CoChat
Sign Up
Cited by 2,094

Optimized survival of hippocampal neurons in B27‐supplemented neurobasal™, a new serum‐free medium combination

Brewer, G. J., Torricelli, J. R., Evege, E. K., Price, P. J.·1993·Journal of Neuroscience Research
DOI 10.1002/jnr.490350513
Open this paper in CoChat — free account

Abstract

We have systematically optimized the concentrations of 20 components of a previously published serumfree medium (Brewer and Cotman, Brain Res 494: 65‐74, 1989) for survival of rat embryonic hippocampal neurons after 4 days in culture. This serum‐freemedium Supplement, B27, produced neuron survival above 60% independent of plating density above 160 plated cells/mm 2 For isolated cells (<100 cells/mm 2 ), survival at 4 days was still above 45%, but could be rescued to the 60% level at 40 cells/mm 2 by simply applying a coverslip on top of the cells. This suggests a need for additional trophic factors. High survival was achieved with osmolarity lower than found in Dulbecco's Modified Eagle's Medium (DMEM), and by reducing cysteine and glutamine concentrations and by the elimination of toxic ferrnous sulphate found in DME/F12. Neurobasal is a new medium that incorporates these modifications to DMEM. In B27/ Neurobasal, glial growth is reduced to less than 0.5% of the nearly pure neuronal population, as judged by immunocytochemistry for glial fibrillary acidic protein and neuron‐specific enolase. Excellent long‐term viability is achieved after 4 weeks in culture with greater than 90%viability for cells plated at 640/mm 2 and greater than 50 viability for cells plated at 160/mm 2 Since the medium also supports the growth of neurons from embryonic rat striatum, substantia nigra, septum, and cortex, and neonatal dentate gyrus and cerebellum (Brewer, in preparation), support for other neuron types is likely. B27/Neurobasal should be useful for in vitro studies of neuronal toxicology, pharmacology, electrophysiology, gene expression, development, and effects of growth factors and hormones. © 1993 Wiley‐Liss, Inc.

Integrity signals

Registry

Crossref · 10.1002/jnr.490350513

Cites

1 of 1 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Brewer, G. J., Torricelli, J. R., Evege, E. K., & Price, P. J.
Venue
Journal of Neuroscience Research
Publisher
Wiley
Published
1993-08
Volume
35
Issue
5
Pages
567-576
ISSN
  • 0360-4012
  • 1097-4547
DOI
10.1002/jnr.490350513
Cited by
2,094
References
1
Language
en
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor, 4089 d)

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review