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Cited by 454

The DOI matches its Crossref record; 42 of the 42 references it cites were found in a registry or an index.

Induction of heat shock proteins in B-cell exosomes

Open access
Clayton, Aled, Turkes, Attilla, Navabi, Hossein, Mason, Malcolm D., Tabi, Zsuzsanna·2005·Journal of Cell Science
DOI 10.1242/jcs.02494
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Abstract

Exosomes are nanometer-sized vesicles secreted by a diverse range of live cells that probably have physiological roles in modulating cellular immunity. The extracellular factors that regulate the quantity and phenotype of exosomes produced are poorly understood, and the properties of exosomes that dictate their immune functions are not yet clear. We investigated the effect of cellular stress on the exosomes produced by B-lymphoblastoid cell lines. Under steady-state conditions, the exosomes were positive for hsp27, hsc70, hsp70 and hsp90, and other recognised exosome markers such as MHC class I, CD81, and LAMP-2. Exposing cells to heat stress (42°C for up to 3 hours), resulted in a marked increase in these heat shock proteins (hsps), while the expression of other stress proteins such as hsp60 and gp96 remained negative, and other exosome markers remained unchanged. Stress also triggered a small increase in the quantity of exosomes produced [with a ratio of 1.245±0.07 to 1 (mean±s.e.m., n=20) of 3-hour-stress-exosomes to control-exosomes]. Flow-cytometric analysis of exosome-coated beads and immuno-precipitation of intact exosomes demonstrated that hsps were located within the exosome lumen, and not present at the exosome-surface, suggesting that such exosomes may not interact with target cells through cell-surface hsp-receptors. Functional studies further supported this finding, in that exosomes from control or heat-stressed B cells did not trigger dendritic cell maturation, assessed by analysis of dendritic-cell-surface phenotype, and cytokine secretion profile. Our findings demonstrate that specific alterations in exosome phenotype are a hitherto unknown component of the cellular response to environmental stress and their extracellular function does not involve the direct activation of dendritic cells.

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Metadata

Authors
Clayton, A., Turkes, A., Navabi, H., Mason, M. D., & Tabi, Z.
Venue
Journal of Cell Science
Publisher
The Company of Biologists
Published
2005-08-15
Volume
118
Issue
16
Pages
3631-3638
ISSN
  • 0021-9533 (ISSN-L)
  • 1477-9137
DOI
10.1242/jcs.02494
OpenAlex
W2111545764
Cited by
454
References
42
FWCI
7.14
Type
journal article
Language
en
Open access
hybrid

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