The DOI matches its Crossref record.
Improved Gateway Binary Vectors: High-Performance Vectors for Creation of Fusion Constructs in Transgenic Analysis of Plants
Open accessAbstract
We made a series of improved Gateway binary vectors (pGWBs) for plant transformation. Fifteen different reporters and tags, sGFP, GUS, LUC, EYFP, ECFP, G3GFP, mRFP, 6xHis, FLAG, 3xHA, 4xMyc, 10xMyc, GST, T7, and TAP, were employed. Some vectors carry the 2x35S-Omega promoter for higher-level expression. The kanamycin- and hygromycin-resistant markers are independently available for each of the 43 types of vectors, thus an additional transformation of once-transformed plants can be carried out easily. Their small size and high-copy number in Escherichia coli make possible easier handling at plasmid preparation and sequencing. Improved pGWBs should be a powerful tool for transgenic research in plants.
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Cited by
Binder, A., Lambert, J., Morbitzer, R., Popp, C., Ott, T., Lahaye, T., & Parniske, M. (2014) · PLoS ONE · doi:10.1371/journal.pone.0088218 · Cited by 160
Characterization of MORE AXILLARY GROWTH Genes in Populus
Czarnecki, O., Yang, J., Wang, X., Wang, S., Muchero, W., Tuskan, G. A., & Chen, J.-G. (2014) · PLoS ONE · doi:10.1371/journal.pone.0102757 · Cited by 32
Attah, V., Milner, D. S., Fang, Y., Yan, X., Leonard, G., Heitman, J., Talbot, N. J., & Richards, T. A. (2024) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2218927121 · Cited by 11
Kanda, A., Otani, K., Ueda, T., Takahashi, T., & Motose, H. (2022) · doi:10.1101/2022.07.08.499237 · Cited by 6
Gaertner, N. F., Maio, F., Arroyo-Mateos, M., Luna, A. P., Sabarit, B., Kwaaitaal, M., Eltschkner, S., Prins, M., Bejarano, E. R., & van den Burg, H. A. (2025) · Journal of Virology · doi:10.1128/jvi.01286-25 · Cited by 1
Metadata
- Authors
- NAKAGAWA, T., SUZUKI, T., MURATA, S., NAKAMURA, S., HINO, T., MAEO, K., TABATA, R., KAWAI, T., TANAKA, K., NIWA, Y., WATANABE, Y., NAKAMURA, K., KIMURA, T., & ISHIGURO, S.
- Venue
- Bioscience, Biotechnology, and Biochemistry
- Publisher
- Oxford University Press (OUP)
- Published
- 2007-08-23
- Volume
- 71
- Issue
- 8
- Pages
- 2095-2100
- ISSN
- 0916-8451 (ISSN-L)
- 1347-6947
- OpenAlex
- W1980400357
- Cited by
- 957
- References
- 0
- FWCI
- 10.17
- Type
- journal article
- Language
- en
- Open access
- bronze
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