Cited by 67Axon Guidance and Neuronal SignalingDevelopmental Biology and Gene RegulationNeurogenesis and neuroplasticity mechanisms

Engrailed homeoprotein recruits the adenosine A1 receptor to potentiate ephrin A5 function in retinal growth cones

Stettler, Olivier, Joshi, Rajiv L., Wizenmann, Andrea, Reingruber, Jürgen, Holcman, David, Bouillot, Colette, Castagner, François, Prochiantz, Alain +1 more·2012·Development

Abstract

Engrailed 1 and engrailed 2 homeoprotein transcription factors (collectively Engrailed) display graded expression in the chick optic tectum where they participate in retino-tectal patterning. In vitro, extracellular Engrailed guides retinal ganglion cell (RGC) axons and synergises with ephrin A5 to provoke the collapse of temporal growth cones. In vivo disruption of endogenous extracellular Engrailed leads to misrouting of RGC axons. Here we characterise the signalling pathway of extracellular Engrailed. Our results show that Engrailed/ephrin A5 synergy in growth cone collapse involves adenosine A1 receptor activation after Engrailed-dependent ATP synthesis, followed by ATP secretion and hydrolysis to adenosine. This is, to our knowledge, the first evidence for a role of the adenosine A1 receptor in axon guidance. Based on these results, together with higher expression of the adenosine A1 receptor in temporal than nasal growth cones, we propose a computational model that illustrates how the interaction between Engrailed, ephrin A5 and adenosine could increase the precision of the retinal projection map.

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Crossref · 10.1242/dev.063875

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  • In dopaminergic neurons and retinal ganglion cell growth cones, translation of nuclear-encoded mitochondrial mRNAs commonly occurs after homeoprotein capture.

    “Indeed, translation of nuclear-encoded mitochondrial mRNAs commonly follows homeoprotein capture by dopaminergic neurons or retinal ganglion cell growth cones (Alvarez-Fischer et al., 2011; Stettler et al., 2012; Yoon et al., 2012).”

    From Choroid-Plexus-Derived Otx2 Homeoprotein Constrains Adult Cortical Plasticity (2013) · cites this paper as Stettler et al., 2012

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Authors
Stettler, O., Joshi, R. L., Wizenmann, A., Reingruber, J., Holcman, D., Bouillot, C., Castagner, F., Prochiantz, A., & Moya, K. L.
Affiliations
  • Centre National de la Recherche Scientifique (FR)
  • University of Tübingen (DE)
Corresponding author
Prochiantz, Alain
Venue
Development
Published
2012-01-01
Volume
139
Issue
1
Pages
215-224
ISSN
  • 0950-1991 (ISSN-L)
  • 1477-9129
OpenAlex
W2114191977
Cited by
67
References
47
Citations by year
2024: 2 · 2023: 4 · 2022: 3 · 2021: 4 · 2020: 6 · 2019: 7 · 2018: 4 · 2017: 5 · 2016: 4 · 2015: 10 · 2014: 9 · 2013: 4 · 2012: 5
FWCI
2.38
Type
journal article
Language
en
Open access
closed
Locations
  • Development (publishedVersion)
  • PubMed (publishedVersion)
  • HAL (submittedVersion)
Funders
Agence Nationale de la Recherche (ANR-08-BLAN-0162)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Axon Guidance and Neuronal Signaling
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
  • Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
Keywords
ephrin-A5, adenosine A1 receptor, axon guidance, growth cone collapse, ATP release, adenosine signaling, optic tectum, chick embryo
MeSH terms
Engrailed 2 Protein, Adenosine Triphosphate, Animals, Chick Embryo, Fluorescent Antibody Technique, Microscopy, Fluorescence, Models, Biological, Nerve Tissue Proteins, Retina, Blotting, Western, Signal Transduction, Homeodomain Proteins, Growth Cones, Ephrin-A5, Proteomics, Receptor, Adenosine A1

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