Caffeic acid protects hydrogen peroxide induced cell damage in wi-38 human lung fibroblast cells
Open accessAbstract
Cytoprotective effect of caffeic acid (3,4-dihydroxy cinnamic acid) on human lung fibroblast (WI-38) cells against hydrogen peroxide induced damage was investigated. Caffeic acid was found to scavenge intracellular reactive oxygen species, and 1,1-diphenyl-2-picrylhydrazyl radical, and thus prevented lipid peroxidation. The caffeic acid protected cell damage of WI-38 cells exposed to hydrogen peroxide (H(2)O(2)), via the activation of extracellular signal regulated kinase protein. Caffeic acid increased the activity of catalase and its protein expression. Hence, from the present study, it is suggestive that caffeic acid protects WI-38 cells against H2O2 damage by enhancing the cellular antioxidant activity.
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Treatment of WI-38 lung fibroblast cells with caffeic acid (0.5, 5 and 5 μM) reduced the fluorescence intensity by H2O2 treatment, reflecting a reduction of ROS generation.
“In a previous study it was demonstrated that treatment of WI-38 lung fibroblast cells with caffeic acid (0.5, 5 and 5 µM) reduced the fluorescence intensity by H 2 O 2 treatment, reflecting a reduction of ROS generation (Kang et al. 2006).”
From Evaluation of chemical constituents and antioxidant activity of coconut water (Cocus nucifera L.) and caffeic acid in cell culture (2013) · cites this paper as Kang et al. 2006
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SANTOS, J. L. A., BISPO, V. S., B.C. FILHO, A., PINTO, I. F. D., DANTAS, L. S., VASCONCELOS, D. F., ABREU, F. F., MELO, D. A., MATOS, I. A., FREITAS, F. P., et al. (2013) · Anais da Academia Brasileira de Ciências · doi:10.1590/0001-37652013105312 · Cited by 61 · see citing works
- Authors
- Kang, K. A., Lee, K. H., Zhang, R., Piao, M., Chae, S., Kim, K. N., Jeon, Y. J., Park, D. B., You, H. J., Kim, J. S., & Hyun, J. W.
- Affiliations
- Jeju National University (KR)
- Department of Biochemistry, College of Medicine and Applied Radiological Science Research Institute, Cheju National University
- Department of Food Science and Technology, Cheju National University
- Chosun University (KR)
- Korea Institute of Oriental Medicine (KR)
- Venue
- Biological and Pharmaceutical Bulletin
- Publisher
- Pharmaceutical Society of Japan
- Published
- 2006
- Volume
- 29
- Issue
- 9
- Pages
- 1820-1824
- ISSN
- 0918-6158 (ISSN-L)
- 1347-5215
- OpenAlex
- W2007013247
- Cited by
- 32
- References
- 37
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 1 · 2023: 3 · 2022: 2 · 2021: 1 · 2019: 1 · 2018: 2 · 2017: 4 · 2016: 2 · 2015: 2 · 2014: 1 · 2013: 1 · 2012: 2
- FWCI
- 1.35
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Biological and Pharmaceutical Bulletin (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
- Physical Sciences › Chemistry › Organic Chemistry › Free Radicals and Antioxidants
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Keywords
- caffeic acid, hydrogen peroxide, oxidative stress, cytoprotection, reactive oxygen species scavenging, lipid peroxidation, DPPH radical, ERK activation, catalase, antioxidant activity
- MeSH terms
- Caffeic Acids, Catalase, Cells, Cultured, Fibroblasts, Humans, Hydrogen Peroxide, Lung, Lipid Peroxidation, Free Radical Scavengers, Cytoprotection, Extracellular Signal-Regulated MAP Kinases
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