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Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength axial accuracy

Open access
Marquet, Pierre, Rappaz, Benjamin, Magistretti, Pierre J., Cuche, Etienne, Emery, Yves, Colomb, Tristan, Depeursinge, Christian·2005·Optics Letters

Abstract

We have developed a digital holographic microscope (DHM), in a transmission mode, especially dedicated to the quantitative visualization of phase objects such as living cells. The method is based on an original numerical algorithm presented in detail elsewhere [Cuche et al., Appl. Opt. 38, 6994 (1999)]. DHM images of living cells in culture are shown for what is to our knowledge the first time. They represent the distribution of the optical path length over the cell, which has been measured with subwavelength accuracy. These DHM images are compared with those obtained by use of the widely used phase contrast and Nomarski differential interference contrast techniques.

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Metadata

Authors
Marquet, P., Rappaz, B., Magistretti, P. J., Cuche, E., Emery, Y., Colomb, T., & Depeursinge, C.
Venue
Optics Letters
Published
2005-03-01
Volume
30
Issue
5
Pages
468
ISSN
  • 0146-9592 (ISSN-L)
  • 1539-4794
OpenAlex
W2015526235
Cited by
1,254
References
7
FWCI
24.80
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength axial accuracy. https://app.cochat.ai/papers/10.1364/ol.30.000468
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Marquet, P., Rappaz, B., Magistretti, P. J., Cuche, E., Emery, Y., Colomb, T., & Depeursinge, C. (2005). Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength axial accuracy. Optics Letters. https://doi.org/10.1364/ol.30.000468

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