The effect of inhalational anaesthetics on the swimming velocity of Tetrahymena pyriformis
Abstract
The effect of 6 inhalational anaesthetics on speed of swimming (produced by ciliary movement) has been studied in Tetrahymena pyriformis. There was no evidence of stimulation at low dose levels and higher levels caused rapid, reversible, dose-dependent reduction in swimming velocity. The concentrations of anaesthetics which depressed motility by 50 % were of the same order as those required for anaesthesia in mammals, except in the case of cyclopropane, for which the required level was 4 times higher than the anaesthetic level. Correlation with lipid solubility was not as close as is the case for narcotic concentration. Oxygen consumption was reduced with increasing amounts of halothane in parallel with the reduction in swimming velocity. Halothane produced deciliation of Tetrahymena at about 10 times the anaesthetic dose for man; regrowth of cilia took place within 4 h of withdrawal of the drug. There were no changes in the ultrastructure of the cilia, basal bodies and associated microtubular systems at levels of halothane sufficient to stop cilial beat. At higher concentrations deciliation occurred immediately distal to the axosome and there was variable swelling of the mitochondria.
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Volatile anesthetics act on species across the evolutionary tree, not only on human patients.
“However, volatile anesthetics exert actions not only on human patients, but on species spanning the evolutionary tree of life [1–8] (Figure 1).”
From The Biology of General Anesthesia from Paramecium to Primate (2019) · cites this paper as [1]
Cites
THE USE OF LEAD CITRATE AT HIGH pH AS AN ELECTRON-OPAQUE STAIN IN ELECTRON MICROSCOPY
Reynolds, E. S. (1963) · The Journal of Cell Biology · doi:10.1083/jcb.17.1.208 · Cited by 24,938 · see citing works
IMPROVEMENTS IN EPOXY RESIN EMBEDDING METHODS
Luft, J. H. (1961) · The Journal of Cell Biology · doi:10.1083/jcb.9.2.409 · Cited by 10,243 · see citing works
Staining of Tissue Sections for Electron Microscopy with Heavy Metals
Watson, M. L. (1958) · The Journal of Cell Biology · doi:10.1083/jcb.4.4.475 · Cited by 4,115 · see citing works
Dynein: A Protein with Adenosine Triphosphatase Activity from Cilia
Gibbons, I. R., & Rowe, A. J. (1965) · Science · doi:10.1126/science.149.3682.424 · Cited by 594 · see citing works
Statistical Method in Biological Assay.
Armitage, P., & Finney, D. J. (1965) · Journal of the Royal Statistical Society Series A (General) · doi:10.2307/2343446 · Cited by 578 · see citing works
Biochemistry and Physiology of Protozoa
Warren, L. G. (1980) · American Journal of Tropical Medicine and Hygiene · doi:10.4269/ajtmh.1980.29.1020 · Cited by 564 · see citing works
STUDIES ON THE PROTEIN COMPONENTS OF CILIA FROM TETRAHYMENA PYRIFORMIS
Gibbons, I. R. (1963) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.50.5.1002 · Cited by 292 · see citing works
SOLUBILITY COEFFICIENTS FOR INHALED ANAESTHETICS FOR WATER, OIL AND BIOLOGICAL MEDIA
Steward, A. N., ALLOTT, P. R., Cowles, A. L., & MAPLESON, W. W. (1973) · British Journal of Anaesthesia · doi:10.1093/bja/45.3.282 · Cited by 242 · see citing works
Binding of Xenon to Sperm Whale Myoglobin
SCHOENBORN, B. P., WATSON, H. C., & Kendrew, J. C. (1965) · Nature · doi:10.1038/207028a0 · Cited by 241 · see citing works
Allen, R. D. (1967) · The Journal of Protozoology · doi:10.1111/j.1550-7408.1967.tb02042.x · Cited by 237 · see citing works
The Biochemistry and Physiology of Tetrahymena
(1972) · Elsevier eBooks · doi:10.1016/b978-0-123-48350-8.x5001-8 · Cited by 213 · see citing works
COLCHICINE MITOSIS, CHROMOSOME CONTRACTION, NARCOSIS AND PROTEIN CHAIN FOLDING
ÖSTERGREN, G. (2010) · Hereditas · doi:10.1111/j.1601-5223.1944.tb02739.x · Cited by 189 · see citing works
THE MECHANISM OF C-MITOTIC ACTION OBSERVATIONS ON THE NAPHTHALENE SERIES
LEVAN, A., & ÖSTERGREN, G. (2010) · Hereditas · doi:10.1111/j.1601-5223.1943.tb03297.x · Cited by 115 · see citing works
Nilsson, L., & Siesjö, B. K. (1970) · Acta Physiologica Scandinavica · doi:10.1111/j.1748-1716.1970.tb04787.x · Cited by 72 · see citing works
The effect of inhalational anaesthetics on the microtubular system in Actinosphaerium nucleofilum
ALLISON, A. C., HULANDS, G. H., NUNN, J. F., Kitching, J. A., & Macdonald, A. C. (1970) · Journal of Cell Science · doi:10.1242/jcs.7.2.483 · Cited by 61 · see citing works
Self-citationTHE CILIARY MOVEMENT OF THE TRACHEA STUDIED IN VITRO.
