Imaging biological specimens with high-intensity soft x rays
Abstract
I compare alternative techniques for x-ray imaging of biological specimens on the basis of (1) transverse and longitudinal resolution, (2) depth of field, (3) choice of recording medium, and (4) recording efficiency. I show that, for all imaging techniques, the dosages suffered by specimens are so high that the living state cannot be preserved, nor can the structural integrity of the specimen be maintained in the usual sense. I demonstrate the remarkable fact that, at sufficiently high intensity, an image of diffraction-limited resolution can be captured before the specimen is obliterated.
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The 40 fs XFEL laser pulse enables 'diffraction before destruction' data collection, whereby the diffraction pattern is recorded before the sample is destroyed.
“Nonetheless, the 40 fs laser pulse enables the so-called “diffraction before destruction” data collection58,59, whereby the diffraction pattern is recorded before the sample is destroyed.”
From Architecture of the synaptotagmin–SNARE machinery for neuronal exocytosis (2015) · cites this paper as [59]
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Architecture of the synaptotagmin–SNARE machinery for neuronal exocytosis
Zhou, Q., Lai, Y., Bacaj, T., Zhao, M., Lyubimov, A. Y., Uervirojnangkoorn, M., Zeldin, O. B., Brewster, A. S., Sauter, N. K., Cohen, A. E., et al. (2015) · Nature · doi:10.1038/nature14975 · Cited by 348 · see citing works
Atomistic three-dimensional coherent x-ray imaging of nonbiological systems
Ho, P. J., Knight, C., Tegze, M., Faigel, G., Bostedt, C., & Young, L. (2016) · Physical Review A · doi:10.1103/physreva.94.063823 · Cited by 20 · see citing works
- Authors
- Solem, J. C.
- Affiliations
- Los Alamos National Laboratory (US)
- Corresponding author
- Solem, Johndale C.
- Venue
- Journal of the Optical Society of America B
- Publisher
- Optica Publishing Group
- Published
- 1986-11-01
- Volume
- 3
- Issue
- 11
- Pages
- 1551
- ISSN
- 0740-3224 (ISSN-L)
- 1520-8540
- OpenAlex
- W2129675664
- Cited by
- 92
- References
- 0
- Citations by year
- 2024: 1 · 2023: 1 · 2022: 1 · 2021: 1 · 2020: 5 · 2019: 21 · 2018: 2 · 2017: 5 · 2016: 9 · 2015: 6 · 2014: 1 · 2013: 3 · 2012: 3
- FWCI
- 2.73
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of the Optical Society of America B (publishedVersion)
- Topics
- Physical Sciences › Physics and Astronomy › Radiation › Advanced X-ray Imaging Techniques
- Physical Sciences › Physics and Astronomy › Atomic and Molecular Physics, and Optics › Digital Holography and Microscopy
- Physical Sciences › Engineering › Biomedical Engineering › Optical Coherence Tomography Applications
- Keywords
- soft X-ray imaging, biological specimen, diffraction-limited resolution, specimen damage, radiation dose, depth of field, recording media, recording efficiency, sample preservation
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