Analysis of genetic stability through intersimple sequence repeats molecular markers in micropropagated plantlets of Anoectochilus formosanus Hayata, a medicinal plant
Open accessAbstract
Anoectochilus formosanus HAYATA, commonly known as "Jewel Orchids," which has been used as Chinese folk medicines, is being subjected to a huge crisis, whose wild resources have gradually become more and more scarce. Hence, micropropagation protocol by axillary branching established for A. formosanus was employed for large-scale commercial production. In this study, due to the somaclonal variation and on the basis of some virtue of intersimple sequence repeats (ISSR) analysis, we firstly utilized the ISSR primers to investigate the genetic stability of A. formosanus propagated in vitro for a period of more than 5 years. Among the total 100 bands amplified by 17 ISSR markers, 77 bands were distributed in size from 500 bp to 1.5 kbp, while only 5 bands were beyond 1.8 kbp in size. Meanwhile, according to the cluster analysis, genetic similarity was more than 94% and the polymorphism rate was only 2.76% among the total 1810 scorable bands. All results demonstrate A. formosanus, multiplied by axillary branching, maintained high genetic fidelity even after a period of more than 5 years under in vitro propagation with only a low risk of genetic instability. The results from this study provide an important basis for giving evidence of genetic stability of A. formosanus before micropropagation for large-scale commercial production.
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ISSR markers, applied comparatively between plantlets and mother plant, generally revealed the clonal nature of analyzed regenerants, highlighting genetic fidelity of in vitro propagation, even after several years.
“However, the ISSR markers, applied comparatively between the plantlets and the mother plant revealed, in general, the clonal nature of the analyzed regenerants, highlighting the genetic fidelity of in vitro propagation, even after several years [70,71,72].”
From Somaclonal Variation—Advantage or Disadvantage in Micropropagation of the Medicinal Plants (2023) · cites this paper as [71]
The Discussion (page not provided) concludes that ISSR results showed high genetic fidelity in A. formosanus after more than five years of in vitro propagation. But Materials and Methods (page not provided) identifies the controls as two-year micropropagated plantlets from the same mother seedling, not the mother plant itself; the claimed direct comparison with the mother plant is therefore unsupported. (LLM, conf=0.88)
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Cites
Venkatachalam, L., Sreedhar, R. V., & Bhagyalakshmi, N. (2007) · Plant Growth Regulation · doi:10.1007/s10725-006-9154-y · Cited by 93 · see citing works
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Research works printed but not linked: 5
- Editorial Committee of Flora of China & Chinese Academy of Sci- ences, "Flora Reipublicae Popularis Sinicae," Science Press, Beijing, 2000.
- Institute of Research on Traditional Chinese Medicine of Fujian Province, "Record of Fujian Materia Medica," Fujian Science and Technology Press, Fuzhou, 1982.
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Somaclonal Variation—Advantage or Disadvantage in Micropropagation of the Medicinal Plants
Duta-Cornescu, G., Constantin, N., Pojoga, D.-M., Nicuta, D., & Simon-Gruita, A. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms24010838 · Cited by 108 · see citing works
“76% polymorphism—low risk of genetic instability, high genetic fidelity [71]”
BackgroundTikendra, L., Potshangbam, A. M., Dey, A., Devi, T. R., Sahoo, M. R., & Nongdam, P. (2021) · Physiology and Molecular Biology of Plants · doi:10.1007/s12298-021-00939-x · Cited by 83 · see citing works
Evaluation of Genetic Stability of In Vitro Raised Orchids Using Molecular-Based Markers
Mohapatra, P., Ray, A., & Jena, S. (2022) · Commercial Scale Tissue Culture for Horticulture and Plantation Crops · doi:10.1007/978-981-19-0055-6_13 · Cited by 5 · see citing works
- Authors
- Zhang, F., Lv, Y., Dong, H., & Guo, S.
- Affiliations
- Chinese Academy of Medical Sciences & Peking Union Medical College (CN)
- Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College
- Venue
- Biological and Pharmaceutical Bulletin
- Publisher
- Pharmaceutical Society of Japan
- Published
- 2010
- Volume
- 33
- Issue
- 3
- Pages
- 384-388
- ISSN
- 0918-6158 (ISSN-L)
- 1347-5215
- OpenAlex
- W2056961063
- Cited by
- 36
- References
- 1
- Citations by year
- 2026: 1 · 2023: 5 · 2022: 3 · 2021: 5 · 2019: 2 · 2018: 2 · 2017: 3 · 2016: 2 · 2015: 2 · 2014: 7 · 2013: 2 · 2012: 1
- FWCI
- 0.52
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Biological and Pharmaceutical Bulletin (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National High-tech Research and Development Program
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic diversity and population structure
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic Mapping and Diversity in Plants and Animals
- Keywords
- Anoectochilus formosanus, genetic stability, somaclonal variation, ISSR markers, micropropagation, axillary branching, genetic fidelity, medicinal plants, genetic polymorphism
- MeSH terms
- Agriculture, Plants, Medicinal, Polymorphism, Genetic, Cluster Analysis, Plant Shoots, DNA, Plant, Genome, Plant, Tandem Repeat Sequences, Orchidaceae, Genomic Instability
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