Development of a Screening System for Targeting Carriers Using Peptide-Modified Liposomes and Tissue Sections
Open accessAbstract
Liposomes have been used as targeting carriers for drug delivery systems (DDSs), and the carriers are able to be modified with targeting ligands, such as antibodies and peptides. To evaluate the targetability of DDS carriers modified with a targeting ligand, culture cells expressing the targeting molecules as well as small animals are used. Furthermore, in vitro and in vivo screening analyses must be repeatedly performed. Therefore, it is important to establish an easy and high-precision screening system for targeting carriers. With this aim, we focused that whether this ex vivo system could easily support assessment of interaction between targeting ligand and its receptor under physiological environment and further screen the DDS carrier-modified with targeting moiety. We examined targeting ability via in vitro, ex vivo, and in vivo analyses using integrin αvβ3-targeting C16Y-L. For the in vitro analysis, the cellular uptake of C16Y-L was higher than that of control liposomes in colon26 cells. For the ex vivo analysis, we performed an immunohistochemical analysis using colon26 tumor sections. C16Y-L was specifically attached to the tumor sections, as found in the in vitro analysis. Moreover, to evaluate the ex vivo–in vivo correlation, we examined the intratumoral localization of C16Y-L. This result showed that C16Y-L was accumulated not only in the tumor tissue but also in the tumor vasculature after the intravenous injection of C16Y-L, suggesting that the ex vivo peptide-modified liposomal analysis was correlated with the in vivo analysis. Thus, the ex vivo peptide-modified liposomal analysis may be an easy and rapid screening system with high-precision and for consideration in in vivo conditions.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
C16Y-L accumulates in tumor tissues and tumor blood vessels in vivo.
“In vivo studies have found that C16Y-L not only accumulates in tumor tissues, but also in tumor blood vessels.”
From Smart nanoparticles for cancer therapy (2023) · cites this paper as [220]
C16Y-L has higher cellular uptake in colon 26 cells than liposomes and specifically attaches to tumor slices.
“Its cellular uptake in colon 26 cells is higher than that of liposomes, and it can specifically attach to tumor slices.”
From Smart nanoparticles for cancer therapy (2023) · cites this paper as [220]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Amphipathic polyethyleneglycols effectively prolong the circulation time of liposomes
Klibanov, A. L., Maruyama, K., Torchilin, V. P., & Huang, L. (1990) · FEBS Letters · doi:10.1016/0014-5793(90)81016-h · Cited by 2,030 · see citing works
Allen, T. M., Hansen, C., Martin, F., Redemann, C. T., & Yau‐Young, A. O. (1991) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/0005-2736(91)90246-5 · Cited by 1,392 · see citing works
Gabizon, A. (2004) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2004.01.011 · Cited by 464 · see citing works
Lukyanov, A. N., ElBayoumi, T. A., Chakilam, A. R., & Torchilin, V. P. (2004) · Journal of Controlled Release · doi:10.1016/j.jconrel.2004.08.007 · Cited by 294 · see citing works
Effective anti-tumor activity of oxaliplatin encapsulated in transferrin–PEG-liposome
Suzuki, R., 知子, 滝., Kuwata, Y., Mutoh, M., Ishiguro, N., Utoguchi, N., Shinohara, A., Eriguchi, M., Yanagië, H., & Maruyama, K. (2007) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2007.06.010 · Cited by 250 · see citing works
pH‐sensitive liposomes mediate cytoplasmic delivery of encapsulated macromolecules
Straubinger, R. M., Düzgüneş, N., & Papahadjopoulos, D. (1985) · FEBS Letters · doi:10.1016/0014-5793(85)80210-6 · Cited by 240 · see citing works
Maruyama, K., Yuda, T., Okamoto, A., Kojima, S., Suginaka, A., & Iwatsuru, M. (1992) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(92)90255-t · Cited by 230 · see citing works
Maruyama, K., Ishida, O., Kasaoka, S., et al. (2004) · Journal of Controlled Release · doi:10.1016/j.jconrel.2004.04.018 · Cited by 207 · see citing works
Xiong, X.-B., Huang, Y., Lü, W.-L., Zhang, X., Zhang, H., Nagai, T., & Zhang, Q. (2005) · Journal of Controlled Release · doi:10.1016/j.jconrel.2005.03.030 · Cited by 205 · see citing works
Tumor targeting of doxorubicin by anti-MT1-MMP antibody-modified PEG liposomes
Hatakeyama, H., Akita, H., Ishida, E., Hashimoto, K., Kobayashi, H., Aoki, T., Yasuda, J., Obata, K., Kikuchi, H., Ishida, T., et al. (2007) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2007.04.037 · Cited by 165 · see citing works
