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Localization of vasa protein to the Drosophila pole plasm is independent of its RNA-binding and helicase activities
Abstract
The Drosophila gene vasa encodes a DEAD-box protein, which is localized during early oogenesis to the perinuclear region of the nurse cells and later to the pole plasm at the posterior end of the oocyte. Posterior localization of vasa protein depends upon the functions of four genes: capu, spir, osk and stau. We have found that localization of vasa to the perinuclear nuage is abolished in most vas alleles, but is unaffected by mutations in four genes required upstream for its pole plasm localization. Thus localization of vasa to the nuage particles is independent of the pole plasm assembly pathway. Furthermore, electron-dense nuage particles are less abundant in the cytoplasm of nurse cells from vas mutants that fail to exhibit perinuclear localization, suggesting that the formation of the nuage depends upon vas function. Eight of nine vas point mutations cause codon substitutions in a region conserved among DEADbox genes. The proteins from two mutant alleles that retain the capacity to localize to the posterior pole of the oocyte, vasO14 and vasO11, are both severely reduced in RNAbinding and -unwinding activity as compared to the wildtype protein on a variety of RNA substrates including in vitro synthesized pole plasm RNAs. Initial recruitment of vasa to the pole plasm must consequently depend upon protein-protein interactions but, once localized, vasa must bind to RNA to mediate germ cell formation.
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Nott, T. J., Petsalaki, E., Farber, P., Jervis, D., Fussner, E., Plochowietz, A., Craggs, T. D., Bazett-Jones, D. P., Pawson, T., Forman-Kay, J. D., et al. (2015) · Molecular Cell · doi:10.1016/j.molcel.2015.01.013 · Cited by 1,803
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Rocak, S., & Linder, P. (2004) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1335 · Cited by 772
Xie, T., & Spradling, A. C. (1998) · Cell · doi:10.1016/s0092-8674(00)81424-5 · Cited by 668
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Tanaka, S. S., Toyooka, Y., Akasu, R., Katoh-Fukui, Y., Nakahara, Y., Suzuki, R., Yokoyama, M., & Noce, T. (2000) · Genes & Development · doi:10.1101/gad.14.7.841 · Cited by 629
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Py, B., Higgins, C. F., Krisch, H. M., & Carpousis, A. J. (1996) · Nature · doi:10.1038/381169a0 · Cited by 553
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Gray, N. K., & Wickens, M. (1998) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.14.1.399 · Cited by 503
Song, X., Wong, M. D., Kawase, E., Xi, R., Ding, B. C., McCarthy, J. J., & Xie, T. (2004) · Development · doi:10.1242/dev.01026 · Cited by 416
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Jacobsen, S. E., Running, M. P., & Meyerowitz, E. M. (1999) · Development · doi:10.1242/dev.126.23.5231 · Cited by 371
Lim, A. K., & Kai, T. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0701920104 · Cited by 369
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Wickens, M., Anderson, P., & Jackson, R. J. (1997) · Current Opinion in Genetics & Development · doi:10.1016/s0959-437x(97)80132-3 · Cited by 332
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Parsyan, A., Svitkin, Y., Shahbazian, D., Gkogkas, C., Lasko, P., Merrick, W. C., & Sonenberg, N. (2011) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3083 · Cited by 323
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de Cuevas, M., Lilly, M., & Spradling, A. (1997) · Annual Review of Genetics · doi:10.1146/annurev.genet.31.1.405 · Cited by 305
Shibata, N., Umesono, Y., Orii, H., Sakurai, T., Watanabe, K., & Agata, K. (1999) · Developmental Biology · doi:10.1006/dbio.1998.9130 · Cited by 275
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Khurana, J. S., Wang, J., Xu, J., Koppetsch, B. S., Thomson, T. C., Nowosielska, A., Li, C., Zamore, P. D., Weng, Z., & Theurkauf, W. E. (2011) · Cell · doi:10.1016/j.cell.2011.11.042 · Cited by 255
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Gustafson, E. A., & Wessel, G. M. (2010) · BioEssays · doi:10.1002/bies.201000001 · Cited by 173
Lasko, P. (2013) · Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms · doi:10.1016/j.bbagrm.2013.04.005 · Cited by 123
Self-citationAnne, J., Ollo, R., Ephrussi, A., & Mechler, B. M. (2007) · Development · doi:10.1242/dev.02687 · Cited by 78
Metadata
- Authors
- Liang, L., Diehl-Jones, W., & Lasko, P.
- Venue
- Development
- Publisher
- The Company of Biologists
- Published
- 1994-05-01
- Volume
- 120
- Issue
- 5
- Pages
- 1201-1211
- ISSN
- 0950-1991 (ISSN-L)
- 1477-9129
- OpenAlex
- W2120646730
- Cited by
- 343
- References
- 0
- FWCI
- 3.31
- Type
- journal article
- Language
- en
- Open access
- closed
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