The DOI matches its Crossref record; 51 of the 52 references it cites were found in a registry or an index.
Loss of Osteocyte Integrity in Association with Microdamage and Bone Remodeling After Fatigue In Vivo
Abstract
As a result of fatigue, bone sustains microdamage, which is then repaired by bone-remodeling processes. How osteoclastic activity is targeted at the removal of microdamaged regions of bone matrix is unknown. In the current studies, we tested the hypothesis that changes in osteocyte integrity, through the initiation of regulated cell death (apoptosis), are associated with fatigue-related microdamage and bone resorption. Ulnae of adult rats were fatigue-loaded to produce a known degree of matrix damage. Osteocyte integrity was then assessed histomorphometrically from terminal deoxynucleotidyl transferase–mediated deoxyuridine triphosphate–nick end labeling (TUNEL)–stained sections to detect cells undergoing DNA fragmentation associated with apoptosis; toluidine blue–stained sections were used for secondary morphological confirmation. Ten days after loading, large numbers of TUNEL-positive osteocytes were found in bone surrounding microcracks and in bone surrounding intracortical resorption spaces (∼300% increases over controls, p < 0.005). TUNEL labeling in loaded ulnae at sites distant from microcracks or resorption foci did not differ from that in control bone. Osteocytes in toluidine blue–stained sections showed equivalent trends to TUNEL-stained sections, with significant increases in pyknotic nuclei and empty lacunae associated with microcracks and intracortical resorption spaces. TUNEL-positive osteocytes were observed around bone microdamage by 1 day after loading (p < 0.01 relative to baseline), and their number remained elevated throughout the entire experimental period. Increases in empty lacunae and decreases in normal osteocyte numbers were observed over time as well. These studies show that (1) osteocyte apoptosis is induced by bone fatigue, (2) this apoptosis is localized to regions of bone that contain microcracks, and (3) osteoclastic resorption after fatigue also coincides with regions of osteocyte apoptosis. The strong associations between microdamage, osteocyte apoptosis, and subsequent bone remodeling support the hypothesis that osteocyte apoptosis provides a key part of the activation or signaling mechanisms by which osteoclasts target bone for removal after fatigue-induced matrix injury.
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In the citing paper
“Osteocyte apoptosis has been shown to act as a chemotactic signal for local osteoclast recruitment [70, 150, 152, 190, 191].”
From Biology of Bone Tissue: Structure, Function, and Factors That Influence Bone Cells (2015) · cites this paper as [190]
In the citing paper
“In vivo anatomical studies have suggested that the presence of apoptotic osteocytes at microdamaged sites correlates with the recruitment of osteoclasts at microcracks.48”
From Bone remodeling: an operational process ensuring survival and bone mechanical competence (2022) · cites this paper as [48]
Citations
Cites
Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation.
Gavrieli, Y., Sherman, Y., & Ben-Sasson, S. A. (1992) · The Journal of cell biology · doi:10.1083/jcb.119.3.493 · Cited by 9,444
Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis
Nicholson, D. W., Ali, A., Thornberry, N. A., Vaillancourt, J. P., Ding, C. K., Gallant, M., Gareau, Y., Griffin, P. R., Labelle, M., Lazebnik, Y. A., et al. (1995) · Nature · doi:10.1038/376037a0 · Cited by 3,980
Fadok, V. A., Voelker, D. R., Campbell, P. A., Cohen, J., Bratton, D. L., & Henson, P. M. (1992) · The Journal of Immunology · doi:10.4049/jimmunol.148.7.2207 · Cited by 3,321
Savill, J. S., Wyllie, A. H., Henson, J. E., Walport, M. J., Henson, P. M., & Haslett, C. (1989) · Journal of Clinical Investigation · doi:10.1172/jci113970 · Cited by 1,536
Programmed Cell Death and the Control of Cell Survival: Lessons from the Nervous System
RAFF, M. C., Barres, B. A., Burne, J. F., Coles, H., Ishizaki, Y., & Jacobson, M. D. (1993) · Science · doi:10.1126/science.8235590 · Cited by 1,450
Suppression of ICE and Apoptosis in Mammary Epithelial Cells by Extracellular Matrix
Boudreau, N. J., Sympson, C. J., Werb, Z., & Bissell, M. J. (1995) · Science · doi:10.1126/science.7531366 · Cited by 1,174
Anchorage dependence, integrins, and apoptosis
Ruoslahti, E. I., & Reed, J. C. (1994) · Cell · doi:10.1016/0092-8674(94)90209-7 · Cited by 1,001
Sequential activation of ICE-like and CPP32-like proteases during Fas-mediated apoptosis
Enari, M., Talanian, R. V., Wrong, W. W., & Nagata, S. (1996) · Nature · doi:10.1038/380723a0 · Cited by 960
Vitronectin receptor-mediated phagocytosis of cells undergoing apoptosis
Savill, J., Dransfield, I., Hogg, N., & Haslett, C. (1990) · Nature · doi:10.1038/343170a0 · Cited by 846
Bone remodeling in response to in vivo fatigue microdamage
Burr, D. B., Martin, R. B., Schaffler, M. B., & Radin, E. L. (1985) · Journal of Biomechanics · doi:10.1016/0021-9290(85)90204-0 · Cited by 765
Self-citation
Research works printed but not linked: 3
- Qiu, 1997, Osteocyte apoptosis in reaction to matrix damage in compact bone Trans, Orthop Res Soc, 22, 89
- Noble, 1997, Bone loading changes the number and distribution of apoptotic osteocytes in cortical bone, J Bone Miner Res, 12, 36
- Noble, 1998, Matrix microdamage and osteocyte apoptosis: A mechanism for targeting bone resorption, Bone, 23, 1128
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Metadata
- Authors
- Verborgt, O., Gibson, G. J., & Schaffler, M. B.
- Affiliations
- University of Antwerp (BE)
- Henry Ford Health (US)
- Icahn School of Medicine at Mount Sinai (US)
- Corresponding author
- Schaffler, Mitchell B.
- Venue
- Journal of Bone and Mineral Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2000-01-01
- Volume
- 15
- Issue
- 1
- Pages
- 60-67
- ISSN
- 0884-0431 (ISSN-L)
- 1523-4681
- OpenAlex
- W2020238012
- Cited by
- 731
- References
- 52
- Citations by year
- 2026: 10 · 2025: 18 · 2024: 9 · 2023: 23 · 2022: 23 · 2021: 21 · 2020: 29 · 2019: 38 · 2018: 31 · 2017: 28 · 2016: 31 · 2015: 39 · 2014: 30 · 2013: 40 · 2012: 32
- FWCI
- 10.13
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
- Locations
- Journal of Bone and Mineral Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of Arthritis and Musculoskeletal and Skin Diseases (R01AR044712, AR41210, R01AR041210, AR 44712)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
- Health Sciences › Medicine › Orthopedics and Sports Medicine › Bone health and osteoporosis research
- Health Sciences › Medicine › Physiology (medical) › Spaceflight effects on biology
- Keywords
- osteocyte apoptosis, bone fatigue, microcracks, bone microdamage, bone remodeling, osteoclast resorption, ulna
- MeSH terms
- Animals, Female, Osteocytes, Stress, Physiological, Ulna, Bone Remodeling, Rats, Sprague-Dawley, In Situ Nick-End Labeling, Rats
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