Endocytic trafficking in actively resorbing osteoclasts

Stenbeck, Gudrun; Horton, Michael A.

doi:10.1242/jcs.00935

Cited by 79Bone Metabolism and DiseasesCellular transport and secretionAutophagy in Disease and Therapy

The DOI matches its Crossref record; 34 of the 34 references it cites were found in a registry or an index.

Endocytic trafficking in actively resorbing osteoclasts

Stenbeck, Gudrun, Horton, Michael A.·2004·Journal of Cell Science

DOI 10.1242/jcs.00935

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Abstract

Endocytosis and the subsequent intracellular trafficking of the endocytosed material are important determinants of cellular function. Osteoclasts, cells of the monocyte/macrophage family, are specialized for the internalization and processing of bone matrix. Transcytosis of endocytosed material has been observed in osteoclasts but the precise mechanism controlling this process is unclear. Here, we investigate the regulation of these trafficking events. To establish the directionality and kinetics of trafficking events in resorbing osteoclasts, we devised a system using fluorescent low-molecular-weight markers as probes to follow the route taken by the digested bone matrix. We demonstrate that this route is largely distinct from the pathway followed by proteins taken up by receptor-mediated endocytosis at the basolateral plasma membrane. Endocytosis and transcytosis from the ruffled border are fast processes, with a half-life of the endocytosed material inside the cells of 22 minutes. We demonstrate the crucial role of the microtubule network in transport from the ruffled-border area and provide evidence for a role of the cytoskeleton in the overall efficacy of trafficking. Moreover, we analyse the effect of the V-ATPase inhibitor bafilomycin A1 on endocytic uptake, which gives insight into the pH-dependent regulation of membrane trafficking and resorption in osteoclasts.

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After endocytosis, products derived from matrix degradation are transported through the cell within vesicles for further intracellular degradation and exocytosed on the opposite site through the secretory domain at the basolateral membrane.
“After endocytosis, the products derived from matrix degradation are transported through the cell within vesicles for further intracellular degradation and exocytosed on the opposite site through the secretory domain at the basolateral membrane.189”
From Bone remodeling: an operational process ensuring survival and bone mechanical competence (2022) · cites this paper as [189]

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Authors: Stenbeck, G., & Horton, M. A.
Affiliations: University College London (GB)
Corresponding author: Stenbeck, Gudrun
ORCID: Horton, Michael A.
Venue: Journal of Cell Science
Publisher: The Company of Biologists
Published: 2004-02-22
Volume: 117
Issue: 6
Pages: 827-836
ISSN: 0021-9533 (ISSN-L)
1477-9137
DOI: 10.1242/jcs.00935
OpenAlex: W2093719268
Cited by: 79
References: 34
Citations by year: 2025: 2 · 2023: 2 · 2022: 3 · 2021: 4 · 2020: 4 · 2019: 5 · 2018: 2 · 2017: 4 · 2016: 1 · 2015: 8 · 2014: 4 · 2013: 6 · 2012: 2
FWCI: 1.30
Type: journal article
Language: en
Open access: closed
Locations: Journal of Cell Science (publishedVersion)
PubMed (publishedVersion)
UCL Discovery (University College London) (submittedVersion)
HighWire Press Open Archive (submittedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Autophagy in Disease and Therapy
Keywords: osteoclasts, endocytosis, endosomal trafficking, transcytosis, bone resorption, ruffled border, microtubule cytoskeleton, cytoskeleton, V-ATPase, bafilomycin A1, membrane trafficking, fluorescent markers
MeSH terms: Adenosine Triphosphatases, Animals, Biological Transport, Active, Bone Resorption, Cell Compartmentation, Cells, Cultured, Cytoskeleton, Dextrans, Endocytosis, Enzyme Inhibitors, Osteoclasts, Phalloidine, Rabbits, Biomarkers, Macrolides

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Endocytic trafficking in actively resorbing osteoclasts

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