Hill, L. (1928) · The Lancet · doi:10.1016/s0140-6736(00)77722-1 · Cited by 61 · see citing works
Effect of Anaesthetics on Emission of Light by Luminous Bacteria
White, D., & Dundas, C. (1970) · Nature · doi:10.1038/226456a0 · Cited by 51 · see citing works
Reversible alteration of the structure of globular proteins by anesthetic agents.
Balasubramanian, D., & Wetlaufer, D. B. (1966) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.55.4.762 · Cited by 50 · see citing works
Effects of Diethyl Ether and Halothane on Firefly Luciferin Bioluminescence
Ueda, I. (1965) · Anesthesiology · doi:10.1097/00000542-196509000-00003 · Cited by 50 · see citing works
THE ANAESTHETIC PRESSURES OF CERTAIN FLUORINE-CONTAINING GASES
Miller, K. W., Paton, W. D. M., & Smith, E. B. (1967) · British Journal of Anaesthesia · doi:10.1093/bja/39.12.910 · Cited by 37 · see citing works
Anesthetic Potencies of Sulfur Hexafluoride, Carbon Tetrafluoride, Chloroform and Ethrane in Dogs
Eger, E. I., Lundgren, C. E. G., Miller, S. L., & Stevens, W. C. (1969) · Anesthesiology · doi:10.1097/00000542-196902000-00003 · Cited by 35 · see citing works
ARREST OF MITOSIS BY HALOTHANE
Nunn, J. F., Lovis, J. D., & KIMBALL, K. L. (1971) · British Journal of Anaesthesia · doi:10.1093/bja/43.6.524 · Cited by 34 · see citing works
Self-citationPORTABLE INTERFERENCE REFRACTOMETERS IN ANAESTHESIA
HULANDS, G. H., & Nunn, J. F. (1970) · British Journal of Anaesthesia · doi:10.1093/bja/42.12.1051 · Cited by 34 · see citing works
Self-citationLeçons sur les propriétés des tissus vivants
Bernard, C., & Alglave, É. (1866) · doi:10.5962/bhl.title.3830 · Cited by 29 · see citing works
Fine structure changes during chloral hydrate deciliation of Paramecium caudatum
Kennedy, J. R., & Brittingham, E. (1968) · Journal of Ultrastructure Research · doi:10.1016/s0022-5320(68)90039-7 · Cited by 28 · see citing works
Fink, B. R. (1968) · Cited by 18 · see citing works
Effects of General Anaesthetics on Na+ Transport in Human Red Cells
Halsey, M. J., Smith, E. B., & WOOD, T. E. (1970) · Nature · doi:10.1038/2251151a0 · Cited by 16 · see citing works
PROCEEDINGS OF THE ANAESTHETIC RESEARCH SOCIETY EDINBURGH MEETING: JUNE 23, 1973
Grant, C. J., Powell, J., & RADFORD, S. G. (1973) · British Journal of Anaesthesia · doi:10.1093/bja/45.8.923 · Cited by 3 · see citing works
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The Biology of General Anesthesia from Paramecium to Primate
Kelz, M. B., & Mashour, G. A. (2019) · Current Biology · doi:10.1016/j.cub.2019.09.071 · Cited by 151 · see citing works
Tetrahymena in Cytotoxicology: with special reference to effects of heavy metals and selected drugs
Nilsson, J. R. (1989) · European Journal of Protistology · doi:10.1016/s0932-4739(89)80074-4 · Cited by 128 · see citing works
Issues in the Design and Interpretation of Minimum Alveolar Anesthetic Concentration (MAC) Studies
Sonner, J. M. (2002) · Anesthesia & Analgesia · doi:10.1213/00000539-200209000-00021 · Cited by 101 · see citing works
Molecular Mechanisms of Drug Action: An Emerging View
Sonner, J. M., & Cantor, R. S. (2013) · Annual Review of Biophysics · doi:10.1146/annurev-biophys-083012-130341 · Cited by 79 · see citing works
Steffey, E. P., Mama, K. R., & Brosnan, R. J. (2015) · doi:10.1002/9781119421375.ch16 · Cited by 74 · see citing works
A hypothesis on the origin and evolution of the response to inhaled anesthetics
Sonner, J. M. (2008) · Anesthesia & Analgesia · doi:10.1213/ane.0b013e31817ee684 · Cited by 63 · see citing works
Animal models and conserved processes
