Effects of streptozotocin-induced diabetes in domestic pigs with focus on the amino acid metabolism
Abstract
Streptozotocin (STZ) given intravenously destroys pancreatic beta cells and is widely used in animal models to mimic type 1 diabetes. The effects of STZ on the clinical state of health and metabolism were studied in six high health certified domestic pigs weighing 19+/-1.3 kg at the start of the experiment. A single STZ dose of 150 mg/kg of body weight successfully induced hyperglycaemia and alterations in amino acid metabolism. Within 9 h after STZ administration, the blood glucose values fell from 5.4-7.5 mmol/L to 0.8-2.2 mmol/L. Hypoglycaemia was treated with 0.5 g glucose/kg body weight. In all pigs, hyperglycaemia was produced 24 h after STZ treatment, and 3 days after STZ injection, the glucose concentration was >25 mmol/L. Mean C-peptide concentration was 0.25+/-0.16 microg/L since 2 days after STZ injection until the end of the study. The serum concentration of the branched-chain amino acids (BCAA) increased four-fold, and alanine and taurine decreased by approximately 70% and 50%, respectively, after STZ treatment. All but one pig remained brisk and the physical examination was normal except for a retarded growth rate and a reduction of the skeletal muscle. At the end of the study, the pigs were moderately emaciated. Postmortem examination confirmed muscle wasting and a reduction of abdominal and subcutaneous fat. In conclusion, STZ-induced diabetes in pigs fulfils the requirements for a good animal model for type 1 diabetes with respect to clinical signs of the dis…
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Five preclinical studies were conducted using nine healthy swine without diabetes and three with streptozotocin-induced diabetes.
“Five preclinical studies were conducted (Table 1), using nine healthy swine without diabetes and three with streptozotocin-induced diabetes.22”
From Development of a Real-time Force-based Algorithm for Infusion Failure Detection (2024) · cites this paper as [22]
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- Authors
- Jensen-Waern, M., Andersson, M., Kruse, R., Nilsson, B., Larsson, R., Korsgren, O., & Essén-Gustavsson, B.
- Affiliations
- Department of Clinical Sciences, Section for Comparative Physiology and Medicine, SLU, PO Box 7054, 750 07 Uppsala, Sweden
- Uppsala University (SE)
- Venue
- Laboratory Animals
- Publisher
- SAGE Publications
- Published
- 2009-07
- Volume
- 43
- Issue
- 3
- Pages
- 249-254
- ISSN
- 0023-6772 (ISSN-L)
- 1758-1117
- OpenAlex
- W2145962263
- Cited by
- 39
- References
- 32
- Citations by year
- 2025: 3 · 2024: 4 · 2023: 4 · 2022: 4 · 2021: 1 · 2020: 4 · 2019: 2 · 2018: 4 · 2017: 1 · 2016: 4 · 2015: 1 · 2014: 2 · 2013: 1 · 2012: 2
- FWCI
- 0.97
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Laboratory Animals (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diabetes Management and Research
- Health Sciences › Medicine › Surgery › Pancreatic function and diabetes
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Diabetes and associated disorders
- Keywords
- streptozotocin, type 1 diabetes, animal models of diabetes, domestic pigs, amino acid metabolism, branched-chain amino acids, hyperglycemia, hypoglycemia, C-peptide, pancreatic beta-cell destruction, muscle atrophy, fat loss, alanine, taurine
- MeSH terms
- Alanine, Amino Acids, Amino Acids, Branched-Chain, Animals, Blood Glucose, Body Weight, C-Peptide, Diabetes Mellitus, Experimental, Diabetes Mellitus, Type 1, Hyperglycemia, Insulin, Swine, Taurine, Muscle, Skeletal
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