Probing microRNAs with microarrays: tissue specificity and functional inference
Abstract
MicroRNAs (miRNAs) are short, stable, noncoding RNAs involved in post-transcriptional gene silencing via hybridization to mRNA. Few have been thoroughly characterized in any species. Here, we describe a method to detect miRNAs using micro-arrays, in which the miRNAs are directly hybridized to the array. We used this method to analyze miRNA expression across 17 mouse organs and tissues. More than half of the 78 miRNAs detected were expressed in specific adult tissues, suggesting that miRNAs have widespread regulatory roles in adults. By comparing miRNA levels to mRNA levels determined in a parallel microarray analysis of the same tissues, we found that the expression of target mRNAs predicted on the basis of sequence complementarity is unrelated to the tissues in which the corresponding miRNA is expressed.
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A subset of miR-15/107 miRNA genes has been detected in mammalian tissues including heart, skeletal muscle, brain, lung, liver, kidney, spleen and placenta.
“A subset of these miRNA genes has been detected in mammalian tissues including heart, skeletal muscle, brain, lung, liver, kidney, spleen and placenta [25–31].”
From Expression of miR-15/107 Family MicroRNAs in Human Tissues and Cultured Rat Brain Cells (2014) · cites this paper as [29]
The Figure 4 discussion reports detection of miRNAs across 17 mouse tissues, and the Figure 2 discussion identifies miR-15b among miRNAs found in embryonic stem cells. Our copy does not establish that members of the miR-15/107 group were detected across the listed organs, particularly kidney and placenta. (LLM, conf=0.78)
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- Authors
- BABAK, T., ZHANG, W., MORRIS, Q., BLENCOWE, B. J., & HUGHES, T. R.
- Venue
- RNA
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2004-10-20
- Volume
- 10
- Issue
- 11
- Pages
- 1813-1819
- ISSN
- 1355-8382
- 1469-9001
- Cited by
- 327
- References
- 4
- Language
- en
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