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Cited by 104Bone Metabolism and DiseasesCellular transport and secretionExtracellular vesicles in disease

Intracellular membrane trafficking in bone resorbing osteoclasts

Mulari, Mika, Vääräniemi, Jukka, Väänänen, H. Kalervo·2003·Microscopy Research and Technique
DOI 10.1002/jemt.10371
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Abstract

There is ample evidence now that the two major events in bone resorption, namely dissolution of hydroxyapatite and degradation of the organic matrix, are performed by osteoclasts. The resorption cycle involves several specific cellular activities, where intracellular vesicular trafficking plays a crucial role. Although details of these processes started to open up only recently, it is clear that vesicular trafficking is needed in several specific steps of osteoclast functioning. Several plasma membrane domains are formed during the polarization of the resorbing cells. Multinucleated osteoclasts create a tight sealing to the extracellular matrix as a first indicator of their resorption activity. Initial steps of the sealing zone formation are α v β 3‐integrin mediated, but the final molecular interaction(s) between the plasma membrane and mineralized bone matrix is still unknown. A large number of acidic intracellular vesicles then fuse with the bone‐facing plasma membrane to form a ruffled border membrane, which is the actual resorbing organelle. The formation of a ruffled border is regulated by a small GTP‐binding protein, rab7, which indicates the late endosomal character of the ruffled border membrane. Details of specific membrane transport processes in the osteoclasts, e.g., the formation of the sealing zone and transcytosis of bone degradation products from the resorption lacuna to the functional secretory domain remain to be clarified. It is tempting to speculate that specific features of vesicular trafficking may offer several potential new targets for drug therapy of bone diseases. Microsc. Res. Tech. 61:496–503, 2003. © 2003 Wiley‐Liss, Inc.

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  • During bone remodeling osteoclasts polarize into four membrane domains: the sealing zone, ruffled border, basolateral domain, and functional secretory domain.

    “During bone remodeling osteoclasts polarize; then, four types of osteoclast membrane domains can be observed: the sealing zone and ruffled border that are in contact with the bone matrix (Figures 4(b) and 4(d)), as well as the basolateral and functional secretory domains, which are not in contact with the bone matrix [94, 95].”

    From Biology of Bone Tissue: Structure, Function, and Factors That Influence Bone Cells (2015) · cites this paper as [94]

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Authors
Mulari, M., Vääräniemi, J., & Väänänen, H. K.
Affiliations
University of Turku (FI)
Corresponding author
Väänänen, H. Kalervo
Venue
Microscopy Research and Technique
Publisher
Wiley
Published
2003-07-18
Volume
61
Issue
6
Pages
496-503
ISSN
  • 1059-910X (ISSN-L)
  • 1097-0029
DOI
10.1002/jemt.10371
OpenAlex
W1974688989
Cited by
104
References
85
Citations by year
2025: 4 · 2024: 3 · 2023: 7 · 2022: 3 · 2021: 5 · 2020: 4 · 2019: 7 · 2018: 3 · 2017: 2 · 2016: 3 · 2015: 3 · 2014: 6 · 2013: 1 · 2012: 6
FWCI
0.39
Type
journal article
Language
en
Open access
closed
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations
  • Microscopy Research and Technique (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
Keywords
osteoclasts, bone resorption, intracellular membrane trafficking, vesicle trafficking, sealing zone, ruffled border, alpha v beta 3 integrin, Rab7, late endosomes, transcytosis, hydroxyapatite dissolution, bone matrix degradation, plasma membrane domains
MeSH terms
Animals, Bone Matrix, Bone Resorption, Cell Compartmentation, Humans, Intracellular Membranes, Osteoclasts, Cell Polarity, Protein Transport

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