RAPD, ISSR, and SCoT markers based genetic stability assessment of micropropagated Dendrobium fimbriatum Lindl. var. oculatum Hk. f.—An important endangered orchid
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Dendrobium fimbriatum is an ornamental and medicinal orchid listed in the Red data book of IUCN. Phytohormones' effect on the in vitro regeneration of the orchid was studied using Mitra medium supplemented with different growth regulators. KN produced effective shoot formation when present alone or in combination with IBA or NAA. The shooting was gradually increased when KN concentration was increased from 0.8 to 4.8 mg L-1, but the opposite response was observed with BAP at higher concentration (4.8 mg L-1). IBA either in combination with BAP or KN promoted effective root development and multiplication. Micropropagated orchids grown in the basal medium devoid of any phytohormone showed 100% monomorphism, while low genetic polymorphism of 1.52% (RAPD-Random Amplification of Polymorphic DNA), 1.19% (ISSR-Inter Simple Sequence Repeat) and 3.97% (SCoT-Start Codon Targeted) was exhibited among the regenerants propagated in the hormone enriched medium. UPGMA (Unweighted pair group method using arithmetic averages) dendrograms showed the grouping of mother plant (MP) with the in vitro regenerants. The principal coordinate analysis (PCoA) further confirmed the clustering patterns as determined by the cluster analysis. The study reported for the first time the successful in vitro propagation of Dendrobium fimbriatum and their genetic stability assessment using molecular markers.
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In studies using a combination of two or three types of markers (SCoT, ISSR, RAPD), research revealed genetic fidelity of regenerated plantlets, even for those that had passed through the callus stage.
“There are a number of studies in which the somaclonal variation analysis is carried out using a combination of two or three types of markers (Table 2), such as SCot, ISSR, and RAPD, and, in all cases, the research revealed the genetic fidelity of the regenerated plantlets (even for those that had passed through the callus stage) [73,74,75,76,77,78].”
From Somaclonal Variation—Advantage or Disadvantage in Micropropagation of the Medicinal Plants (2023) · cites this paper as [76]
The Molecular marker polymorphism section reports that combined RAPD, ISSR and SCoT analysis found high genetic stability in regenerated orchids. It also reports 2.70% polymorphism, so the plantlets were not all genetically identical to the mother plant. The Micropropagation section describes regeneration through protocorms, not a callus stage; this paper does not support the claim about plantlets that passed through callus. (LLM, conf=0.87)
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- Authors
- Tikendra, L., Potshangbam, A. M., Dey, A., Devi, T. R., Sahoo, M. R., & Nongdam, P.
- Affiliations
- Manipur University (IN)
- Presidency University (IN)
- Indian Council of Agricultural Research (IN)
- Corresponding author
- Nongdam, Potshangbam
- ORCID
- Venue
- Physiology and Molecular Biology of Plants
- Published
- 2021-02
- Volume
- 27
- Issue
- 2
- Pages
- 341-357
- ISSN
- 0971-5894 (ISSN-L)
- 0974-0430
- PMC
- PMC7907394
- OpenAlex
- W3130899892
- Cited by
- 83
- References
- 78
- Citations by year
- 2026: 12 · 2025: 19 · 2024: 16 · 2023: 19 · 2022: 15 · 2021: 2
- FWCI
- 4.08
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Physiology and Molecular Biology of Plants (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Department of Science and Technology, Ministry of Science and Technology
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic diversity and population structure
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Flowering Plant Growth and Cultivation
- Keywords
- orchid micropropagation, genetic stability, in vitro regeneration, kinetin, indole-3-butyric acid, naphthaleneacetic acid, benzyladenine, shoot multiplication, root development, RAPD markers, ISSR markers, SCoT markers, genetic polymorphism, endangered orchids, medicinal orchids
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