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Evidence that ultrafine titanium dioxide induces micronuclei and apoptosis in Syrian hamster embryo fibroblasts.
Open accessAbstract
Inhaled ultrafine titanium dioxide (UF-TiO2) particles cause pronounced pulmonary inflammation, in contrast to fine TiO2. Previous studies provide evidence for the production of reactive oxygen species by alveolar macrophages, after overloading with UF-TiO2 particles and cytotoxicity of UF-TiO2 in rat lung alveolar macrophages. UF-TiO2 also causes pulmonary fibrosis and lung tumors in rats. UF-TiO2 particles are photogenotoxic, but in general, information on the genotoxicity of UF-TiO2 is still limited. We studied the potential of UF-TiO2 (particle size less than or equal to 20 nm) and fine TiO2 (particle size > 200 nm) to induce chromosomal changes, which can be monitored by the formation of micronuclei (MN) in Syrian hamster embryo (SHE) cells. We also analyzed UF-TiO2-treated cells for apoptosis induction. The MN assay revealed a significant increase in MN induction (p less than or equal to 0.05) in SHE cells after treatment with UF-TiO2 (1.0 micro g/cm2) for 12 hr (mean, 24.5 MN/1,000 cells), 24 hr (mean, 31.13 MN/1,000 cells), 48 hr (mean, 30.8 MN/1,000 cells), 66 hr (mean, 31.2 MN/1,000 cells), and 72 hr (mean, 31.3 MN/1,000 cells). Bisbenzimide staining of the fixed cells revealed typical apoptotic structures (apoptotic bodies), and the apoptosis-specific "DNA ladder pattern" resulting from internucleosomal cleavage was identified by gel electrophoresis. Furthermore, transmission electron microscopy of the exposed cells revealed the typical chromatin compaction of apoptosis.
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Cited by
Gurr, J.-R., Wang, A. S. S., Chen, C.-H., & Jan, K.-Y. (2005) · Toxicology · doi:10.1016/j.tox.2005.05.007 · Cited by 1,022
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Reeves, J. F., Davies, S. J., Dodd, N. J. F., & Jha, A. N. (2008) · Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis · doi:10.1016/j.mrfmmm.2007.12.010 · Cited by 441
Hussain, S., Boland, S., Baeza-Squiban, A., Hamel, R., Thomassen, L. C. J., Martens, J. A., Billon-Galland, M. A., Fleury-Feith, J., Moisan, F., Pairon, J.-C., et al. (2009) · Toxicology · doi:10.1016/j.tox.2009.04.001 · Cited by 347
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Metadata
- Authors
- Rahman, Q., Lohani, M., Dopp, E., Pemsel, H., Jonas, L., Weiss, D. G., & Schiffmann, D.
- Venue
- Environmental Health Perspectives
- Publisher
- American Chemical Society (ACS)
- Published
- 2002-08
- Volume
- 110
- Issue
- 8
- Pages
- 797-800
- ISSN
- 0091-6765 (ISSN-L)
- 1552-9924
- OpenAlex
- W2071396517
- Cited by
- 382
- References
- 0
- FWCI
- 1.07
- Type
- journal article
- Language
- en
- Open access
- gold
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- Rahman, Q., Lohani, M., Dopp, E., Pemsel, H., Jonas, L., Weiss, D. G., & Schiffmann, D. (2002). Evidence that ultrafine titanium dioxide induces micronuclei and apoptosis in Syrian hamster embryo fibroblasts. Environmental Health Perspectives. https://doi.org/10.1289/ehp.02110797
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