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Cytoplasmic accumulation of cdc25B phosphatase in mitosis triggers centrosomal microtubule nucleation in HeLa cells
Abstract
The formation of the mitotic spindle is an essential prerequisite for successful mitosis. The dramatic changes in the level of microtubule (Mt) nucleation at the centrosomes and Mt dynamics that occur in prophase are presumed to be initiated through the activity of cdc2/cyclin B. Here we present data that the cdc25B isoform functions to activate the cytoplasmic pool of cdc2/cyclin B responsible for these events. In contrast to cdc25C, cdc25B is present at low levels in HeLa cells during interphase, but sharply increases in prophase, when cdc25B accumulation in the cytoplasm correlates with prophase spindle formation. Overexpression of wild type and dominant negative mutants of cdc25B and cdc25C shows that prophase Mt nucleation is a consequence of cytoplasmic cdc25B activity, and that cdc25C regulates nuclear G2/M events. Our data also suggest that the functional status of the centrosome can regulate nuclear mitotic events.
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Citations
Cited by
Partial inhibition of Cdk1 in G2phase overrides the SAC and decouples mitotic events
McCloy, R. A., Rogers, S., Caldon, C. E., Lorca, T., Castro, A., & Burgess, A. (2014) · Cell Cycle · doi:10.4161/cc.28401 · Cited by 853
Cell cycle regulation by the Cdc25 phosphatase family
Nilsson, I., & Hoffmann, I. (2000) · Progress in Cell Cycle Research · doi:10.1007/978-1-4615-4253-7_10 · Cited by 450
Regulating mammalian checkpoints through Cdc25 inactivation
Donzelli, M., & Draetta, G. F. (2003) · EMBO reports · doi:10.1038/sj.embor.embor887 · Cited by 438
The when and wheres of CDC25 phosphatases
Boutros, R., Dozier, C., & Ducommun, B. (2006) · Current Opinion in Cell Biology · doi:10.1016/j.ceb.2006.02.003 · Cited by 414
Chk1 mediates S and G2 arrests through Cdc25A degradation in response to DNA-damaging agents
Xiao, Z., Chen, Z., Gunasekera, A. H., Sowin, T. J., Rosenberg, S. H., Fesik, S., & Zhang, H. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m300229200 · Cited by 388
Metadata
- Authors
- Gabrielli, B. G., Souza, C. P. C. D., Tonks, I. D., Clark, J. M., Hayward, N. K., & Ellem, K. A. O.
- Venue
- Journal of Cell Science
- Publisher
- The Company of Biologists
- Published
- 1996-05-01
- Volume
- 109
- Issue
- 5
- Pages
- 1081-1093
- ISSN
- 0021-9533 (ISSN-L)
- 1477-9137
- OpenAlex
- W1925896202
- Cited by
- 236
- References
- 0
- FWCI
- 3.91
- Type
- journal article
- Language
- en
- Open access
- closed
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- The paper
- Gabrielli, B. G., Souza, C. P. C. D., Tonks, I. D., Clark, J. M., Hayward, N. K., & Ellem, K. A. O. (1996). Cytoplasmic accumulation of cdc25B phosphatase in mitosis triggers centrosomal microtubule nucleation in HeLa cells. Journal of Cell Science. https://doi.org/10.1242/jcs.109.5.1081
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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