Synthesis of Cetuximab-Immunoliposomes via a Cholesterol-Based Membrane Anchor for Targeting of EGFR
Pan, X., Wu, G. J., Yang, W., et al. (2006) · Bioconjugate Chemistry · doi:10.1021/bc060174r · Cited by 134 · see citing works
Biodistribution characteristics of mannosylated, fucosylated, and galactosylated liposomes in mice
Kawakami, S., Wong, J., Sato, A., Hattori, Y., Yamashita, F., & Hashida, M. (2000) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/s0304-4165(00)00163-x · Cited by 124 · see citing works
An angiogenic laminin site and its antagonist bind through the αvβ3 and α5β1 integrins
Ponce, M. L., Nomizu, M., & Kleinman, H. K. (2001) · The FASEB Journal · doi:10.1096/fj.00-0736com · Cited by 88 · see citing works
Self-citationNegishi, Y., Omata, D., Iijima, H., Takabayashi, Y., Suzuki, K., Endo‐Takahashi, Y., Suzuki, R., Maruyama, K., Nomizu, M., & Aramaki, Y. (2009) · Molecular Pharmaceutics · doi:10.1021/mp900214s · Cited by 84 · see citing works
Self-citationHarata, M. (2004) · Blood · doi:10.1182/blood-2004-02-0588 · Cited by 75 · see citing works
AG73-modified Bubble liposomes for targeted ultrasound imaging of tumor neovasculature
Negishi, Y., Hamano, N., Tsunoda, Y., et al. (2012) · Biomaterials · doi:10.1016/j.biomaterials.2012.09.056 · Cited by 72 · see citing works
Self-citationPonce, M. L., Hibino, S., Lebioda, A. M., et al. (2003) · PubMed · Cited by 58 · see citing works
Temming, K., Meyer, D. L., Zabinski, R. F., et al. (2006) · Bioconjugate Chemistry · doi:10.1021/bc060087z · Cited by 52 · see citing works
Low pH induced membrane fusion of lipid vesicles containing proton-sensitive polymer
Oku, N., Shibamoto, S., Ito, F., et al. (1987) · Biochemistry · doi:10.1021/bi00399a019 · Cited by 41 · see citing works
Séguin, J., Nicolazzi, C., Mignet, N., et al. (2012) · Tumor Biology · doi:10.1007/s13277-012-0428-x · Cited by 39 · see citing works
Hamano, N., Negishi, Y., Fujisawa, A., et al. (2012) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2012.02.006 · Cited by 21 · see citing works
Self-citation
Flagged first, then by citations
Research works printed but not linked: 3
- Maruyama K, Yuda T, Okamoto A, Kojima S, Suginaka A, Iwa- tsuru M. Prolonged circulation time in vivo of large unilamellar liposomes composed of distearoyl phosphatidylcholine and choles- terol containing amphipathic poly(ethylene glycol). Biochim. Bio- phys. Acta, 1128, 44-49 (1992). · DOI
- Ponce ML, Nomizu M, Kleinman HK. An angiogenic laminin site and its antagonist bind through the alpha(v)beta3 and alpha5beta1 integrins. FASEB J., 15, 1389-1397 (2001). · DOI
- Temming K, Meyer DL, Zabinski R, Dijkers EC, Poelstra K, Molema G, Kok RJ. Evaluation of RGD-targeted albumin carriers for specific delivery of auristatin E to tumor blood vessels. Biocon- jug. Chem., 17, 1385-1394 (2006). · DOI
Cited by
Sorted by citations
Smart nanoparticles for cancer therapy
Sun, L., Liu, H., Ye, Y., Lei, Y., Islam, R., Tan, S., Tong, R., Miao, Y.-B., & Cai, L. (2023) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-023-01642-x · Cited by 1,073 · see citing works
- Authors
- Negishi, Y., Hamano, N., Sato, H., Katagiri, F., Takatori, K., Endo-Takahashi, Y., Kikkawa, Y., & Nomizu, M.
- Affiliations
- Tokyo University of Pharmacy and Life Sciences (JP)
- Department of Drug Delivery and Molecular Biopharmaceutics, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences
- Venue
- Biological and Pharmaceutical Bulletin
- Publisher
- Pharmaceutical Society of Japan
- Published
- 2018-07-01
- Volume
- 41
- Issue
- 7
- Pages
- 1107-1111
- ISSN
- 0918-6158 (ISSN-L)
- 1347-5215
- OpenAlex
- W2810546913
- Cited by
- 8
- References
- 21
- Citations by year
- 2026: 1 · 2025: 3 · 2023: 1 · 2020: 1 · 2019: 2
- FWCI
- 0.24
- Type
- journal article
- Language
- en
- Open access
- diamond
- doi.org
- Locations
- Biological and Pharmaceutical Bulletin (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministry of Education, Culture, Sports, Science and Technology
- Japan Society for the Promotion of Science (17K07180)
- Topics
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advanced Drug Delivery Systems
- Keywords
- integrin αvβ3, tumor vasculature targeting, drug delivery systems, ligand-receptor interactions
- MeSH terms
- Animals, Antineoplastic Agents, Chemistry, Pharmaceutical, Humans, Ligands, Liposomes, Male, Mice, Inbred BALB C, Mice, Inbred C57BL, Neoplasms, Oligopeptides, Drug Delivery Systems, Xenograft Model Antitumor Assays, Integrin alphaVbeta3, Cell Line, Tumor, Mice, Nanoparticles, High-Throughput Screening Assays, Human Umbilical Vein Endothelial Cells
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
