Tumor Necrosis Factor-α Mediates the Blood–Brain Barrier Dysfunction Induced by Activated Microglia in Mouse Brain Microvascular Endothelial Cells
Open accessAbstract
The present study was designed to elucidate the involvement of tumor necrosis factor-alpha (TNF-alpha) release from activated microglia in the induction of blood-brain barrier (BBB) dysfunction in an in vitro co-culture system with mouse brain capillary endothelial cells (MBEC4) and microglia. Lipopolysaccharide (LPS)-activated microglia increased the permeability of MBEC4 cells to sodium-fluorescein, and this hyper-permeability was blocked by a neutralizing antibody against TNF-alpha. LPS stimulated microglia to facilitate TNF-alpha release. These findings suggested that TNF-alpha released from activated microglia is attributable to BBB dysfunction.
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TNF-α released from activated microglia contributes to BBB dysfunction.
“indicating that TNF-α contributes to BBB dysfunction (Nishioku et al., 2010).”
From The impact of microglial activation on blood-brain barrier in brain diseases (2014) · cites this paper as Nishioku et al., 2010
LPS induces production of TNF-α in the brain by microglial cells, which promotes BBB dysfunction and also causes neuronal cell death by phagocytosis.
“On the other hand, it has been shown that LPS induces the production of TNF-α in the brain by microglial cells, which promotes BBB dysfunction and also causes neuronal cell death by phagocytosis (Tanaka et al., 2006; Nishioku et al., 2010; Smith et al., 2012; Neniskyte et al., 2014).”
From The impact of microglial activation on blood-brain barrier in brain diseases (2014) · cites this paper as Nishioku et al., 2010
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- Authors
- Nishioku, T., Matsumoto, J., Dohgu, S., Sumi, N., Miyao, K., Takata, F., Shuto, H., Yamauchi, A., & Kataoka, Y.
- Affiliations
- Fukuoka University (JP)
- Venue
- Journal of Pharmacological Sciences
- Publisher
- Japanese Pharmacological Society
- Published
- 2010
- Volume
- 112
- Issue
- 2
- Pages
- 251-254
- ISSN
- 1347-8613 (ISSN-L)
- 1347-8648
- OpenAlex
- W2047074480
- Cited by
- 168
- References
- 16
- Citations by year
- 2026: 5 · 2025: 9 · 2024: 13 · 2023: 14 · 2022: 14 · 2021: 15 · 2020: 17 · 2019: 7 · 2018: 12 · 2017: 11 · 2016: 8 · 2015: 10 · 2014: 12 · 2013: 4 · 2012: 7
- FWCI
- 3.86
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Pharmacological Sciences (publishedVersion)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- University of Tokyo
- Japan Society for the Promotion of Science (21790526, 21790257, 21790102, 20800066, 21790255, 17390159)
- Division of Molecular and Cellular Biosciences
- Topics
- Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Health Sciences › Medicine › Neurology › Traumatic Brain Injury and Neurovascular Disturbances
- Keywords
- TNF-α, blood-brain barrier dysfunction, microglial activation, brain microvascular endothelial cells, endothelial permeability, lipopolysaccharide, anti-TNF-alpha antibody, neuroinflammation
- MeSH terms
- Animals, Blood-Brain Barrier, Brain, Capillaries, Cell Membrane Permeability, Endothelium, Vascular, Lipopolysaccharides, Mice, Inbred BALB C, Tumor Necrosis Factor-alpha, Microglia, Coculture Techniques, Fluorescein, Mice
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