The DOI matches its Crossref record; 63 of the 63 references it cites were found in a registry or an index.
Inhibition of clathrin-coated pit assembly by an Eps15 mutant
Abstract
Recent data have shown that Eps15, a newly identified component of clathrin-coated pits constitutively associated with the AP-2 complex, is required for receptor-mediated endocytosis. However, its precise function remains unknown. Interestingly, Eps15 contains three EH (Eps15-Homology) domains also found in proteins required for the internalization step of endocytosis in yeast. Results presented here show that EH domains are required for correct coated pit targeting of Eps15. Furthermore, when cells expressed an Eps15 mutant lacking EH domains, the plasma membrane punctate distribution of both AP-2 and clathrin was lost, implying the absence of coated pits. This was further confirmed by the fact that dynamin, a GTPase found in coated pits, was homogeneously redistributed on the plasma membrane and that endocytosis of transferrin, a specific marker of clathrin-dependent endocytosis, was strongly inhibited. Altogether, these results strongly suggest a role for Eps15 in coated pit assembly and more precisely a role for Eps15 in the docking of AP-2 onto the plasma membrane. This hypothesis is supported by the fact that a GFP fusion protein encoding the ear domain of α-adaptin, the AP-2 binding site for Eps15, was efficiently targeted to plasma membrane coated pits.
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Cites
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Dynamin-mediated Internalization of Caveolae
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Cited by
Routes and mechanisms of extracellular vesicle uptake
Mulcahy, L. A., Pink, R. C., & Carter, D. R. F. (2014) · Journal of Extracellular Vesicles · doi:10.3402/jev.v3.24641 · Cited by 2,832
Molecular mechanism and physiological functions of clathrin-mediated endocytosis
McMahon, H. T., & Boucrot, E. (2011) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3151 · Cited by 2,223
Clathrin-independent endocytosis of ubiquitinated cargos
Sigismund, S., Woelk, T., Puri, C., Maspero, E., Tacchetti, C., Transidico, P., Di Fiore, P. P., & Polo, S. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0409817102 · Cited by 739
Accessory factors in clathrin-dependent synaptic vesicle endocytosis
Slepnev, V. I., & De Camilli, P. (2000) · Nature Reviews Neuroscience · doi:10.1038/35044540 · Cited by 517
Ultrastructural identification of uncoated caveolin-independent early endocytic vehicles
Kirkham, M., Fujita, A., Chadda, R., Nixon, S. J., Kurzchalia, T. V., Sharma, D. K., Pagano, R. E., Hancock, J. F., Mayor, S., & Parton, R. G. (2005) · The Journal of Cell Biology · doi:10.1083/jcb.200407078 · Cited by 439
Metadata
- Authors
- Benmerah, A., Bayrou, M., Cerf-Bensussan, N., & Dautry-Varsat, A.
- Venue
- Journal of Cell Science
- Publisher
- The Company of Biologists
- Published
- 1999-05-01
- Volume
- 112
- Issue
- 9
- Pages
- 1303-1311
- ISSN
- 0021-9533 (ISSN-L)
- 1477-9137
- OpenAlex
- W2155259994
- Cited by
- 423
- References
- 63
- FWCI
- 4.78
- Type
- journal article
- Language
- en
- Open access
- closed
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- Benmerah, A., Bayrou, M., Cerf-Bensussan, N., & Dautry-Varsat, A. (1999). Inhibition of clathrin-coated pit assembly by an Eps15 mutant. Journal of Cell Science. https://doi.org/10.1242/jcs.112.9.1303
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