Greek, R., & Rice, M. J. (2012) · Theoretical Biology and Medical Modelling · doi:10.1186/1742-4682-9-40 · Cited by 44 · see citing works
Raphael, J., Selwyn, D. A., MOTTRAM, S. D., Langton, J. A., & O’Callaghan, C. (1996) · British Journal of Anaesthesia · doi:10.1093/bja/76.1.116 · Cited by 34 · see citing works
Physical Control Over Endocytosis
Baluška, F., & Wan, Y. (2012) · Springer eBooks · doi:10.1007/978-3-642-32463-5_6 · Cited by 31 · see citing works
Cervin, A., & Lindberg, S. I. (1998) · British Journal of Anaesthesia · doi:10.1093/bja/80.4.475 · Cited by 31 · see citing works
Hauser, M., & Eys, H. V. (1976) · Journal of Cell Science · doi:10.1242/jcs.20.3.589 · Cited by 29 · see citing works
Brosnan, R. J. (2013) · Veterinary Clinics of North America Equine Practice · doi:10.1016/j.cveq.2012.11.006 · Cited by 23 · see citing works
Membrane action of chloramphenicol measured by protozoan motility inhibition
Wu, C., Clift, P., Fry, C. H., & Henry, J. A. (1996) · Archives of Toxicology · doi:10.1007/s002040050349 · Cited by 22 · see citing works
Telser, A. (1977) · Experimental Cell Research · doi:10.1016/0014-4827(77)90406-2 · Cited by 17 · see citing works
The effect of lofepramine and other related agents on the motility of Tetrahymena pyriformis
Pf, D., Kelly, J. P., Leonard, B. E., & Henry, J. A. (2002) · Toxicology Letters · doi:10.1016/s0378-4274(01)00542-2 · Cited by 17 · see citing works
Bhashyam, A. R., Mogayzel, P. J., McGrath‐Morrow, S. A., Neptune, E., Malinina, A., Fox, J. M., & Laube, B. L. (2012) · PLoS ONE · doi:10.1371/journal.pone.0045312 · Cited by 15 · see citing works
Halothane and non-disjunction in drosophila
Clements, J., & Todd, N. K. (1981) · Mutation Research Letters · doi:10.1016/0165-7992(81)90036-1 · Cited by 15 · see citing works
EFFECT OF INHALATION ANAESTHETICS ON DIVISION OF BONE-MARROW CELLS IN VITRO
NUNN, J. F., Sturrock, J. E., & Howell, A. (1976) · British Journal of Anaesthesia · doi:10.1093/bja/48.2.75 · Cited by 15 · see citing works
Self-citationInhibition of sperm motility by the volatile anesthetic halothane
Hinkley, R. E. (1979) · Experimental Cell Research · doi:10.1016/0014-4827(79)90028-4 · Cited by 12 · see citing works
Membrane toxicity of opioids measured by protozoan motility
Wu, C., Fry, C. H., & Henry, J. A. (1997) · Toxicology · doi:10.1016/s0300-483x(96)03547-0 · Cited by 12 · see citing works
From individual to population: the minimum alveolar concentration curve
Dilger, J. P. (2006) · Current Opinion in Anaesthesiology · doi:10.1097/01.aco.0000236138.31099.fc · Cited by 11 · see citing works
Adamatzky, A., & Gandía, A. (2022) · Scientific Reports · doi:10.1038/s41598-021-04172-0 · Cited by 9 · see citing works
Baillie, A. J., AL-ASSADI, H. M. T., & Florence, A. T. (1989) · International Journal of Pharmaceutics · doi:10.1016/0378-5173(89)90318-9 · Cited by 9 · see citing works
Determination of membrane-stabilizing potency of drugs by video analysis of protozoan motility
Cassidy, S. L., Henry, J. A., & Roberts, A. M. (1989) · Toxicology in Vitro · doi:10.1016/0887-2333(89)90003-9 · Cited by 9 · see citing works
Effects of halothane on mucociliary activity in vivo
CERVIN, E., Lindberg, S. I., & Mercke, U. (1995) · Otolaryngology · doi:10.1016/s0194-5998(95)70181-8 · Cited by 8 · see citing works
Influence of anesthetic technique on early postoperative hypoxemia
Georgiou, L., Vourlioti, A. N., Kremastinou, F., Stefanou, P. S., Tsiotou, A. G., & Kokkinou, M. D. (1996) · Acta Anaesthesiologica Scandinavica · doi:10.1111/j.1399-6576.1996.tb04390.x · Cited by 8 · see citing works
