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Storage Temperature Effects on Proteolysis in Alfalfa Silage
Abstract
ABSTRACT FIRST and third cutting alfalfa was ensiled in mini-silos (100 ml centrifuge tubes) at 40 or 55% dry matter and incubated at 15, 25 or 35 C for 40 days. In both trials and at both dry matter levels, the amount of proteolysis increased with storage temperature, averaging 10 percentage units as a fraction of total nitrogen between 15 and 35 C. Increasing storage temperature also elevated ammonia concentrations between 15 and 25 C but not between 25 and 35 C. The results of this study indicated that temperature affected the rate of loss of protease activity during fermentation, based on simulations using the silage model of Pitt et al. (1985) as modified by Muck (1987). The experiments suggest that management practices that control or reduce silage temperature may be important in maximizing the amount of nitrogen in alfalfa which remains as true protein through ensiling.
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“Proteolysis, the breakdown of plant proteins to free AA, peptides, and ammonia, is also affected by temperature, with rates doubling for every 10°C increase between 10 and 38°C (Muck and Dickerson, 1988).”
From Silage review: Factors affecting dry matter and quality losses in silages (2018) · cites this paper as Muck and Dickerson, 1988
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Metadata
- Authors
- Muck, R. E., & Dickerson, J. T.
- Venue
- Transactions of the ASAE
- Published
- 1988
- Volume
- 31
- Issue
- 4
- Pages
- 1005-1009
- ISSN
- 0001-2351 (ISSN-L)
- 2151-0059
- OpenAlex
- W1975613344
- Cited by
- 223
- References
- 0
- Citations by year
- 2026: 3 · 2025: 8 · 2024: 8 · 2023: 7 · 2022: 10 · 2021: 14 · 2020: 15 · 2019: 13 · 2018: 11 · 2017: 5 · 2016: 13 · 2015: 17 · 2014: 10 · 2013: 7 · 2012: 12
- FWCI
- 1.50
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Transactions of the ASAE (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Ruminant Nutrition and Digestive Physiology
- Physical Sciences › Engineering › Mechanical Engineering › Agricultural Engineering and Mechanization
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
- Keywords
- alfalfa silage, proteolysis, storage temperature, ammonia, proteolytic activity, silage fermentation, dry matter content, nitrogen retention, true protein
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