated by the transcription factor Sox9
Cheung, M., & Briscoe, J. (2003) · Development · doi:10.1242/dev.00808 · Cited by 482
Inada, A., Nienaber, C., Katsuta, H., Fujitani, Y., Levine, J., Morita, R., Sharma, A., & Bonner-Weir, S. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0805803105 · Cited by 448
Duct- to islet-cell differentiation and islet growth in the pancreas of duct-ligated adult rats
Wang, R. N., Kl�ppel, G., & Bouwens, L. (1995) · Diabetologia · doi:10.1007/bf00400600 · Cited by 438
Vidal, V. P. I., Chaboissier, M.-C., Lützkendorf, S., Cotsarelis, G., Mill, P., Hui, C.-C., Ortonne, N., Ortonne, J.-P., & Schedl, A. (2005) · Current Biology · doi:10.1016/j.cub.2005.06.064 · Cited by 427
Epithelial cell proliferation and islet neogenesis in IFN-g transgenic mice
Gu, D., & Sarvetnick, N. (1993) · Development · doi:10.1242/dev.118.1.33 · Cited by 321
Rovira, M., Scott, S.-G., Liss, A. S., Jensen, J., Thayer, S. P., & Leach, S. D. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0912589107 · Cited by 290
Schaffer, A. E., Freude, K. K., Nelson, S. B., & Sander, M. (2010) · Developmental Cell · doi:10.1016/j.devcel.2010.05.015 · Cited by 279
Self-citationNKX6 transcription factor activity is required for α- andβ-cell development in the pancreas
Henseleit, K. D., Nelson, S. B., Kuhlbrodt, K., Hennings, J. C., Ericson, J., & Sander, M. (2005) · Development · doi:10.1242/dev.01875 · Cited by 200
Self-citationMeans, A. L., Xu, Y., Zhao, A., Ray, K. C., & Gu, G. (2008) · genesis · doi:10.1002/dvg.20397 · Cited by 184
A GRFa2/Prop1/Stem (GPS) Cell Niche in the Pituitary
Garcia-Lavandeira, M., Quereda, V., Flores, I., Saez, C., Diaz-Rodriguez, E., Japon, M. A., Ryan, A. K., Blasco, M. A., Dieguez, C., Malumbres, M., et al. (2009) · PLoS ONE · doi:10.1371/journal.pone.0004815 · Cited by 182
Pancreatic β-Cell Neogenesis by Direct Conversion from Mature α-Cells
Chung, C.-H., Hao, E., Piran, R., Keinan, E., & Levine, F. (2010) · Stem Cells · doi:10.1002/stem.482 · Cited by 181
Self-citationWang, S., Jensen, J. N., Seymour, P. A., Hsu, W., Dor, Y., Sander, M., Magnuson, M. A., Serup, P., & Gu, G. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0904247106 · Cited by 160
Self-citationA dosage-dependent requirement for Sox9 in pancreatic endocrine cell formation
Seymour, P. A., Freude, K., Dubois, C. L., Shih, H.-P., Patel, N. A., & Sander, M. (2008) · Developmental Biology · doi:10.1016/j.ydbio.2008.07.034 · Cited by 143
Self-citationKopinke, D., & Murtaugh, L. C. (2010) · BMC Developmental Biology · doi:10.1186/1471-213x-10-38 · Cited by 117
Beta-cell growth in adolescent and adult rats treated with streptozotocin during the neonatal period
Wang, R. N., Bouwens, L., & Kl�ppel, G. (1996) · Diabetologia · doi:10.1007/bf00403301 · Cited by 92
Blaine, S. A., Ray, K. C., Anunobi, R., GANNON, M. A., Washington, M. K., & Means, A. L. (2010) · Development · doi:10.1242/dev.048421 · Cited by 78
Ductal Origin Hypothesis of Pancreatic Regeneration under Attack
Kushner, J. A., Weir, G. C., & Bonner‐Weir, S. (2010) · Cell Metabolism · doi:10.1016/j.cmet.2009.12.005 · Cited by 65
Fission of pancreatic islets during postnatal growth of the mouse
Seymour, P. A., Bennett, W. R., & Slack, J. M. W. (2004) · Journal of Anatomy · doi:10.1111/j.1469-7580.2004.00265.x · Cited by 36
Self-citationKilic, G., Wang, J., & Sosa‐Pineda, B. (2006) · Developmental Dynamics · doi:10.1002/dvdy.20729 · Cited by 35
Sakaguchi, Y., Inaba, M., Kusafuka, K., Okazaki, K., & Ikehara, S. (2006) · Pancreas · doi:10.1097/01.mpa.0000236734.39241.99 · Cited by 28
Cited by
Slug and Sox9 Cooperatively Determine the Mammary Stem Cell State
Guo, W., Keckesova, Z., Donaher, J. L., Shibue, T., Tischler, V., Reinhardt, F., Itzkovitz, S., Noske, A., Zürrer-Härdi, U., Bell, G., et al. (2012) · Cell · doi:10.1016/j.cell.2012.02.008 · Cited by 957
Kopp, J. L., von Figura, G., Mayes, E., Liu, F.-F., Dubois, C. L., Morris, J. P., Pan, F. C., Akiyama, H., Wright, C. V. E., Jensen, K., et al. (2012) · Cancer Cell · doi:10.1016/j.ccr.2012.10.025 · Cited by 697
Self-citationDe Novo Prediction of Stem Cell Identity using Single-Cell Transcriptome Data
Grün, D., Muraro, M. J., Boisset, J.-C., Wiebrands, K., Lyubimova, A., Dharmadhikari, G., van den Born, M., van Es, J., Jansen, E., Clevers, H., et al. (2016) · Cell Stem Cell · doi:10.1016/j.stem.2016.05.010 · Cited by 586
Acinar cell plasticity and development of pancreatic ductal adenocarcinoma
Storz, P. (2017) · Nature Reviews Gastroenterology & Hepatology · doi:10.1038/nrgastro.2017.12 · Cited by 389
Liver Progenitor Cells Yield Functional Hepatocytes in Response to Chronic Liver Injury in Mice
Español–Suñer, R., Carpentier, R., Van Hul, N., Legry, V., Achouri, Y., Cordi, S., Jacquemin, P., Lemaigre, F., & Leclercq, I. A. (2012) · Gastroenterology · doi:10.1053/j.gastro.2012.08.024 · Cited by 356
Metadata
- Authors
- Kopp, J. L., Dubois, C. L., Schaffer, A. E., Hao, E., Shih, H. P., Seymour, P. A., Ma, J., & Sander, M.
- Venue
- Development
- Publisher
- The Company of Biologists
- Published
- 2011-02-15
- Volume
- 138
- Issue
- 4
- Pages
- 653-665
- ISSN
- 0950-1991 (ISSN-L)
- 1477-9129
- OpenAlex
- W1981362835
- Cited by
- 469
- References
- 32
- FWCI
- 40.38
- Type
- journal article
- Language
- en
- Open access
- bronze
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
