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Two-photon fluorescence excitation cross sections of biomolecular probes from 690 to 960 nm
Abstract
We report on two-photon fluorescence excitation (TPE) action cross sections for five widely used molecular fluorophores. Measurements were performed by use of ultrashort (~100-fs) Ti:sapphire pulsed excitation over the range 690-960 nm. TPE spectra were obtained by comparison with a fluorescein calibration standard. Large cross sections were found for the cyanine reagent Cy 3 (~140 GM) and for Rhodamine 6G (~150 GM), both at 700 nm [1 GM = 10(-50) (cm(4) s)/photon]. Several fluorophores show interesting and desirable blue shifts with respect to twice the one-photon absorption wavelength. Fluorophore fluorescence intensities showed no significant departure (?4%) from quadratic illumination power dependence, indicating genuine two-photon processes. Implications of these measurements for two-photon laser-scanning microscopy are discussed.
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Cited by
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Two-photon excitation microscopy for the study of living cells and tissues
Benninger, R. K. P., & Piston, D. W. (2013) · Current Protocols in Cell Biology · doi:10.1002/0471143030.cb0411s59 · Cited by 311
Mongeon, R., Venkatachalam, V., & Yellen, G. (2016) · Antioxidants & Redox Signaling · doi:10.1089/ars.2015.6593 · Cited by 100
Khalin, I., Heimburger, D., Melnychuk, N., Collot, M., Groschup, B., Hellal, F., Reisch, A., Plesnila, N., & Klymchenko, A. S. (2020) · ACS Nano · doi:10.1021/acsnano.0c01505 · Cited by 82
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Metadata
- Authors
- Albota, M. A., Xu, C., & Webb, W. W.
- Venue
- Applied Optics
- Publisher
- Optica Publishing Group
- Published
- 1998-11-01
- Volume
- 37
- Issue
- 31
- Pages
- 7352
- ISSN
- 0003-6935 (ISSN-L)
- 1539-4522
- OpenAlex
- W2015886513
- Cited by
- 672
- References
- 0
- FWCI
- 6.15
- Type
- journal article
- Language
- en
- Open access
- closed
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- Albota, M. A., Xu, C., & Webb, W. W. (1998). Two-photon fluorescence excitation cross sections of biomolecular probes from 690 to 960 nm. Applied Optics. https://doi.org/10.1364/ao.37.007352
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