New microRNAs from mouse and human
Abstract
MicroRNAs (miRNAs) represent a new class of noncoding RNAs encoded in the genomes of plants, invertebrates, and vertebrates. MicroRNAs regulate translation and stability of target mRNAs based on (partial) sequence complementarity. Although the number of newly identified miRNAs is still increasing, target mRNAs of animal miRNAs remain to be identified. Here we describe 31 novel miRNAs that were identified by cloning from mouse tissues and the human Saos-2 cell line. Fifty-three percent of all known mouse and human miRNAs have homologs in Fugu rubripes (pufferfish) or Danio rerio (zebrafish), of which almost half also have a homolog in Caenorhabditis elegans or Drosophila melanogaster. Because of the recurring identification of already known miRNAs and the unavoidable background of ribosomal RNA breakdown products, it is believed that not many more miRNAs may be identified by cloning. A comprehensive collection of miRNAs is important for assisting bioinformatics target mRNA identification and comprehensive genome annotation.
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This study confirms the expression of previously cloned miRNAs.
“This study confirms the expression of previously cloned miRNAs [1-3,9,10]”
Our characterization of organ expression of miRNAs correlates with the organ(s) from which these miRNAs were originally cloned.
“Our characterization of organ expression of miRNAs correlates with the organ(s) from which these miRNAS were originally cloned [2,3].”
We observed some discrepancies between our expression profiling by northern analysis and expression profiling by cloning, particularly for certain less-abundant miRNAs represented in size-selected RNA libraries by three or fewer copies.
“we observed some discrepancies between our expression profiling by northern analysis and expression profiling by cloning [2,3]. This is particularly so for certain less-abundant miRNAs - those that were represented in size-selected RNA libraries by three or fewer copies.”
The panel of organs tested did not include all the organs and tissues that miRNAs were cloned from, and thus some organ-specific or organ-enriched miRNAs may be expressed in additional organs or tissues.
“The panel of organs that we tested in our study did not include all the organs and tissues that miRNAs were cloned from [1-3,9,10], and thus some organ-specific or organ-enriched miRNAs may be expressed in additional organs or tissues.”
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- Authors
- LAGOS-QUINTANA, M., RAUHUT, R., MEYER, J., BORKHARDT, A., & TUSCHL, T.
- Venue
- RNA
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2003-02
- Volume
- 9
- Issue
- 2
- Pages
- 175-179
- ISSN
- 1355-8382
- 1469-9001
- Cited by
- 714
- References
- 2
- Language
- en
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