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Oxygen limitation and thermal tolerance in two terrestrial arthropod species
Open accessAbstract
SUMMARY: Recent studies of marine invertebrates and fish have suggested that lower and upper critical temperatures (CTmin and CTmax) are coupled by a common mechanism: oxygen and capacity limitation of thermal tolerance (OCLT). Using thermolimit respirometry, we tested the predictions of this theory for terrestrial arthropods by measuring maxima and minima for both critical temperatures and metabolic rate in two arthropods, the isopod Porcellio scaber and the beetle Tenebrio molitor, at 40%, 21%, 10% and 2.5% ambient O2. Critical temperatures were identified as particular points on both activity and traces in four ways. In the first two instances, we identified the inflection points in regressions of absolute difference sum (ADS) residuals calculated for activity (aADS) and (VI), respectively. In the third, we visually identified the lowest point before the post-mortal peak in CO2 release (PMV). Finally, we pinpointed the sudden drop in at death, where fell outside the 95% confidence intervals of the 5 min period immediately preceding the drop-off (CI). Minimum and maximum metabolic rates were determined using CO2 traces, and the temperatures corresponding to these identified as TMetMin and TMetMax. For both species, ambient oxygen concentration did not influence CTmin, minimum metabolic rate, or TMetMin. By contrast, severe hypoxia (2.5% O2) caused a 6.9°C decline in activity-based CTmax for T. molitor and a 10.6°C decline for P. scaber, relative to normoxia (21% O2). The magnitude of this decrease differed between methods used to estimated critical thermal limits, highlighting the need for a standard method to determine these endpoints during thermolimit respirometry. Maximum metabolic rate also declined with decreasing ambient oxygen in both species. The combination of increasing metabolic rate and oxygen limitation affected upper thermal limits in these arthropods only in severe hypoxia (2.5% O2). In both species, CTmin and CTmax responded differently to oxygen limitation, suggesting that this is not a common mechanism coupling upper and lower limits in terrestrial arthropods.
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Metadata
- Authors
- Stevens, M. M., Jackson, S., Bester, S. A., Terblanche, J. S., & Chown, S. L.
- Affiliations
- Stellenbosch University (ZA)
- Corresponding author
- Jackson, Sue
- Venue
- Journal of Experimental Biology
- Publisher
- The Company of Biologists
- Published
- 2010-07-01
- Volume
- 213
- Issue
- 13
- Pages
- 2209-2218
- ISSN
- 0022-0949 (ISSN-L)
- 1477-9145
- OpenAlex
- W2157683714
- Cited by
- 115
- References
- 2
- Citations by year
- 2026: 4 · 2025: 6 · 2024: 3 · 2023: 5 · 2022: 3 · 2021: 9 · 2020: 7 · 2019: 7 · 2018: 8 · 2017: 6 · 2016: 9 · 2015: 12 · 2014: 10 · 2013: 7 · 2012: 12
- FWCI
- 7.50
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Experimental Biology (publishedVersion)
- PubMed (publishedVersion)
- SUNScholar (Stellenbosch University) (submittedVersion)
- Funders
- National Research Foundation
- Topics
- Physical Sciences › Environmental Science › Ecology › Physiological and biochemical adaptations
- Physical Sciences › Environmental Science › Ecology › Crustacean biology and ecology
- Life Sciences › Agricultural and Biological Sciences › Ecology, Evolution, Behavior and Systematics › Tardigrade Biology and Ecology
- Keywords
- oxygen limitation, heat tolerance, critical thermal limits, hypoxia, Porcellio scaber, Tenebrio molitor, terrestrial arthropods, metabolic rate, upper critical temperature, lower critical temperature
- MeSH terms
- Animals, Oxygen, Temperature, Tenebrio, Isopoda
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