Cited by 192Developmental Biology and Gene RegulationNeurogenesis and neuroplasticity mechanismsZebrafish Biomedical Research Applications

her4 , a zebrafish homologue of the Drosophila neurogenic gene E(spl) , is a target of NOTCH signalling

Takke, Christina, Dornseifer, Peter, Weizsäcker, Elisabeth v., Campos-Ortega, José A.·1999·Development

Abstract

her4 encodes a zebrafish bHLH protein of the HAIRY-E(SPL) family. The gene is transcribed in a complex pattern in the developing nervous system and in the hypoblast. During early neurogenesis, her4 expression domains include the regions of the neural plate from which primary neurons arise, suggesting that the gene is involved in directing their development. Indeed, misexpression of specific her4 variants leads to a reduction in the number of primary neurons formed. The amino-terminal region of HER4, including the basic domain, and the region between the putative helix IV and the carboxy-terminal tetrapeptide WRPW are essential for this effect, since HER4 variants lacking either of these regions are non-functional. However, the carboxy-terminal WRPW itself is dispensable. We have examined the interrelationships between deltaD, deltaA, notch1, her4 and neurogenin1 by means of RNA injections. her4 is involved in a regulatory feedback loop which modulates the activity of the proneural gene neurogenin, and as a consequence, of deltaA and deltaD. Activation of notch1 leads to strong activation of her4, to suppression of neurogenin transcription and, ultimately, to a reduction in the number of primary neurons. These results suggest that her4 acts as a target of notch-mediated signals that regulate primary neurogenesis.

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Crossref · 10.1242/dev.126.9.1811

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Authors
Takke, C., Dornseifer, P., Weizsäcker, E. V., & Campos-Ortega, J. A.
Affiliations
  • University of Cologne (DE)
  • Universität zu Köln Institut für Entwicklungsbiologie , , 50923 Köln, Germany
Venue
Development
Published
1999-05-01
Volume
126
Issue
9
Pages
1811-1821
ISSN
  • 0950-1991 (ISSN-L)
  • 1477-9129
OpenAlex
W2106017246
Cited by
192
References
61
Citations by year
2026: 3 · 2025: 4 · 2024: 10 · 2023: 10 · 2022: 2 · 2021: 1 · 2020: 7 · 2019: 8 · 2018: 2 · 2017: 2 · 2016: 6 · 2015: 5 · 2014: 8 · 2013: 10 · 2012: 3
FWCI
3.79
Type
journal article
Language
en
Open access
closed
Locations
  • Development (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
  • Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Zebrafish Biomedical Research Applications
Keywords
ErbB4, Notch signaling, primary neurogenesis, bHLH transcription factors, neurogenin1, proneural genes, δD, Notch1, hypoblast, neural plate, zebrafish, transcriptional feedback loop, ectopic expression
MeSH terms
Animals, DNA-Binding Proteins, Drosophila, Embryo, Nonmammalian, Embryonic Induction, Feedback, Membrane Proteins, Nerve Tissue Proteins, Nervous System, Neurons, Repressor Proteins, Transcription Factors, Transcription, Genetic, Zebrafish, Signal Transduction, Trans-Activators, Protein Structure, Secondary, Helix-Loop-Helix Motifs, Homeodomain Proteins, Gene Expression Regulation, Developmental, Insect Proteins, Drosophila Proteins, Xenopus Proteins, Zebrafish Proteins, Basic Helix-Loop-Helix Proteins, Receptors, Notch, LIM-Homeodomain Proteins

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