The effect of halothane and pentobarbital sodium on brain ependymal cilia
O’Callaghan, C., & Sikand, K. S. (2012) · Cilia · doi:10.1186/2046-2530-1-12 · Cited by 7 · see citing works
Pressure enhancement of the depressant effect of halothane on cilial beat
Pope, W. D. B., Jones, A. J., Halsey, M. J., & NUNN, J. F. (1978) · Canadian Journal of Anesthesia/Journal canadien d anesthésie · doi:10.1007/bf03005656 · Cited by 7 · see citing works
Self-citationInhibition of Protozoan Motility by Local Anaesthetics
al-Saadi, D., Baillie, A. J., Farrell, W. E., Sneader, W. E., & Waton, N. G. (1981) · Journal of Pharmacy and Pharmacology · doi:10.1111/j.2042-7158.1981.tb11752.x · Cited by 6 · see citing works
Demography and movement patterns of a freshwater ciliate: The influence of oxygen availability
Brans, V., Manzi, F., Jacob, S., & Schtickzelle, N. (2024) · Ecology and Evolution · doi:10.1002/ece3.11291 · Cited by 5 · see citing works
Steffey, E. P., Brosnan, R. J., & Mama, K. R. (2024) · doi:10.1002/9781119830306.ch28 · Cited by 4 · see citing works
Yu, X., Qiu, Z., Nashrah, F., Garg, A., Chang, A., Schieber, A., Wu, S., Magavi, V., & Wiest, M. C. (2026) · Experimental Cell Research · doi:10.1016/j.yexcr.2026.114938 · Cited by 3 · see citing works
Jones, A. R., Wiklund, R. A., & NUNN, J. F. (1975) · Experimental Cell Research · doi:10.1016/0014-4827(75)90516-9 · Cited by 3 · see citing works
Self-citationFungi anaesthesia.Preprint
Adamatzky, A., & Gandía, A. (2021) · arXiv (Cornell University)
References and further reading
(1987) · Elsevier eBooks · doi:10.1016/b978-0-407-00342-2.50042-x
Adamatzky, A., & Gandía, A. (2023) · Emergence, complexity and computation · doi:10.1007/978-3-031-38336-6_5
On the functional significance of quantum effects at subneuronal scales
Roeloffs, J., & Tuszyński, J. A. (2026) · Biosystems · doi:10.1016/j.biosystems.2026.105905
- Authors
- Nunn, J. F., Sturrock, J. E., J-Wills, E., Richmond, J. E., & Mcpherson, C. K.
- Affiliations
- Division of Anaesthesia, Clinical Research Centre, Watford Road, Harrow, Middlesex HA1 3UJ, England
- Venue
- Journal of Cell Science
- Publisher
- The Company of Biologists
- Published
- 1974-08-01
- Volume
- 15
- Issue
- 3
- Pages
- 537-554
- ISSN
- 0021-9533 (ISSN-L)
- 1477-9137
- OpenAlex
- W2396835511
- Cited by
- 37
- References
- 28
- Citations by year
- 2026: 2 · 2024: 2 · 2023: 1 · 2022: 1 · 2021: 1 · 2019: 1 · 2015: 1 · 2013: 2 · 2012: 4
- FWCI
- 4.32
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Cell Science (publishedVersion)
- PubMed (publishedVersion)
- HighWire Press Open Archive (submittedVersion)
- Topics
- Health Sciences › Medicine › Anesthesiology and Pain Medicine › Anesthesia and Sedative Agents
- Life Sciences › Neuroscience › Developmental Neuroscience › Anesthesia and Neurotoxicity Research
- Health Sciences › Medicine › Surgery › Biomedical and Chemical Research
- Keywords
- inhalational anesthetics, Tetrahymena pyriformis, swimming speed, ciliary motility, halothane, cyclopropanes, anesthetic concentration, lipid solubility, oxygen consumption, deciliation, ciliary ultrastructure, basal bodies, mitochondrial swelling
- MeSH terms
- Anesthetics, Cell Movement, Chloroform, Cilia, Cyclopropanes, Depression, Chemical, Dose-Response Relationship, Drug, Ethyl Ethers, Halothane, Methoxyflurane, Microscopy, Electron, Mitochondrial Swelling, Oxygen Consumption, Solubility, Tetrahymena pyriformis, Trichloroethylene
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