Cited by 1,683CRISPR and Genetic EngineeringAnimal Genetics and ReproductionPluripotent Stem Cells Research

Disruption of the proto-oncogene int-2 in mouse embryo-derived stem cells: A general strategy for targeting mutations to non-selectable genes

Mansour, Suzanne L., Thomas, Kirk R., Capecchi, Mario R.·1988·Nature

Abstract

Gene targeting--homologous recombination of DNA sequences residing in the chromosome with newly introduced DNA sequences--in mouse embryo-derived stem cells promises to provide a means to generate mice of any desired genotype. We describe a positive nd negative selection procedure that enriches 2,000-fold for those cells that contain a targeted mutation. The procedure was applied to the isolation of hprt- and int-2- mutants, but it should be applicable to any gene.

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Crossref · 10.1038/336348a0

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15 of 15 references verified0 citation errors · 0 not checkable

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  • Due to the extremely low frequency of homologous recombination, this technique cannot be used for human gene therapy.

    “However, due to the extremely low frequency of HR, this technique cannot be used for human gene therapy.”

    From Newer Gene Editing Technologies toward HIV Gene Therapy (2013) · cites this paper as [15]

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Authors
Mansour, S. L., Thomas, K. R., & Capecchi, M. R.
Affiliations
Howard Hughes Medical Institute (US)
Venue
Nature
Published
1988-11
Volume
336
Issue
6197
Pages
348-352
ISSN
  • 0028-0836 (ISSN-L)
  • 1476-4687
OpenAlex
W1983237714
Cited by
1,683
References
15
Citations by year
2026: 2 · 2025: 18 · 2024: 9 · 2023: 14 · 2022: 14 · 2021: 16 · 2020: 12 · 2019: 18 · 2018: 15 · 2017: 15 · 2016: 26 · 2015: 24 · 2014: 26 · 2013: 29 · 2012: 32
FWCI
21.48
Type
journal article
Language
en
Open access
closed
Locations
  • Nature (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Animal Genetics and Reproduction
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
Keywords
gene targeting, homologous recombination, positive and negative selection, knockout mice
MeSH terms
Animals, Clone Cells, Mice, Transgenic, Mutation, Proto-Oncogenes, Stem Cells, Transfection, Restriction Mapping, Mice

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