The DOI matches its Crossref record; 21 of the 21 references it cites were found in a registry or an index.
Two-photon excitation STED microscopy
Open accessAbstract
We report sub-diffraction resolution in two-photon excitation (TPE) fluorescence microscopy achieved by merging this technique with stimulated-emission depletion (STED). We demonstrate an easy-to-implement and promising laser combination based on a short-pulse laser source for two-photon excitation and a continuous-wave (CW) laser source for resolution enhancement. Images of fluorescent nanoparticles and the immunostained transcription regulator NF kappaB in mammalian cell nuclei exhibit resolutions of <50 nm and approximately 70 nm in the focal plane, respectively, corresponding to a 4-5.4-fold improvement over the diffraction barrier.
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“The STED principle can also be applied to two-photon microscopy (Moneron and Hell, 2009).”
From Barriers in the brain: resolving dendritic spine morphology and compartmentalization (2014) · cites this paper as Moneron and Hell, 2009
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Cites
Two-photon laser scanning fluorescence microscopy
Denk, W., Strickler, J. H., & Webb, W. W. (1990) · Science · doi:10.1126/science.2321027 · Cited by 9,488
Hell, S. W., & Wichmann, J. (1994) · Optics Letters · doi:10.1364/ol.19.000780 · Cited by 6,068
Self-citationDeep tissue two-photon microscopy
Helmchen, F., & Denk, W. T. (2005) · Nature Methods · doi:10.1038/nmeth818 · Cited by 4,288
STED microscopy reveals that synaptotagmin remains clustered after synaptic vesicle exocytosis
Willig, K. I., Rizzoli, S. O., Westphal, V., Jahn, R., & Hell, S. W. (2006) · Nature · doi:10.1038/nature04592 · Cited by 1,236
Self-citationPrinciples of two-photon excitation microscopy and its applications to neuroscience
Svoboda, K., & Yasuda, R. (2006) · Neuron · doi:10.1016/j.neuron.2006.05.019 · Cited by 1,027
Microscopy and its focal switch
Hell, S. W. (2008) · Nature Methods · doi:10.1038/nmeth.1291 · Cited by 999
Self-citationSubdiffraction resolution in far-field fluorescence microscopy
Klar, T. A., & Hell, S. W. (1999) · Optics Letters · doi:10.1364/ol.24.000954 · Cited by 866
Self-citationSTED microscopy reveals crystal colour centres with nanometric resolution
Rittweger, E., Han, K. Y., Irvine, S. E., Eggeling, C., & Hell, S. W. (2009) · Nature Photonics · doi:10.1038/nphoton.2009.2 · Cited by 826
Self-citationVideo-Rate Far-Field Optical Nanoscopy Dissects Synaptic Vesicle Movement
Westphal, V., Rizzoli, S. O., Lauterbach, M. A., Kamin, D., Jahn, R., & Hell, S. W. (2008) · Science · doi:10.1126/science.1154228 · Cited by 799
Self-citationMacromolecular-scale resolution in biological fluorescence microscopy
Donnert, G., Keller‐Findeisen, J., Medda, R., Andrei, M. A., Rizzoli, S. O., Lührmann, R., Jahn, R., Eggeling, C., & Hell, S. W. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0604965103 · Cited by 528
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Breaking the Diffraction Barrier: Super-Resolution Imaging of Cells
Huang, B., Babcock, H. P., & Zhuang, X. (2010) · Cell · doi:10.1016/j.cell.2010.12.002 · Cited by 1,174
Two-photon absorption properties of fluorescent proteins
Drobizhev, M. A., Makarov, N. S., Tillo, S., Hughes, T. E., & Rebane, A. K. (2011) · Nature Methods · doi:10.1038/nmeth.1596 · Cited by 677
Advanced Fluorescence Microscopy Techniques—FRAP, FLIP, FLAP, FRET and FLIM
Ishikawa-Ankerhold, H. C., Ankerhold, R., & Drummen, G. P. C. (2012) · Molecules · doi:10.3390/molecules17044047 · Cited by 562
Sharper low-power STED nanoscopy by time gating
Vicidomini, G., Moneron, G., Han, K. Y., Westphal, V., Ta, H., Reuss, M., Engelhardt, J., Eggeling, C., & Hell, S. W. (2011) · Nature Methods · doi:10.1038/nmeth.1624 · Cited by 423
Self-citationStimulated Emission Depletion Microscopy
Blom, H., & Widengren, J. (2017) · Chemical Reviews · doi:10.1021/acs.chemrev.6b00653 · Cited by 358
Metadata
- Authors
- Moneron, G., & Hell, S. W.
- ORCID
- Hell, Stefan W.
- Venue
- Optics Express
- Publisher
- Optica Publishing Group
- Published
- 2009-08-03
- Volume
- 17
- Issue
- 17
- Pages
- 14567
- ISSN
- 1094-4087 (ISSN-L)
- OpenAlex
- W2152298085
- Cited by
- 238
- References
- 21
- Citations by year
- 2026: 7 · 2025: 4 · 2024: 6 · 2023: 10 · 2022: 11 · 2021: 11 · 2020: 12 · 2019: 15 · 2018: 19 · 2017: 18 · 2016: 15 · 2015: 18 · 2014: 25 · 2013: 18 · 2012: 18
- FWCI
- 9.49
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Optics Express (publishedVersion)
- PubMed (publishedVersion)
- Max Planck Institute for Plasma Physics (submittedVersion)
- GRO (submittedVersion)
- MPG.PuRe (Max Planck Society) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
- Physical Sciences › Physics and Astronomy › Atomic and Molecular Physics, and Optics › Laser-Matter Interactions and Applications
- Physical Sciences › Materials Science › Electronic, Optical and Magnetic Materials › Nonlinear Optical Materials Studies
- Keywords
- two-photon excitation, STED microscopy, stimulated emission depletion, sub-diffraction resolution, super-resolution fluorescence microscopy, short-pulse lasers, continuous-wave laser, fluorescent nanoparticles, NF-κB, immunostaining
- MeSH terms
- Animals, Cell Nucleus, Fluorescent Dyes, Humans, Lasers, Microscopy, Electron, Scanning, Microscopy, Fluorescence, Models, Statistical, NF-kappa B, Photons, Microscopy, Confocal, Imaging, Three-Dimensional, Nanotechnology, Nanoparticles
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