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Proteinase-activated receptor-2: expression by human neutrophils
Abstract
Neutrophils were shown to express the proteinaseactivated receptor-2 (PAR-2), a seven transmembrane domain receptor, which is activated by cleavage by trypsin. Granulocytes from 14 donors stained positively for PAR-2 with affinity-purified rabbit antibodies raised against a peptide corresponding to the trypsin cleavage site of human PAR-2. Neutrophil activation in response to a receptor activating peptide (RAP) varied between donors. RAP (Ser-Leu-Ile-Gly-Lys-Val-NH2) alone induced an increase in the forward and side light scatter after 5-10 minutes and a small increase in the expression of the activation molecule CD11b. The increased expression of CD11b induced by RAP was markedly enhanced by priming the neutrophils with a low concentration (1 nM) of formyl-Leu-Met-Phe. Trypsin and RAP also induced an increase in intracellular calcium, but there were large variations in the magnitude of responses between donors also in this assay. The effects of RAP in the different assays were specific; acetylated RAP was completely without activity.
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Cited by
Protease-Activated Receptors: Contribution to Physiology and Disease
OSSOVSKAYA, V. S., & BUNNETT, N. W. (2004) · Physiological Reviews · doi:10.1152/physrev.00028.2003 · Cited by 1,078
Proteinase-Activated Receptors
Macfarlane, S. R., Seatter, M. J., Kanke, T., Hunter, G. D., & Plevin, R. (2001) · Pharmacological Reviews · doi:10.1016/s0031-6997(24)01493-5 · Cited by 641
Protease-activated receptors in inflammation, neuronal signaling and pain
Vergnolle, N., Wallace, J. L., Bunnett, N. W., & Hollenberg, M. D. (2001) · Trends in Pharmacological Sciences · doi:10.1016/s0165-6147(00)01634-5 · Cited by 369
Lourbakos, A., Chinni, C., Thompson, P., Potempa, J., Travis, J., Mackie, E. J., & Pike, R. N. (1998) · FEBS Letters · doi:10.1016/s0014-5793(98)01036-9 · Cited by 171
Self-citationProtease-activated receptors in health and disease
Peach, C. J., Edgington-Mitchell, L. E., Bunnett, N. W., & Schmidt, B. L. (2023) · Physiological Reviews · doi:10.1152/physrev.00044.2021 · Cited by 116
Metadata
- Authors
- Howells, G. L., Macey, M. G., Chinni, C., Hou, L., Fox, M. T., Harriott, P., & Stone, S. R.
- Venue
- Journal of Cell Science
- Publisher
- The Company of Biologists
- Published
- 1997-04-01
- Volume
- 110
- Issue
- 7
- Pages
- 881-887
- ISSN
- 0021-9533 (ISSN-L)
- 1477-9137
- OpenAlex
- W2170147946
- Cited by
- 158
- References
- 0
- FWCI
- 3.15
- Type
- journal article
- Language
- en
- Open access
- closed
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- Howells, G. L., Macey, M. G., Chinni, C., Hou, L., Fox, M. T., Harriott, P., & Stone, S. R. (1997). Proteinase-activated receptor-2: expression by human neutrophils. Journal of Cell Science. https://doi.org/10.1242/jcs.110.7.881
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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