The DOI matches its Crossref record; 142 of the 142 references it cites were found in a registry or an index.
Micropatterning as a tool to decipher cell morphogenesis and functions
Abstract
In situ, cells are highly sensitive to geometrical and mechanical constraints from their microenvironment. These parameters are, however, uncontrolled under classic culture conditions, which are thus highly artefactual. Micro-engineering techniques provide tools to modify the chemical properties of cell culture substrates at sub-cellular scales. These can be used to restrict the location and shape of the substrate regions, in which cells can attach, so-called micropatterns. Recent progress in micropatterning techniques has enabled the control of most of the crucial parameters of the cell microenvironment. Engineered micropatterns can provide a micrometer-scale, soft, 3-dimensional, complex and dynamic microenvironment for individual cells or for multi-cellular arrangements. Although artificial, micropatterned substrates allow the reconstitution of physiological in situ conditions for controlled in vitro cell culture and have been used to reveal fundamental cell morphogenetic processes as highlighted in this review. By manipulating micropattern shapes, cells were shown to precisely adapt their cytoskeleton architecture to the geometry of their microenvironment. Remodelling of actin and microtubule networks participates in the adaptation of the entire cell polarity with respect to external constraints. These modifications further impact cell migration, growth and differentiation.
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“Among the most commonly used natural polymers are hyaluronic acid, gelatin, collagen, and chondroitin sulfate (14, 108).”
From Modeling physiological events in 2D vs. 3D cell culture (2017) · cites this paper as [108]
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Modeling physiological events in 2D vs. 3D cell culture
Duval, K., Grover, H., Han, L.-H., Mou, Y., Pegoraro, A. F., Fredberg, J., & Chen, Z. (2017) · Physiology · doi:10.1152/physiol.00036.2016 · Cited by 1,849
Deconstructing the third dimension – how 3D culture microenvironments alter cellular cues
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Cao, H., Duan, L., Zhang, Y., Cao, J., & Zhang, K. (2021) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-021-00830-x · Cited by 1,380
A Review of Cell Adhesion Studies for Biomedical and Biological Applications
Khalili, A. A., & Ahmad, M. R. (2015) · International Journal of Molecular Sciences · doi:10.3390/ijms160818149 · Cited by 999
Three-dimensional culture systems in cancer research: Focus on tumor spheroid model
Nath, S., & Devi, G. R. (2016) · Pharmacology & Therapeutics · doi:10.1016/j.pharmthera.2016.03.013 · Cited by 919
Metadata
- Authors
- Théry, M.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- Corresponding author
- Théry, Manuel
- ORCID
- Théry, Manuel
- Venue
- Journal of Cell Science
- Publisher
- The Company of Biologists
- Published
- 2010-12-15
- Volume
- 123
- Issue
- 24
- Pages
- 4201-4213
- ISSN
- 0021-9533 (ISSN-L)
- 1477-9137
- OpenAlex
- W2057917964
- Cited by
- 730
- References
- 142
- Citations by year
- 2026: 22 · 2025: 22 · 2024: 19 · 2023: 45 · 2022: 55 · 2021: 49 · 2020: 66 · 2019: 58 · 2018: 65 · 2017: 54 · 2016: 50 · 2015: 53 · 2014: 53 · 2013: 63 · 2012: 43
- FWCI
- 3.41
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Cell Science (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- HighWire Press Open Archive (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Cell Image Analysis Techniques
- Keywords
- micropatterning, cell morphogenesis, cell microenvironment, substrate geometry, cell mechanics, actin cytoskeleton, microtubules, cell polarity, cell migration, cell differentiation, cell growth, microengineering, 3D cell culture
- MeSH terms
- Animals, Cell Differentiation, Cells, Humans, Cell Polarity, Cell Culture Techniques, Cell Shape, Cell Proliferation
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