The DOI matches its Crossref record; 42 of the 42 references it cites were found in a registry or an index.
In vitro differentiation of embryonic stem cells into glial cells and functional neurons
Abstract
Mouse embryonic stem cells were induced to differentiate in culture with retinoic acid. Putative precursors of neurons and glial cells (nestin-positive cells) were clearly identified as early as three days after the onset of differentiation. At day 6, neuron-like cells could be clearly identified, either as isolated cells or as cellular networks. Some of these cells were positive for astrocyteor oligodendrocyte-specific antigens (GFAP or O4 antigens, respectively). Other cells were positive for neuron-specific antigens (cytoskeleton proteins MAP2, MAP5 and NF200, as well as synaptophysin). Some neuronal-like cells were also positive for acetylcholinesterase activity or glutamic acid decarboxylase expression, indicating that ES cells could differentiate into GABAergic and possibly cholinergic neurons. Electrophysiological analyses performed in voltage clamp conditions showed that cell membranes contained voltagedependent channels. Overshooting action potentials could be triggered by current injection. Taken together, these data provide evidence that embryonic stem cells can differentiate first into neuron-glia progenitors, and later into glial cells and functional neurons, in vitro. This technique provides an unique system to study early steps of neuronal differentiation in vitro.
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Cites
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Cited by
Differentiation of mouse embryonic stem cells into a defined neuronal lineage
Bibel, M., Richter, J., Schrenk, K., Tucker, K. L., Staiger, V., Korte, M., Goetz, M., & Barde, Y.-A. (2004) · Nature Neuroscience · doi:10.1038/nn1301 · Cited by 450
Differentiation of embryonic stem cells into adipocytes in vitro
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Scalable Stirred-Suspension Bioreactor Culture of Human Pluripotent Stem Cells
Kehoe, D. E., Jing, D., Lock, L. T., & Tzanakakis, E. S. (2010) · Tissue Engineering Part A · doi:10.1089/ten.tea.2009.0454 · Cited by 264
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Metadata
- Authors
- Fraichard, A., Chassande, O., Bilbaut, G., Dehay, C., Savatier, P., & Samarut, J.
- Venue
- Journal of Cell Science
- Publisher
- The Company of Biologists
- Published
- 1995-10-01
- Volume
- 108
- Issue
- 10
- Pages
- 3181-3188
- ISSN
- 0021-9533 (ISSN-L)
- 1477-9137
- OpenAlex
- W2135668873
- Cited by
- 420
- References
- 42
- FWCI
- 3.79
- Type
- journal article
- Language
- en
- Open access
- closed
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- Fraichard, A., Chassande, O., Bilbaut, G., Dehay, C., Savatier, P., & Samarut, J. (1995). In vitro differentiation of embryonic stem cells into glial cells and functional neurons. Journal of Cell Science. https://doi.org/10.1242/jcs.108.10.3181
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
